scholarly journals Real-Time PCR Assay Using Fine-Needle Aspirates and Tissue Biopsy Specimens for Rapid Diagnosis of Mycobacterial Lymphadenitis in Children

2004 ◽  
Vol 42 (6) ◽  
pp. 2644-2650 ◽  
Author(s):  
E. S. Bruijnesteijn van Coppenraet ◽  
J. A. Lindeboom ◽  
J. M. Prins ◽  
M. F. Peeters ◽  
E. C. J. Claas ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Diagnosis ◽  
Pcr Assay ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Tissue Biopsy ◽  
Biopsy Specimens

scholarly journals Rapid diagnosis of human brucellosis by SYBR Green I-based real-time PCR assay and melting curve analysis in serum samples

2005 ◽  
Vol 11 (9) ◽  
pp. 713-718 ◽  
Author(s):  
M.I. Queipo-Ortuño ◽  
J.D. Colmenero ◽  
J.M. Reguera ◽  
M.A. García-Ordoñez ◽  
M.E. Pachón ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Rapid Diagnosis ◽  
Curve Analysis ◽  
Sybr Green I ◽  
Melting Curve Analysis ◽  
Human Brucellosis ◽  
Pcr Assay ◽  
Serum Samples

scholarly journals Development of duplex real-time PCR assay based on C18L and DNA pol genes for rapid diagnosis of camel pox in dromedary camels.

2021 ◽  
Vol 41 (1) ◽  
pp. 75-77
Author(s):  
Amir Shehata ◽  
Ehab ElNahas ◽  
Eman Abo Hatab ◽  
Saad Ali ◽  
Hanaa Ahmed
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Diagnosis ◽  
Dromedary Camels ◽  
Pcr Assay

Development of a multiplex real-time PCR assay for the rapid diagnosis of neonatal late onset sepsis

2014 ◽  
Vol 106 ◽  
pp. 8-15 ◽  
Author(s):  
Marre van den Brand ◽  
Remco P.H. Peters ◽  
Arnold Catsburg ◽  
Anna Rubenjan ◽  
Ferdi J. Broeke ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Late Onset ◽  
Rapid Diagnosis ◽  
Pcr Assay ◽  
Late Onset Sepsis

scholarly journals Quantitative Real-Time PCR Method for Detection of B-Lymphocyte Monoclonality by Comparison of κ and λ Immunoglobulin Light Chain Expression

2003 ◽  
Vol 49 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Anders Ståhlberg ◽  
Pierre Åman ◽  
Börje Ridell ◽  
Petter Mostad ◽  
Mikael Kubista
Keyword(s):  
B Cell ◽  
Real Time ◽  
Real Time Pcr ◽  
B Lymphocyte ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Clinical Samples ◽  
Quantitative Real Time Pcr ◽  
Fine Needle ◽  
Fine Needle Aspirates

Abstract Background: An abnormal IgLκ:IgLλ ratio has long been used as a clinical criterion for non-Hodgkin B-cell lymphomas. As a first step toward a quantitative real-time PCR-based multimarker diagnostic analysis of lymphomas, we have developed a method for determination of IgLκ:IgLλ ratio in clinical samples. Methods: Light-up probe-based real-time PCR was used to quantify IgLκ and IgLλ cDNA from 32 clinical samples. The samples were also investigated by routine immunohistochemical analysis and flow cytometry. Results: Of 32 suspected non-Hodgkin lymphoma samples analyzed, 28 were correctly assigned from real-time PCR measurements assuming invariant PCR efficiencies in the biological samples. Four samples were false negatives. One was a T-cell lymphoma, one was a diffuse large B-cell lymphoma, and one was reanalyzed and found lymphoma-positive by in situ calibration, which takes into account sample-specific PCR inhibition. Twelve of the samples were fine-needle aspirates, and these were all correctly assigned. Conclusions: This work is a first step toward analyzing clinical samples by quantitative light-up probe-based real-time PCR. Quantitative real-time PCR appears suitable for high-throughput testing of cancers by measuring expression of tumor markers in fine-needle aspirates.

scholarly journals Real-Time Automated PCR for Early Diagnosis and Monitoring of Cytomegalovirus Infection after Bone Marrow Transplantation

2000 ◽  
Vol 38 (7) ◽  
pp. 2536-2542 ◽  
Author(s):  
Utako Machida ◽  
Masahiro Kami ◽  
Takafumi Fukui ◽  
Yukumasa Kazuyama ◽  
Moritoshi Kinoshita ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Diagnosis ◽  
Qualitative Assessment ◽  
Cmv Infection ◽  
Pcr Assay ◽  
The Real ◽  
Antigenemia Assay ◽  
Blood Specimens ◽  
Interassay Precision

The purpose of this study was to assess the usefulness of real-time automated PCR as a quantitative, highly reproducible, and sensitive method to detect cytomegalovirus (CMV) DNA in blood specimens. Intra- and interassay precision rates were 0.89% (small number of copies [L]), 1.43% (middle number of copies [M]), and 1.12% (high number of copies [H]), and 4.46% (L), 1.51% (M), and 2.28% (H), respectively. The linearity of this assay was obtained between 10 and 107 copies/well, with a minimum detection limit of 20 copies/well. Specimens from 55 of 70 healthy subjects were found to be positive for CMV antibody, but CMV DNA was not detected in any of them. In the qualitative assessment of each specimen, the results of the CMV antigenemia assay and those of the real-time PCR assay agreed in 80% (plasma specimens), 79% (all nucleated cells), and 86% (blood) of the cases examined. For eight patients diagnosed as having CMV infection or disease, no sample was positive in the antigenemia assay earlier than in the real-time PCR assay. Furthermore, the results of this assay could be obtained within 8 h. We concluded that the real-time PCR assay is useful for rapid diagnosis of CMV infection and monitoring of clinical courses.

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