scholarly journals Release of two Saccharomyces cerevisiae cytochrome genes, COX6 and CYC1, from glucose repression requires the SNF1 and SSN6 gene products.

1990 ◽  
Vol 10 (3) ◽  
pp. 1297-1300 ◽  
Author(s):  
R M Wright ◽  
R O Poyton
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Genetic Background ◽  
Glucose Repression ◽  
Mitochondrial Proteins ◽  
Gene Products ◽  
Oxidase Subunit ◽  
Yeast Genes

We show here that SNF1 and SSN6 are required for derepression of the glucose-repressible yeast genes COX6 and CYC1, which encode the mitochondrial proteins cytochrome c oxidase subunit VI and iso-1-cytochrome c, respectively. In an snf1 mutant genetic background, the transcription of both COX6 and CYC1 continued to be repressed after cells were shifted into derepressing media. In an ssn6 mutant genetic background, both COX6 and CYC1 were expressed constitutively at high levels in repressing media. SSN6 acted epistatically to SNF1 in the regulation of both cytochrome genes. These findings are similar to previous findings on the effects of SNF1 and SSN6 on SUC2 expression in Saccharomyces cerevisiae and are consistent with a model proposing that SNF1 exerts its effect through SSN6 on COX6 and CYC1.

Release of two Saccharomyces cerevisiae cytochrome genes, COX6 and CYC1, from glucose repression requires the SNF1 and SSN6 gene products

1990 ◽  
Vol 10 (3) ◽  
pp. 1297-1300
Author(s):  
R M Wright ◽  
R O Poyton
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Genetic Background ◽  
Glucose Repression ◽  
Mitochondrial Proteins ◽  
Gene Products ◽  
Oxidase Subunit ◽  
Yeast Genes

We show here that SNF1 and SSN6 are required for derepression of the glucose-repressible yeast genes COX6 and CYC1, which encode the mitochondrial proteins cytochrome c oxidase subunit VI and iso-1-cytochrome c, respectively. In an snf1 mutant genetic background, the transcription of both COX6 and CYC1 continued to be repressed after cells were shifted into derepressing media. In an ssn6 mutant genetic background, both COX6 and CYC1 were expressed constitutively at high levels in repressing media. SSN6 acted epistatically to SNF1 in the regulation of both cytochrome genes. These findings are similar to previous findings on the effects of SNF1 and SSN6 on SUC2 expression in Saccharomyces cerevisiae and are consistent with a model proposing that SNF1 exerts its effect through SSN6 on COX6 and CYC1.

scholarly journals Translocation and Assembly of Mitochondrially Coded Saccharomyces cerevisiae Cytochrome c Oxidase Subunit Cox2 by Oxa1 and Yme1 in the Absence of Cox18

Genetics ◽  
2009 ◽  
Vol 182 (2) ◽  
pp. 519-528 ◽  
Author(s):  
Heather L. Fiumera ◽  
Maitreya J. Dunham ◽  
Scott A. Saracco ◽  
Christine A. Butler ◽  
Jessica A. Kelly ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxidase Subunit

Oxygen Regulation of the Cytochrome c Oxidase Subunit VI Gene, COX6, in Saccharomyces cerevisiae

1995 ◽  
Vol 216 (2) ◽  
pp. 676-685 ◽  
Author(s):  
R.M. Wright ◽  
S.L. Simpson ◽  
B.D. Lanoil
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxygen Regulation ◽  
Oxidase Subunit

Neither respiration nor cytochrome c oxidase affects mitochondrial morphology in Saccharomyces cerevisiae.

1998 ◽  
Vol 201 (11) ◽  
pp. 1729-1737 ◽  
Author(s):  
C Church ◽  
R O Poyton
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Respiratory Chain ◽  
Glucose Repression ◽  
Nuclear Gene ◽  
Yeast Cells ◽  
Mitochondrial Proteins ◽  
Mitochondrial Morphology ◽  
Per Se ◽  
Mitochondrial Reticulum

Previous studies have reported that mitochondrial morphology and volume in yeast cells are linked to cellular respiratory capacity. These studies revealed that mitochondrial morphology in glucose-repressed or anaerobically grown cells, which lack or have reduced levels of respiration, is different from that in fully respiring cells. Although both oxygen deprivation and glucose repression decrease the levels of respiratory chain proteins, they decrease the expression of many non-mitochondrial proteins as well, making it difficult to determine whether it is a defect in respiration or something else that effects mitochondrial morphology. To determine whether mitochondrial morphology is dependent on respiration per se, we used a strain with a null mutation in PET100, a nuclear gene that is specifically required for the assembly of cytochrome c oxidase. Although this strain lacks respiration, the mitochondrial morphology and volumes are both comparable to those found in its respiration-proficient parent. These findings indicate that respiration is not involved in the establishment or maintenance of yeast mitochondrial morphology, and that the previously observed effects of oxygen availability and glucose repression on mitochondrial morphology are not exerted through the respiratory chain. By applying the principle of symmorphosis to these findings, we conclude that the shape and size of the mitochondrial reticulum found in respiring yeast cells is maintained for reasons other than respiration.

Insight into topological and functional relationships of cytochrome c oxidase subunit I of Saccharomyces cerevisiae by means of intragenic complementation

FEBS Letters ◽  
1993 ◽  
Vol 321 (2-3) ◽  
pp. 159-162 ◽  
Author(s):  
Brigitte Meunier ◽  
Françoise Coster ◽  
Philippe Lemarre ◽  
Anne-Marie Colson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Functional Relationships ◽  
Oxidase Subunit ◽  
Intragenic Complementation ◽  
Insight Into

scholarly journals Overexpression of a leaderless form of yeast cytochrome c oxidase subunit Va circumvents the requirement for a leader peptide in mitochondrial import.

1990 ◽  
Vol 10 (9) ◽  
pp. 4984-4986 ◽  
Author(s):  
L K Dircks ◽  
R O Poyton
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Mitochondrial Protein ◽  
Leader Peptide ◽  
Mitochondrial Import ◽  
Oxidase Subunit

Subunit Va of Saccharomyces cerevisiae cytochrome c oxidase is a nucleus-encoded mitochondrial protein that is derived from a precursor with a 20-residue leader peptide. We previously reported that this leader peptide is required for import of subunit Va into mitochondria in vivo (S. M. Glaser, C. E. Trueblood, L. K. Dircks, R. O. Poyton, and M. G. Cumsky, J. Cell. Biochem. 36:275-278, 1988). Here we show that overproduction of a leaderless form of subunit Va circumvents the leader peptide requirement for import into mitochondria in vivo.

Mss51p, a putative translational activator of cytochrome c oxidase subunit-1 ( COX1 ) mRNA, is required for synthesis of Cox1p in Saccharomyces cerevisiae

2000 ◽  
Vol 37 (4) ◽  
pp. 213-220 ◽  
Author(s):  
M. Siep ◽  
K. van Oosterum ◽  
H. Neufeglise ◽  
H. van der Spek ◽  
L. A. Grivell
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxidase Subunit ◽  
Translational Activator
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