scholarly journals Comparison of the Quantiplex Version 3.0 Assay and a Sensitized Amplicor Monitor Assay for Measurement of Human Immunodeficiency Virus Type 1 RNA Levels in Plasma Samples

1999 ◽  
Vol 37 (11) ◽  
pp. 3612-3614 ◽  
Author(s):  
Helene C. Highbarger ◽  
W. Gregory Alvord ◽  
Min Kang Jiang ◽  
Akram S. Shah ◽  
Julia A. Metcalf ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Rt Pcr ◽  
Hiv Rna ◽  
Immunodeficiency Virus ◽  
Highly Correlated ◽  
The Relationship ◽  
Hiv 1

This study evaluated correlation and agreement between version 3 of the Quantiplex human immunodeficiency virus type 1 (HIV-1) RNA assay (v3 branched DNA [bDNA]) and a sensitized Amplicor HIV-1 Monitor assay (reverse transcription [RT]-PCR) for the measurement of HIV RNA. Three hundred eighteen samples from 59 randomly selected, HIV-1-seropositive persons on various drug protocols from the National Institute of Allergy and Infectious Diseases HIV outpatient clinic were studied. The results indicate that v3 bDNA and RT-PCR are highly correlated (r = 0.98) and are in good agreement (mean difference in log10 copies/ml ± 2 standard deviations = 0.072 ± 0.371). The relationship between values obtained by both assays is given by the following equation: log10v3 bDNA = −0.0915 + 1.0052 · log10RT-PCR. This represents a 1.026-fold difference between log10RT-PCR values and log10v3 bDNA values.

scholarly journals Earlier Detection of Human Immunodeficiency Virus Type 1 p24 Antigen and Immunoglobulin G and M Antibodies to p17 Antigen in Seroconversion Serum Panels by Immune Complex Transfer Enzyme Immunoassays

2000 ◽  
Vol 7 (6) ◽  
pp. 872-881 ◽  
Author(s):  
Seiichi Hashida ◽  
Setsuko Ishikawa ◽  
Kazuya Hashinaka ◽  
Ichiro Nishikata ◽  
Shinichi Oka ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Immune Complex ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Immunoglobulin G ◽  
Rt Pcr ◽  
Immunodeficiency Virus ◽  
P24 Antigen ◽  
Hiv 1

ABSTRACT For earlier diagnosis of human immunodeficiency virus type 1 (HIV-1) infection, the sensitivities of immune complex transfer enzyme immunoassays for HIV-1 p24 antigen and antibody immunoglobulin G (IgG) to HIV-1 p17 antigen were improved approximately 25- and 90-fold, respectively, over those of the previous immunoassays by performing solid-phase immunoreactions with shaking and increasing the serum sample volumes, and immune complex transfer enzyme immunoassay of antibody IgM to p17 antigen was also performed in the same way as the improved immunoassay of antibody IgG to p17 antigen. By the improved immunoassays, p24 antigen and antibody IgG to p17 antigen were detected earlier in 32 and 53%, respectively, of the HIV-1 seroconversion serum panels tested than before the improvements, and p24 antigen was detected as early as or earlier than HIV-1 RNA by reverse transcriptase-PCR (RT-PCR) in all of the panels tested. In 4 panels out of 19 tested, antibody IgG to p17 antigen or both antibodies IgG and IgM to p17 antigen were detected earlier than p24 antigen and RNA, although the antibody levels declined slightly before their steep increases usually observed after p24 antigen and RNA. Thus, the window period in diagnosis of HIV-1 infection can be shortened by detection of p24 antigen with the improved immunoassay as much as by detection of RNA with RT-PCR and, in some cases, more by detection of antibodies IgG and IgM to p17 antigen with the improved immunoassays than by detections of p24 antigen with the improved immunoassay and RNA with RT-PCR.

scholarly journals Quantitative and Cost Comparison of Ultrasensitive Human Immunodeficiency Virus Type 1 RNA Viral Load Assays: Bayer bDNA Quantiplex Versions 3.0 and 2.0 and Roche PCR Amplicor Monitor Version 1.5

2000 ◽  
Vol 38 (3) ◽  
pp. 1113-1120 ◽  
Author(s):  
Tarek Elbeik ◽  
Edwin Charlebois ◽  
Patricia Nassos ◽  
James Kahn ◽  
Frederick M. Hecht ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Viral Load ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Cost Comparison ◽  
Highly Active ◽  
Immunodeficiency Virus ◽  
Highly Correlated ◽  
Hiv 1

