scholarly journals Evaluation of 12 Commercial Tests and the Complement Fixation Test for Mycoplasma pneumoniae-Specific Immunoglobulin G (IgG) and IgM Antibodies, with PCR Used as the "Gold Standard"

2005 ◽  
Vol 43 (5) ◽  
pp. 2277-2285 ◽  
Author(s):  
M. F. C. Beersma ◽  
K. Dirven ◽  
A. P. van Dam ◽  
K. E. Templeton ◽  
E. C. J. Claas ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Immunoglobulin G ◽  
Gold Standard ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Igm Antibodies ◽  
Specific Immunoglobulin

scholarly journals Development of Immunochromatographic Assay for the Rapid Detection of Mycoplasma capricolum subsp. capripneumoniae Antibodies

2022 ◽  
Vol 12 ◽  
Author(s):  
Zhen Zhu ◽  
Guanggang Qu ◽  
Changjiang Wang ◽  
Lei Wang ◽  
Jige Du ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Colloidal Gold ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Immunochromatographic Assay ◽  
Economic Losses ◽  
Serum Samples ◽  
Mycoplasma Capricolum ◽  
Prokaryotic Expression System

Mycoplasma capricolum subsp. capripneumoniae (Mccp) is the cause of contagious caprine pleuropneumonia (CCPP), which is a highly significant respiratory disease in goats leading to significant economic losses in Africa and Asia. Currently available procedures for the diagnosis of CCPP have some limitations in sensitivity, specificity, operation time, requirement of sophisticated equipment or skilled personnel, and cost. In this study, we developed a rapid, sensitive, and specific colloidal gold-based immunochromatographic assay (GICA) strip for the efficient on-site detection of antibodies against Mccp in the serum within 10 min. For the preparation of this colloidal GICA strip, recombinant P20 protein, the membrane protein of Mccp, was expressed by Escherichia coli prokaryotic expression system after purification was used as the binding antigen in the test. The rabbit anti-goat immunoglobulin G labeled with the colloidal gold was used as the detection probe, whereas the goat anti-rabbit immunoglobulin G was coated on the nitrocellulose membrane as the control line. The concentration of the coating antibody was optimized, and the effectiveness of this colloidal GICA strip was evaluated. Our results proved that the detection limit of the test strip was up to 1:64 dilutions for the Mccp antibody-positive serum samples with no cross-reactivity with other pathogens commonly infecting small ruminants,including goat pox virus, peste des petits ruminants virus, foot-and-mouth disease virus type A, or other mycoplasmas. Moreover, the colloidal GICA strip was more sensitive and specific than the indirect hemagglutination assay for the detection of Mccp antibodies. The 106 clinical serum samples were detected by the colloidal GICA strip compared with the complement fixation test, demonstrating an 87.74% concordance with the complement fixation test. This novel colloidal GICA strip would be an effective tool for the cost-effective and rapid diagnosis of CCPP in the field.

scholarly journals An epidemic ofMycoplasma pneumoniaein Manitoba: A Serological Report

1993 ◽  
Vol 4 (1) ◽  
pp. 43-46 ◽  
Author(s):  
Laila Sekla ◽  
Walter Stackiw ◽  
Gudrun Eibisch ◽  
Donna Kolton
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Respiratory Infections ◽  
Antibody Test ◽  
Fixation Test ◽  
Age Group ◽  
Serological Tests ◽  
Igm Antibodies ◽  
Indirect Immunofluorescent ◽  
Immunofluorescent Antibody Test

Objectives: To report an epidemic ofMycoplasma pneumoniaein Manitoba and to discuss the limitations of the serodiagnostic tests used.Design: A retrospective analysis of the results of a province-wide serological testing for respiratory infections caused byM pneumoniae,using a complement fixation test and an indirect immunofluorescent antibody test for the detection of immunoglobulin (Ig) M antibodies.Material: From April 1, 1987, to March 31, 1991, 12,804 sera were tested and a serological diagnosis of recentM pneumoniaeinfections were established in 509 (3.97%). From April 1 to September 30, 1991, an additional 2088 persons were tested; the 158 (7.5%) recent cases ofM pneumoniaewere subjected to analysis.Results: Compared with the previous three years, an increase in the number of recent cases ofM pneumoniaewas first noticed in July 1990 which persisted until September 1991. Of 856 single sera tested, 59 (6.8%) were recentM pneumoniaeinfections and 56 (96.1%) of these were positive for IgM antibodies. Of the 616 persons who submitted paired sera, 99 (16%) were recent infections, but only 46 (46.4%) had IgM antibodies. Primary infections (ie, positive for IgM antibodies) were detected in 102 (64.5%) and reinfections (ie, positive complement fixation test only) in the remaining 56 persons with recentM pneumoniaeinfections. Primary infections were detected more frequently in the ‘under 16’ than in the ‘over 16’ year age group (75% versus 55.8% of the recent cases ofM pneumoniaein each age group). Reinfections were more common in the older age group. Of the 158 recent cases ofM pneumoniae,30.3% had a pneumonia; of these, 21 (55.2%) were under the age of 16 years.Discussion:M pneumoniaeis an important cause of morbidity. Serological tests are used for the diagnosis despite their limitations. The detection of IgM antibodies in acute serum establishes a diagnosis of primaryM pneumoniae;however, their absence does not excludeM pneumoniae. Asecond (convalescent) blood test is required to diagnose all primary infections. To diagnose all reinfections, paired sera should be tested by complement fixation.Summary: Manitoba experienced an epidemic ofM pneumoniaein 1990–91. Properly selected serological tests can provide a specific and rapid diagnosis.

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