scholarly journals Nucleotide sequence of the chi recombinational hot spot chi +D in bacteriophage lambda.

1981 ◽  
Vol 37 (1) ◽  
pp. 336-342 ◽  
Author(s):  
G R Smith ◽  
M Comb ◽  
D W Schultz ◽  
D L Daniels ◽  
F R Blattner
Keyword(s):  
Nucleotide Sequence ◽  
Hot Spot ◽  
Bacteriophage Lambda

scholarly journals Chromosomally Encoded blaCMY-2 Located on a Novel SXT/R391-Related Integrating Conjugative Element in a Proteus mirabilis Clinical Isolate

2010 ◽  
Vol 54 (9) ◽  
pp. 3545-3550 ◽  
Author(s):  
Sohei Harada ◽  
Yoshikazu Ishii ◽  
Tomoo Saga ◽  
Kazuhiro Tateda ◽  
Keizo Yamaguchi
Keyword(s):  
Nucleotide Sequence ◽  
Clinical Isolate ◽  
Proteus Mirabilis ◽  
Hot Spot ◽  
Gram Negative Bacteria ◽  
High Similarity ◽  
New Host ◽  
Gram Negative ◽  
Antimicrobial Resistance Genes ◽  
Serovar Typhimurium

ABSTRACT Integrating conjugative elements (ICEs) are mobile genetic elements that can transfer from the chromosome of a host to the chromosome of a new host through the process of excision, conjugation, and integration. Although SXT/R391-related ICEs, originally demonstrated in Vibrio cholerae O139 isolates, have become prevalent among V. cholerae isolates in Asia, the prevalence of the ICEs among Gram-negative bacteria other than Vibrio spp. remains unknown. In addition, SXT/R391-related ICEs carrying genes conferring resistance to extended-spectrum cephalosporins have never been described. Here we carried out a genetic analysis of a cefoxitin-resistant Proteus mirabilis clinical isolate, TUM4660, which revealed the presence of a novel SXT/R391-related ICE, ICEPmiJpn1. ICEPmiJpn1 had a core genetic structure showing high similarity to that of R391 and carried xis and int genes completely identical to those of R391, while an IS10-mediated composite transposon carrying bla CMY-2 was integrated into the ICE. A nucleotide sequence identical to the 3′ part of ISEcp1 was located upstream of the bla CMY-2 gene, and other genes observed around bla CMY-2 in earlier studies were also present. Furthermore, the nucleotide sequences of hot spot 2 and hot spot 4 in ICEPmiJpn1 showed high similarity to that of hot spot 2 in SXTMO10 and with a part of the nucleotide sequence found in P. mirabilis ATCC 29906, respectively. ICEPmiJpn1 was successfully transferred to Escherichia coli, Klebsiella pneumoniae, Salmonella enterica serovar Typhimurium, and Citrobacter koseri in conjugation experiments. These observations suggest that ICEs may contribute to the dissemination of antimicrobial resistance genes among clinically relevant Enterobacteriaceae, which warrants careful observation of the prevalence of ICEs, including SXT/R391-related ICEs.

scholarly journals Sequencing of FKS Hot Spot 1 from Saprochaete capitata To Search for a Relationship to Reduced Echinocandin Susceptibility

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
Inés Arrieta-Aguirre ◽  
Pilar Menéndez-Manjón ◽  
María Soledad Cuétara ◽  
Iñigo Fernández de Larrinoa ◽  
Juan Carlos García-Ruiz ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Fungal Pathogen ◽  
Hot Spot ◽  
Fungal Species ◽  
Catalytic Subunit ◽  
Glucan Synthase ◽  
Content Type ◽  
Oligonucleotide Primers ◽  
Geotrichum Capitatum ◽  
Flanking Regions

ABSTRACT Saprochaete capitata, formerly known as Geotrichum capitatum, is an emerging fungal pathogen with low susceptibility to echinocandins. Here, we report the nucleotide sequence of the S. capitata hot spot 1 region of the FKS gene (FKS HS1), which codifies for the catalytic subunit of β-1,3-d-glucan synthase, the target of echinocandins. For that purpose, we first designed degenerated oligonucleotide primers derived from conserved flanking regions of the FKS1 HS1 segment of 12 different fungal species. Interestingly, analysis of the translated FKS HS1 sequences of 12 isolates of S. capitata revealed that all of them exhibited the same F-to-L substitution in a position that is highly related to reduced echinocandin susceptibility.

scholarly journals Cloning of the groE operon of the marine bacterium Vibrio harveyi using a lambda vector.

1998 ◽  
Vol 45 (1) ◽  
pp. 261-270 ◽  
Author(s):  
D Kuchanny ◽  
G Klein ◽  
J Krzewska ◽  
A Czyz ◽  
B Lipińska
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Marine Bacterium ◽  
Southern Hybridization ◽  
Genomic Library ◽  
Vibrio Harveyi ◽  
Bacteriophage Lambda ◽  
E Coli ◽  
Escherichia Coli Cells ◽  
Dna Fragment

groES and groEL genes encode two co-operating proteins GroES and GroEL, belonging to a class of chaperone proteins highly conserved during evolution. The GroE chaperones are indispensable for the growth of bacteriophage lambda in Escherichia coli cells. In order to clone the groEL and groES genes of the marine bacterium Vibrio harveyi, we constructed the V. harveyi genomic library in the lambdaEMBL1 vector, and selected clones which were able to complement mutations in both groE genes of E. coli for bacteriophage lambda growth. Using Southern hybridization, in one of these clones we identified a DNA fragment homologous to the E. coli groE region. Analysis of the nucleotide sequence of this fragment showed that the cloned region contained a sequence in 71.7% homologous to the 3' end of the groEL gene of E. coli. This confirmed that the lambda clone indeed carries the groE region of V. harveyi. The positive result of our strategy of cloning with the use of the genomic library in lambda vector suggests that the same method might be useful in the isolation of the groE homologues from other bacteria. The V. harveyi cloned groE genes did not suppress thermosensitivity of the E. coli groE mutants.

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