Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019–2020)

Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.

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Genetic diversity of group A rotavirus in acute gastroenteritis outpatients in Shanghai from 2017 to 2018

BMC Infectious Diseases ◽

10.1186/s12879-020-05279-x ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Xiaozhou Kuang ◽

Xiaohuan Gong ◽

Xi Zhang ◽

Hao Pan ◽

Zheng Teng

Keyword(s):

Genetic Diversity ◽

Acute Gastroenteritis ◽

Group A Rotavirus ◽

Group A

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The Prevalence and Genotypic Distribution of Group A Rotavirus Detected from Patients with Acute Gastroenteritis Patients in Incheon

Journal of Bacteriology and Virology ◽

10.4167/jbv.2007.37.1.39 ◽

2007 ◽

Vol 37 (1) ◽

pp. 39 ◽

Cited By ~ 7

Author(s):

Jea-Mann Lee ◽

Hye-young Kim ◽

Mi-yeon Lee ◽

Kang-bum Lee ◽

Doo-Sung Cheon ◽

...

Keyword(s):

Acute Gastroenteritis ◽

Group A Rotavirus ◽

Genotypic Distribution ◽

Group A

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FIRST MOLECULAR DETECTION AND VP7 (G) GENOTYPING OF GROUP A ROTAVIRUS BY SEMI-NESTED RT-PCR FROM SEWAGE IN NIGERIA

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s1678-9946201658074 ◽

2016 ◽

Vol 58 (0) ◽

Cited By ~ 4

Author(s):

Babatunde Olanrewaju MOTAYO ◽

Adekunle Johnson ADENIJI ◽

Adedayo Omotayo FANEYE

Keyword(s):

Molecular Detection ◽

Rt Pcr ◽

Group A Rotavirus ◽

Group A

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Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus

Journal of Virological Methods ◽

10.1016/j.jviromet.2010.08.008 ◽

2010 ◽

Vol 169 (2) ◽

pp. 375-379 ◽

Cited By ~ 13

Author(s):

Karen Miyuki Asano ◽

Sibele Pinheiro de Souza ◽

Iracema Nunes de Barros ◽

Giselle Razera Ayres ◽

Sheila Oliveira Souza Silva ◽

...

Keyword(s):

Internal Control ◽

Simultaneous Detection ◽

Rt Pcr ◽

Bovine Coronavirus ◽

Group A Rotavirus ◽

Group A ◽

Exogenous Internal Control

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Genetic characterization of group A rotavirus strains circulating among children with acute gastroenteritis in Japan in 2004–2005

Infection Genetics and Evolution ◽

10.1016/j.meegid.2006.10.002 ◽

2007 ◽

Vol 7 (2) ◽

pp. 247-253 ◽

Cited By ~ 12

Author(s):

Tung Gia Phan ◽

Pattara Khamrin ◽

Trinh Duy Quang ◽

Shuvra Kanti Dey ◽

Fumihiro Yagyu ◽

...

Keyword(s):

Acute Gastroenteritis ◽

Genetic Characterization ◽

Group A Rotavirus ◽

Group A

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Epidemiology and genetic diversity of human parechoviruses circulating among children hospitalised with acute gastroenteritis in Pune, Western India: a 5-years study

Epidemiology and Infection ◽

10.1017/s095026881700262x ◽

2017 ◽

Vol 146 (1) ◽

pp. 11-18 ◽

Cited By ~ 12

Author(s):

P. R. PATIL ◽

N. N. GANORKAR ◽

V. GOPALKRISHNA

Keyword(s):

