scholarly journals Single-Cell Measurements of Fixation and Intercellular Exchange of C and N in the Filaments of the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120

mBio ◽  
2021 ◽  
Author(s):  
Mercedes Nieves-Morión ◽  
Enrique Flores ◽  
Martin J. Whitehouse ◽  
Aurélien Thomen ◽  
Rachel A. Foster
Keyword(s):  
Single Cell ◽  
Cellular Level ◽  
Model Organisms ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
C And N ◽  
Physiological Studies ◽  
Metabolic Activities ◽  
Intercellular Exchange ◽  
Pcc 7120

Filamentous, heterocyst-forming cyanobacteria represent a paradigm of multicellularity in the prokaryotic world. Physiological studies at the cellular level in model organisms are crucial to understand metabolic activities and qualify specific aspects related to multicellularity.

scholarly journals Biosensors-Based In Vivo Quantification of 2-Oxoglutarate in Cyanobacteria and Proteobacteria

Life ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 51 ◽  
Author(s):  
Hai-Lin Chen ◽  
Amel Latifi ◽  
Cheng-Cai Zhang ◽  
Christophe Bernard
Keyword(s):  
Conformational Changes ◽  
Nitrogen Assimilation ◽  
Krebs Cycle ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Cellular Level ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

2-oxoglutarate (α-ketoglutarate; 2-OG) is an intermediate of the Krebs cycle, and constitutes the carbon skeleton for nitrogen assimilation and the synthesis of a variety of compounds. In addition to being an important metabolite, 2-OG is a signaling molecule with a broad regulatory repertoire in a variety of organisms, including plants, animals, and bacteria. Although challenging, measuring the levels and variations of metabolic signals in vivo is critical to better understand how cells control specific processes. To measure cellular 2-OG concentrations and dynamics, we designed a set of biosensors based on the fluorescence resonance energy transfer (FRET) technology that can be used in vivo in different organisms. For this purpose, we took advantage of the conformational changes of two cyanobacterial proteins induced by 2-OG binding. We show that these biosensors responded immediately and specifically to different 2-OG levels, and hence allowed to measure 2-OG variations in function of environmental modifications in the proteobacterium Escherichia coli and in the cyanobacterium Anabaena sp. PCC 7120. Our results pave the way to study 2-OG dynamics at the cellular level in uni- and multi-cellular organisms.

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

Target Gene Inactivation in Cyanobacterium Anabaena sp. PCC 7120

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (15) ◽  
Author(s):  
Kangming Chen ◽  
Huilan Zhu ◽  
Liping Gu ◽  
Shengni Tian ◽  
Ruanbao Zhou
Keyword(s):  
Target Gene ◽  
Gene Inactivation ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

scholarly journals Spatio-Temporal Gene Induction Systems in the Heterocyst-Forming Multicellular Cyanobacterium Anabaena sp. PCC 7120

2017 ◽  
Vol 59 (1) ◽  
pp. 82-89 ◽  
Author(s):  
Akiyoshi Higo ◽  
Atsuko Isu ◽  
Yuki Fukaya ◽  
Toru Hisabori
Keyword(s):  
Gene Induction ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Spatio Temporal ◽  
Pcc 7120

scholarly journals Manipulation of Pattern of Cell Differentiation in a hetR Mutant of Anabaena sp. PCC 7120 by Overexpressing hetZ Alone or with hetP

Life ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 60 ◽  
Author(s):  
He Zhang ◽  
Xudong Xu
Keyword(s):  
Cell Differentiation ◽  
Peptide Inhibitor ◽  
Filamentous Cyanobacterium ◽  
Heterocyst Differentiation ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

In the filamentous cyanobacterium, Anabaena sp. PCC 7120, single heterocysts differentiate at semi-regular intervals in response to nitrogen stepdown. HetR is a principal regulator of heterocyst differentiation, and hetP and hetZ are two genes that are regulated directly by HetR. In a hetR mutant generated from the IHB (Institute of Hydrobiology) substrain of PCC 7120, heterocyst formation can be restored by moderate expression of hetZ and hetP. The resulting heterocysts are located at terminal positions. We used a tandem promoter, PrbcLPpetE, to express hetZ and hetP strongly in the hetR mutant. Co-expression of hetZ and hetP enabled the hetR mutant to form multiple contiguous heterocysts at both terminal and intercalary positions. Expression of hetZ, alone resulted in terminally located heterocysts, whereas expression of hetP, alone produced enlarged cells in strings. In the absence of HetR, formation of heterocysts was insensitive to the peptide inhibitor, RGSGR.

scholarly journals Different Functions of the Paralogs to the N-Terminal Domain of the Orange Carotenoid Protein in the Cyanobacterium Anabaena sp. PCC 7120

2016 ◽  
Vol 171 (3) ◽  
pp. 1852-1866 ◽  
Author(s):  
Rocío López-Igual ◽  
Adjélé Wilson ◽  
Ryan L. Leverenz ◽  
Matthew R. Melnicki ◽  
Céline Bourcier de Carbon ◽  
...  
Keyword(s):  
Terminal Domain ◽  
Cyanobacterium Anabaena ◽  
Orange Carotenoid Protein ◽  
Anabaena Sp ◽  
Pcc 7120

scholarly journals Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Vol 9 (1) ◽  
pp. 63-75 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn N. Wells ◽  
Arun J. Singh ◽  
Jessie Eiting ◽  
Philip J. Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Heterologous Expression ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120
Sign in / Sign up

Export Citation Format

Share Document