Development of Immunochromatographic Assay for the Rapid Detection of Mycoplasma capricolum subsp. capripneumoniae Antibodies

Mycoplasma capricolum subsp. capripneumoniae (Mccp) is the cause of contagious caprine pleuropneumonia (CCPP), which is a highly significant respiratory disease in goats leading to significant economic losses in Africa and Asia. Currently available procedures for the diagnosis of CCPP have some limitations in sensitivity, specificity, operation time, requirement of sophisticated equipment or skilled personnel, and cost. In this study, we developed a rapid, sensitive, and specific colloidal gold-based immunochromatographic assay (GICA) strip for the efficient on-site detection of antibodies against Mccp in the serum within 10 min. For the preparation of this colloidal GICA strip, recombinant P20 protein, the membrane protein of Mccp, was expressed by Escherichia coli prokaryotic expression system after purification was used as the binding antigen in the test. The rabbit anti-goat immunoglobulin G labeled with the colloidal gold was used as the detection probe, whereas the goat anti-rabbit immunoglobulin G was coated on the nitrocellulose membrane as the control line. The concentration of the coating antibody was optimized, and the effectiveness of this colloidal GICA strip was evaluated. Our results proved that the detection limit of the test strip was up to 1:64 dilutions for the Mccp antibody-positive serum samples with no cross-reactivity with other pathogens commonly infecting small ruminants,including goat pox virus, peste des petits ruminants virus, foot-and-mouth disease virus type A, or other mycoplasmas. Moreover, the colloidal GICA strip was more sensitive and specific than the indirect hemagglutination assay for the detection of Mccp antibodies. The 106 clinical serum samples were detected by the colloidal GICA strip compared with the complement fixation test, demonstrating an 87.74% concordance with the complement fixation test. This novel colloidal GICA strip would be an effective tool for the cost-effective and rapid diagnosis of CCPP in the field.

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Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2014-0052 ◽

2014 ◽

Vol 17 (2) ◽

pp. 367-369 ◽

Cited By ~ 3

Author(s):

K. Rypula ◽

A. Kumala ◽

P. Lis ◽

K. Niemczuk ◽

K. Płoneczka-Janeczko ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Rapid Detection ◽

Complement Fixation Test ◽

Complement Fixation ◽

Fixation Test ◽

Reproductive Disorders ◽

Serum Samples ◽

Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

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Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.8.1.119-122.2001 ◽

2001 ◽

Vol 8 (1) ◽

pp. 119-122 ◽

Cited By ~ 5

Author(s):

Rosanna Adone ◽

Franco Ciuchini

Keyword(s):

Immune Responses ◽

Brucella Abortus ◽

Complement Fixation Test ◽

Complement Fixation ◽

Infected Sheep ◽

Fixation Test ◽

Anticomplementary Activity ◽

Serum Samples ◽

Saline Extract ◽

Brucella Ovis

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.

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Seroprevalence of Coxiella burnetii in Polish cattle herds

Bulletin of the Veterinary Institute in Pulawy ◽

10.1515/bvip-2015-0071 ◽

2015 ◽

Vol 59 (4) ◽

pp. 479-482 ◽

Cited By ~ 3

Author(s):

Agnieszka Jodełko ◽

Krzysztof Niemczuk ◽

Monika Szymańska-Czerwińska

Keyword(s):

Coxiella Burnetii ◽

Complement Fixation Test ◽

Complement Fixation ◽

Fixation Test ◽

Serum Samples ◽

Average Percentage ◽

Cattle Herds

Abstract The aim of the study was to assess the seroprevalence of Coxiella burnetii in cattle herds in different regions of Poland. A total of 1150 serum samples collected from 443 cattle herds from 14 provinces were tested using complement fixation test. The seroprevalence was different in individual regions of Poland. The average percentage of seropositive herds was 40.41% and these herds were identified in each province tested.

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A study of human respiratory tract chlamydial infections in Cambridgeshire 1986–88

Epidemiology and Infection ◽

10.1017/s0950268800047488 ◽

1990 ◽

Vol 104 (3) ◽

pp. 479-488 ◽

Cited By ~ 9

Author(s):

T. G Wreghitt ◽

C. E Barker ◽

J. D Treharne ◽

J. M Phipps ◽

V Robinson ◽

...

Keyword(s):

Respiratory Tract ◽

Chlamydia Pneumoniae ◽

Complement Fixation Test ◽

Complement Fixation ◽

Chlamydia Psittaci ◽

Fixation Test ◽

Serum Samples ◽

Human Respiratory Tract ◽

Relative Incidence ◽

Chlamydial Infections

SUMMARYHuman respiratory tract chlamydial infections have been studied in Cambridge-shire for many years, but until recently we have been unable to distinguish between infection withChlamydia psittaciOrChlamydia pneumoniae(TWAR). In this study, we have employed the micro-immunofluorescence (micro-IF) test for this purpose and to look for the relative incidence ofC. psittaciandC. pneumoniaeinfections in Cambridgeshire. Among 50 patients with community-acquired respiratory tract symptoms whose serum samples had Chlamydia complement fixation test titres ≥ 64, 25 had evidence of recentC. psittaciorC. pneumoniaeinfection. Nineteen (76%) of the 25 patients had evidence of recentC. psittaciinfection and of these 16 (84%) had recently had contact with birds. Six patients (24%) had evidence of recentC. pneumoniaeinfection, and of these, only two (33% had recently had contact with birds). WhileC. psittaciwas grown from several of the birds associated with humanC. psittaciinfection, it was not cultured from any of the birds in contact with the two humanC. pnemoniaecases.

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Evaluation of 12 Commercial Tests and the Complement Fixation Test for Mycoplasma pneumoniae-Specific Immunoglobulin G (IgG) and IgM Antibodies, with PCR Used as the "Gold Standard"

Journal of Clinical Microbiology ◽

10.1128/jcm.43.5.2277-2285.2005 ◽

2005 ◽

Vol 43 (5) ◽

pp. 2277-2285 ◽

Cited By ~ 127

Author(s):

M. F. C. Beersma ◽

K. Dirven ◽

A. P. van Dam ◽

K. E. Templeton ◽

E. C. J. Claas ◽

...

Keyword(s):

Mycoplasma Pneumoniae ◽

Immunoglobulin G ◽

Gold Standard ◽

Complement Fixation Test ◽

Complement Fixation ◽

Fixation Test ◽

Igm Antibodies ◽

Specific Immunoglobulin

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Evaluation of the Complement Fixation Test in Amebiasis

Gastroenterology ◽

10.1016/s0016-5085(52)80038-1 ◽

1952 ◽

Vol 21 (3) ◽

pp. 391-399 ◽

Cited By ~ 1

Author(s):

Elwood Buchman ◽

Harold J. Kullman ◽

George F. Margonis

Keyword(s):

Complement Fixation Test ◽

Complement Fixation ◽

Fixation Test

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IMMUNOASSAY OF GONADOTROPHINS USING COMPLEMENT FIXATION

Acta Endocrinologica ◽

10.1530/acta.0.062s113 ◽

1969 ◽

Vol 62 (1_Suppl) ◽

pp. S113-S133 ◽

Cited By ~ 2

Author(s):

Sam Brody

Keyword(s):

Complement Fixation Test ◽

Complement Fixation ◽

Research Work ◽

Fixation Test ◽

Years Of Experience ◽

Standard Practice ◽

Clinical Observations ◽

Technical Procedure ◽

Methodological Problems

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.

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