Genetic Diversity ◽

Acute Gastroenteritis ◽

Clinical Manifestations ◽

Western India ◽

Vp1 Gene ◽

Rt Pcr ◽

Chain Reaction ◽

Predominant Genotype ◽

Stool Specimens ◽

Polymerase Chain

SUMMARYHuman parechoviruses (HPeVs) are known to cause various clinical manifestations including acute gastroenteritis. Although HPeV infections and their genotypes have been detected in human patients worldwide, no such reports are available from India to ascertain the association of HPeVs in acute gastroenteritis. The present study was conducted to determine the clinical features and genetic diversity of HPeVs detected in children hospitalised for acute gastroenteritis. Stool specimens (n= 979) collected from children aged ⩽5 years hospitalised for acute gastroenteritis in Pune, western India during January 2006–December 2010 were included. HPeV RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) (5′UTR) followed by genotyping using VP1 gene-based PCR and phylogenetic analysis. HPeV was detected in 13·9% (136/979) of the cases, co-infections with other enteric viruses were found in 43·4%. HPeV was more frequent in children ⩽1 year age with infections reported throughout the year. A total of 102/136 (75%) HPeV strains were genotyped, which comprised 13 different HPeV genotypes. Of these, HPeV1 was the most predominant genotype detected and phylogenetically clustered with the Harris strain which is rarely reported. The study documents circulation of heterogeneous HPeV genotypes. Two variant strains of HPeV4 and ‘RGD absent’ HPeV5 and 6 strains were also detected. This is the first report of HPeV with diversified genotypes identified in acute gastroenteritis patients from India.

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Isolation and characterization of a rare group A rotavirus G13P[18] strain from a diarrhoeic foal in Japan

Journal of General Virology ◽

10.1099/jgv.0.001437 ◽

2020 ◽

Vol 101 (8) ◽

pp. 800-805

Author(s):

Manabu Nemoto ◽

Hidekazu Niwa ◽

Hiroshi Kida ◽

Tohru Higuchi ◽

Yasuhiro Orita ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Group A Rotavirus ◽

Isolation And Characterization ◽

Genotype Constellation ◽

Group A ◽

First Time ◽

Rare Group

A rare genotype G13P[18] group A rotavirus (RVA/Horse-tc/JPN/MK9/2019/G13P[18]) was isolated from a diarrhoeic foal for the first time in 28 years. The genotype constellation of the virus was assigned to G13-P[18]-I6-R9-C9-M6-A6-N9-T12-E14-H11 and was the same as that of the first isolated strain, RVA/Horse-tc/GBR/L338/1991/G13P[18]. Phylogenetic analysis suggests that the virus is related to RVA/Horse-tc/GBR/L338/1991/G13P[18] and is distant from typical equine rotaviruses of the G3P[12] and G14P[12] genotypes.

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ACUTE GASTROENTERITIS ASSOCIATED WITH NOROVIRUS GII.4 VARIANTS

Arquivos de Gastroenterologia ◽

10.1590/s0004-2803.201800000-67 ◽

2018 ◽

Vol 55 (3) ◽

pp. 264-266 ◽

Cited By ~ 3

Author(s):

Fabiana Lopes de PAULA ◽

Silvia Inês SARDI ◽

Dellane Martins TIGRE ◽

Flora Maria de Campos FERNANDES ◽

Gúbio Soares CAMPOS

Keyword(s):

Molecular Epidemiology ◽

New Orleans ◽

Acute Gastroenteritis ◽

Etiologic Agent ◽

Rt Pcr ◽

Stool Samples ◽

Sequences Analysis ◽

Norovirus Gii ◽

The City ◽

Children Under 5

ABSTRACT BACKGROUND: Norovirus (NoV) is an important etiologic agent of acute gastroenteritis and infects individuals of all ages, especially children in Brazil and worldwide. NoV GII.4 was the most prevalent genotype worldwide because of your extensive genetic diversity. In Brazil, especially in the Northeast, few studies have been developed for identify and molecularly characterize NoV. OBJECTIVE: The present study aimed to detect and describe the molecular epidemiology of NoV associated with acute gastroenteritis. METHODS: The viral RNA extracted from stool samples were subjected to Nested RT-PCR and the genotypes were determined by nucleotide sequences analysis. In total, 278 stool samples assisted at Aliança Hospital in the city of Salvador, with acute gastroenteritis were examined, between March 2009 and July 2012. RESULTS: A high NoV rate (54.2%) was identified in children under 5 years of age. We detected the circulation of different NoV GII.4 variants in Salvador, during the study period as Den Haag 2006b, New Orleans 2009 and Sydney 2012. CONCLUSION: These findings reinforce the need to study the molecular epidemiology of NoV infections in acute gastroenteritis.

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