Quantification of human immunodeficiency virus type 1 (HIV-1) RNA as a measure of viral load has greatly improved the monitoring of therapies for infected individuals. With the significant reductions in viral load now observed in individuals treated with highly active anti-retroviral therapy (HAART), viral load assays have been adapted to achieve greater sensitivity. Two commercially available ultrasensitive assays, the Bayer Quantiplex HIV-1 bDNA version 3.0 (bDNA 3.0) assay and the Roche Amplicor HIV-1 Monitor Ultrasensitive version 1.5 (Amplicor 1.5) assay, are now being used to monitor HIV-1-infected individuals. Both of these ultrasensitive assays have a reported lower limit of 50 HIV-1 RNA copies/ml and were developed from corresponding older generation assays with lower limits of 400 to 500 copies/ml. However, the comparability of viral load data generated by these ultrasensitive assays and the relative costs of labor, disposables, and biohazardous wastes were not determined in most cases. In this study, we used matched clinical plasma samples to compare the quantification of the newer bDNA 3.0 assay with that of the older bDNA 2.0 assay and to compare the quantification and costs of the bDNA 3.0 assay and the Amplicor 1.5 assay. We found that quantification by the bDNA 3.0 assay was approximately twofold higher than that by the bDNA 2.0 assay and was highly correlated to that by the Amplicor 1.5 assay. Moreover, cost analysis based on labor, disposables, and biohazardous wastes showed significant savings with the bDNA 3.0 assay as compared to the costs of the Amplicor 1.5 assay.

scholarly journals Efficient Isolation of Human Immunodeficiency Virus Type 1 RNA from Cervical Swabs

1998 ◽  
Vol 36 (8) ◽  
pp. 2349-2352 ◽  
Author(s):  
Adeline M. Hajjar ◽  
Paul F. Lewis ◽  
Yohannes Endeshaw ◽  
Jackoniah Ndinya-Achola ◽  
Joan K. Kreiss ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Functional Studies ◽  
Hiv Rna ◽  
Immunodeficiency Virus ◽  
Quantitative Analyses ◽  
Mucosal Secretions ◽  
Hiv 1

An efficient method for the isolation of human immunodeficiency virus type 1 (HIV-1) nucleic acids from dry cervical swabs was developed. HIV-1 gag and env were detected in 96% (25 of 26) and 81% (21 of 26), respectively, of the samples tested by PCR from HIV-1-seropositive women in a Kenyan cohort study. Eighty-eight percent of the swabs (22 of 25) were positive forgag RNA, and 85% (17 of 20) were positive forenv RNA. Fewer than 1,000 copies of HIV-1 gagRNA were detected in four swabs in which a competitive quantitative PCR assay was used. The method described here may be useful for both qualitative and quantitative analyses of HIV RNA in mucosal secretions as well as amplification and cloning of full-length viral genes for functional studies.

Spread of distinct human immunodeficiency virus type 1 AG recombinant lineages in Africa

Microbiology ◽  
2000 ◽  
Vol 81 (2) ◽  
pp. 515-523 ◽  
Author(s):  
Marion Cornelissen ◽  
Remco van den Burg ◽  
Fokla Zorgdrager ◽  
Jaap Goudsmit
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Rt Pcr ◽  
Serum Samples ◽  
African Countries ◽  
Immunodeficiency Virus ◽  
Subtype A ◽  
Hiv 1

To identify new subtype G human immunodeficiency virus type 1 (HIV-1) strains and AG recombinant forms, we collected 28 serum samples from immigrants to the Netherlands from 12 countries throughout Africa. Based on the gag sequences 22 isolates were identified as subtype A or G. Phylogenetic analysis of discontinuous regions of the gag (726 nt), pol (1176 nt) and env (276 nt) genes revealed 13 AG recombinants with the mosaic structure A gag /G pol /A env , three with A gag /G pol /G env and one other with A gag /G pol /G env , in addition to ‘pure’ subtypes A gag /A pol /A env (n=1) and G gag /G pol /G env (n=4). To analyse the crossover points in more detail, a new RT–PCR was developed resulting in a large contiguous sequence of 2600 nt from the gag region to half the pol region. All the 13 A gag /G pol /A env recombinants appeared to belong to the circulating recombinant form (CRF) AG (IbNG). The three A gag /G pol /G env recombinants differed from the CRF AG (IbNG) subtype, suggesting the identification of a new CRF subtype. The recovery of AG recombinants from African countries a thousand miles apart indicates the active spread of new recombinants.

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