Genetic Diversity of Norovirus and Sapovirus in Hospitalized Infants with Sporadic Cases of Acute Gastroenteritis in Chiang Mai, Thailand

SUMMARYSapoviruses (SaVs) are responsible for sporadic cases and outbreaks of acute gastroenteritis. Despite this, few studies in India have focused on the epidemiological investigation of SaV in cases of acute gastroenteritis. The aim of this study was to understand the molecular epidemiology, genetic diversity and clinical impact of SaV in diarrhoeic children from Pune, Western India. Between 2007 and 2011, a total of 985 faecal samples from diarrhoeic cases and non-diarrhoeic controls were collected and examined for the presence of SaV by nested RT–PCR. SaV was detected in 2·7% (21/778) of the cases and 1·9% (4/207) of the controls. We observed that the majority of SaV mono-infections caused severe gastroenteritis (67%) with clinical manifestations of diarrhoea (100%), vomiting (73%) and dehydration (80%). All known human SaV genogroups were detected in the study. At least eight genotypes were identified from cases and controls. Genogroups GIV and GV, along with genotypes GI.5, GII.4 and GII.6, were discovered for the first time in India. Two GII.4 study strains were found to be 98·5–99% identical, having a novel intra-genogroup recombinant (GII.1/GII.4) recently reported from the Philippines, suggesting probable evidence of recombination. The circulation pattern of SaV genotypes varied during the study period, with GII.1 being predominant in 2007 and 2009, GIV.1 in 2008, and GV.1 in 2011.

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Genetic diversity of sapovirus in non-hospitalized adults with sporadic cases of acute gastroenteritis in Shanghai, China

Journal of Clinical Virology ◽

10.1016/j.jcv.2014.01.007 ◽

2014 ◽

Vol 59 (4) ◽

pp. 250-254 ◽

Cited By ~ 16

Author(s):

Gang Wang ◽

Zhen Shen ◽

Fangxing Qian ◽

Yang Li ◽

Zhenghong Yuan ◽

...

Keyword(s):

Genetic Diversity ◽

Acute Gastroenteritis ◽

Sporadic Cases

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Molecular Epidemiology of Oyster-Related Human Noroviruses and Their Global Genetic Diversity and Temporal-Geographical Distribution from 1983 to 2014

Applied and Environmental Microbiology ◽

10.1128/aem.01729-15 ◽

2015 ◽

Vol 81 (21) ◽

pp. 7615-7624 ◽

Cited By ~ 32

Author(s):

Yongxin Yu ◽

Hui Cai ◽

Linghao Hu ◽

Rongwei Lei ◽

Yingjie Pan ◽

...

Keyword(s):

Genetic Diversity ◽

Quality Control ◽

Molecular Epidemiology ◽

Geographical Distribution ◽

Acute Gastroenteritis ◽

Phylogenetic Analyses ◽

Disease Outbreaks ◽

Temporal Distribution ◽

Global Scale ◽

Sporadic Cases

ABSTRACTNoroviruses (NoVs) are a leading cause of epidemic and sporadic cases of acute gastroenteritis worldwide. Oysters are well recognized as the main vectors of environmentally transmitted NoVs, and disease outbreaks linked to oyster consumption have been commonly observed. Here, to quantify the genetic diversity, temporal distribution, and circulation of oyster-related NoVs on a global scale, 1,077 oyster-related NoV sequences deposited from 1983 to 2014 were downloaded from both NCBI GenBank and the NoroNet outbreak database and were then screened for quality control. A total of 665 sequences with reliable information were obtained and were subsequently subjected to genotyping and phylogenetic analyses. The results indicated that the majority of oyster-related NoV sequences were obtained from coastal countries and regions and that the numbers of sequences in these regions were unevenly distributed. Moreover, >80% of human NoV genotypes were detected in oyster samples or oyster-related outbreaks. A higher proportion of genogroup I (GI) (34%) was observed for oyster-related sequences than for non-oyster-related outbreaks, where GII strains dominated with an overwhelming majority of >90%, indicating that the prevalences of GI and GII are different in humans and oysters. In addition, a related convergence of the circulation trend was found between oyster-related NoV sequences and human pandemic outbreaks. This suggests that oysters not only act as a vector of NoV through environmental transmission but also serve as an important reservoir of human NoVs. These results highlight the importance of oysters in the persistence and transmission of human NoVs in the environment and have important implications for the surveillance of human NoVs in oyster samples.

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Genetic diversity of noroviruses and sapoviruses in children hospitalized with acute gastroenteritis in Chiang Mai, Thailand

Journal of Medical Virology ◽

10.1002/jmv.21004 ◽

2007 ◽

Vol 79 (12) ◽

pp. 1921-1926 ◽

Cited By ~ 39

Author(s):

Pattara Khamrin ◽

Niwat Maneekarn ◽

Supatra Peerakome ◽

Supin Tonusin ◽

Rungnapa Malasao ◽

...

Keyword(s):

Genetic Diversity ◽

Acute Gastroenteritis ◽

Chiang Mai

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Molecular epidemiology and genetic diversity of norovirus infection in children with acute gastroenteritis in Bangladesh, 2014‐2019

Journal of Medical Virology ◽

10.1002/jmv.26772 ◽

2021 ◽

Author(s):

Shuvra Kanti Dey ◽

Nadim Sharif ◽

Baki Billah ◽

Tanjir Tarek Ibn Siddique ◽

Tarequl Islam ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Epidemiology ◽

Acute Gastroenteritis

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Human bocavirus genotypes 1, 2, and 3 circulating in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand, 2012–2018

Journal of Infection and Public Health ◽

10.1016/j.jiph.2020.12.007 ◽

2021 ◽

Vol 14 (2) ◽

pp. 179-186

Author(s):

Nattika Nantachit ◽

Pakawat Kochjan ◽

Pattara Khamrin ◽

Kattareeya Kumthip ◽

Niwat Maneekarn

Keyword(s):

Acute Gastroenteritis ◽

Pediatric Patients ◽

Human Bocavirus ◽

Chiang Mai

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Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019–2020)

Pathogens ◽

10.3390/pathogens10060674 ◽

2021 ◽

Vol 10 (6) ◽

pp. 674

Author(s):

Anton Yuzhakov ◽

Ksenia Yuzhakova ◽

Nadezhda Kulikova ◽

Lidia Kisteneva ◽

Stanislav Cherepushkin ◽

...

Keyword(s):

Infectious Disease ◽

Genetic Diversity ◽

Acute Gastroenteritis ◽

Rt Pcr ◽

Clinical Hospital ◽

Group A Rotavirus ◽

Group A ◽

First Time ◽

Common Combination ◽

Children Under 5

Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.

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