scholarly journals Quality Control Limits for Broth Microdilution Susceptibility Tests of Ten Antifungal Agents

2000 ◽  
Vol 38 (9) ◽  
pp. 3457-3459 ◽  
Author(s):  
A. L. Barry ◽  
M. A. Pfaller ◽  
S. D. Brown ◽  
A. Espinel-Ingroff ◽  
M. A. Ghannoum ◽  
...  
Keyword(s):  
Quality Control ◽  
Antifungal Agents ◽  
Candida Parapsilosis ◽  
Clinical Laboratory ◽  
Collaborative Study ◽  
Candida Krusei ◽  
National Committee ◽  
Broth Microdilution ◽  
Susceptibility Tests ◽  
Control Limits

Broth microdilution susceptibility tests of Candidaspecies have now been standardized by the National Committee for Clinical Laboratory Standards (NCCLS). An eight-laboratory collaborative study was carried out in order to document reproducibility of tests of Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258 by the NCCLS method. Replicate broth microdilution tests were used to define control limits for 24- and 48-h MICs of amphotericin B, flucytosine, fluconazole, voriconazole, ketoconazole, itraconazole, caspofungin (MK 0991), ravuconazole (BMS 207147), posaconazole (SCH 56592), and LY 303366.

scholarly journals Quality Control Guidelines for Disk Diffusion and Broth Microdilution Antimicrobial Susceptibility Tests with Seven Drugs for Veterinary Applications

2000 ◽  
Vol 38 (1) ◽  
pp. 453-455
Author(s):  
Brant A. Odland ◽  
Meredith E. Erwin ◽  
Ronald N. Jones
Keyword(s):  
Quality Control ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Clinical Laboratory ◽  
Disk Diffusion ◽  
National Committee ◽  
Broth Microdilution ◽  
E Coli ◽  
Antimicrobial Susceptibility Tests ◽  
Susceptibility Tests

ABSTRACT This multicenter study proposes antimicrobial susceptibility (MIC and disk diffusion methods) quality control (QC) parameters for seven compounds utilized in veterinary health. Alexomycin, apramycin, tiamulin, tilmicosin, and tylosin were tested by broth microdilution against various National Committee for Clinical Laboratory Standards (NCCLS)-recommended QC organisms ( Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Streptococcus pneumoniae ATCC 49619, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853). In addition, disk diffusion zone diameter QC limits were determined for apramycin, enrofloxacin, and premafloxacin by using E. coli ATCC 25922, P. aeruginosa ATCC 27853, and S. aureus ATCC 25923. The results from five or six participating laboratories produced ≥99.0% of MICs and ≥95.0% of the zone diameters within suggested guidelines. The NCCLS Subcommittee for Veterinary Antimicrobial Susceptibility Testing has recently approved these ranges for publication in the next M31 document.

scholarly journals Quality Control Limits for Dilution and Disk Diffusion Susceptibility Tests of Trovafloxacin against Eight Quality Control Strains

1998 ◽  
Vol 36 (2) ◽  
pp. 585-586
Author(s):  
Peter C. Fuchs ◽  
Arthur L. Barry ◽  
Steven D. Brown
Keyword(s):  
Quality Control ◽  
Disk Diffusion ◽  
Collaborative Effort ◽  
Broth Microdilution ◽  
Agar Dilution ◽  
Susceptibility Tests ◽  
And Control ◽  
Control Limits

A 10-laboratory collaborative effort was designed to generate data to propose quality control limits for susceptibility tests of trovafloxacin. Broth microdilution, agar dilution, and disk diffusion tests were evaluated with eight different control strains. All tests were reproducible, and control limits are proposed.

Quality control limits for disk diffusion and broth microdilution susceptibility tests with Haemophilus test medium

1991 ◽  
Vol 14 (6) ◽  
pp. 485-493 ◽  
Author(s):  
Gary V. Doern ◽  
E.Hugh Gerlach ◽  
James H. Jorgensen ◽  
Patrick R. Murray ◽  
Clyde Thornsberry ◽  
...  
Keyword(s):  
Quality Control ◽  
Disk Diffusion ◽  
Broth Microdilution ◽  
Test Medium ◽  
Susceptibility Tests ◽  
Control Limits

scholarly journals In Vitro Susceptibility Tests for Cationic Peptides: Comparison of Broth Microdilution Methods for Bacteria That Grow Aerobically

2000 ◽  
Vol 44 (6) ◽  
pp. 1694-1696 ◽  
Author(s):  
A. Giacometti ◽  
O. Cirioni ◽  
F. Barchiesi ◽  
M. S. Del Prete ◽  
M. Fortuna ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Aerobic Bacteria ◽  
National Committee ◽  
Cationic Peptides ◽  
Broth Microdilution ◽  
In Vitro Susceptibility ◽  
Cecropin P1 ◽  
Magainin Ii ◽  
Susceptibility Tests

ABSTRACT The in vitro susceptibilities of 90 clinical isolates of gram-positive and gram-negative aerobic bacteria to six cationic peptides, buforin II, cecropin P1, indolicidin, magainin II, nisin, and ranalexin, were evaluated by two broth microdilution methods. The first method was performed according to the procedures outlined by the National Committee for Clinical Laboratory Standards for bacteria that grow aerobically, while the second was performed according to the procedures recently proposed by the R. E. W. Hancock laboratory for testing antimicrobial peptides. Overall, the first method produced MICs two- and fourfold higher than the second method.

In Vitro Activities of Voriconazole (UK-109,496) and Four Other Antifungal Agents against 394 Clinical Isolates ofCandida spp.

1998 ◽  
Vol 42 (1) ◽  
pp. 161-163 ◽  
Author(s):  
F. Marco ◽  
M. A. Pfaller ◽  
S. Messer ◽  
R. N. Jones
Keyword(s):  
Amphotericin B ◽  
Antifungal Agents ◽  
Clinical Laboratory ◽  
Candida Krusei ◽  
Antifungal Drugs ◽  
National Committee ◽  
In Vitro Activity ◽  
Medical Centers

ABSTRACT Voriconazole (formerly UK-109,496) is a new monotriazole antifungal agent which has potent activity against Candida,Cryptococcus, and Aspergillus species. We investigated the in vitro activity of voriconazole compared to those of fluconazole, itraconazole, amphotericin B, and flucytosine (5FC) against 394 bloodstream isolates of Candida (five species) obtained from more than 30 different medical centers. MICs of all antifungal drugs were determined by the method recommended by the National Committee for Clinical Laboratory Standards using RPMI 1640 test medium. Overall, voriconazole was quite active against all the yeast isolates (MIC at which 90% of the isolates are inhibited [MIC90], ≤0.5 μg/ml). Candida albicans was the most susceptible species (MIC90, 0.06 μg/ml) andCandida glabrata and Candida krusei were the least (MIC90, 1 μg/ml). Voriconazole was more active than amphotericin B and 5FC against all species except C. glabrata and was also more active than itraconazole and fluconazole. For isolates of Candida spp. with decreased susceptibility to fluconazole and itraconazole MICs of voriconazole were also higher. Based on these results, voriconazole has promising antifungal activity and further in vitro and in vivo investigations are warranted.

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