Oxidant stress leads to transcriptional activation of the human heme oxygenase gene in cultured skin fibroblasts

Treatment of cultured human skin fibroblasts with near-UV radiation, hydrogen peroxide, and sodium arsenite induces accumulation of heme oxygenase mRNA and protein. In this study, these treatments led to a dramatic increase in the rate of RNA transcription from the heme oxygenase gene but had no effect on mRNA stability. Transcriptional activation, therefore, appears to be the major mechanism of stimulation of expression of this gene by either oxidative stress or sulfydryl reagents.

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Oxidant stress leads to transcriptional activation of the human heme oxygenase gene in cultured skin fibroblasts.

Molecular and Cellular Biology ◽

10.1128/mcb.10.9.4967 ◽

1990 ◽

Vol 10 (9) ◽

pp. 4967-4969 ◽

Cited By ~ 159

Author(s):

S M Keyse ◽

L A Applegate ◽

Y Tromvoukis ◽

R M Tyrrell

Keyword(s):

Heme Oxygenase ◽

Transcriptional Activation ◽

Mrna Stability ◽

Sodium Arsenite ◽

Oxidant Stress ◽

Skin Fibroblasts ◽

Rna Transcription ◽

Major Mechanism ◽

Cultured Skin ◽

Stimulation Of

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TWO GENES CONTRIBUTE TO DIFFERENT EXTENTS TO THE HEME OXYGENASE ENZYME ACTIVITY MEASURED IN CULTURED HUMAN SKIN FIBROBLASTS AND KERATINOCYTES: IMPLICATIONS FOR PROTECTION AGAINST OXIDANT STRESS

Photochemistry and Photobiology ◽

10.1111/j.1751-1097.1995.tb03973.x ◽

1995 ◽

Vol 61 (3) ◽

pp. 285-291 ◽

Cited By ~ 84

Author(s):

Lee Ann Applegate ◽

Alexandre Noël ◽

Glenn Vile ◽

Edgar Frenk ◽

Rex M. Tyrrell

Keyword(s):

Enzyme Activity ◽

Human Skin ◽

Heme Oxygenase ◽

Oxidant Stress ◽

Skin Fibroblasts ◽

Human Skin Fibroblasts

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Stimulation of fatty acid uptake and triglyceride synthesis in human cultured skin fibroblasts and adipocytes by a serum protein

Biochemical and Biophysical Research Communications ◽

10.1016/s0006-291x(87)80480-1 ◽

1987 ◽

Vol 144 (1) ◽

pp. 94-100 ◽

Cited By ~ 12

Author(s):

K. Cianflone ◽

P.O. Kwiterovich ◽

M. Walsh ◽

A. Forse ◽

M.A. Rodriguez ◽

...

Keyword(s):

Fatty Acid ◽

Serum Protein ◽

Fatty Acid Uptake ◽

Skin Fibroblasts ◽

Acid Uptake ◽

Triglyceride Synthesis ◽

Cultured Skin ◽

Stimulation Of

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Heme oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide, and sodium arsenite.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.86.1.99 ◽

1989 ◽

Vol 86 (1) ◽

pp. 99-103 ◽

Cited By ~ 884

Author(s):

S. M. Keyse ◽

R. M. Tyrrell

Keyword(s):

Hydrogen Peroxide ◽

Human Skin ◽

Heme Oxygenase ◽

Sodium Arsenite ◽

Stress Protein ◽

Skin Fibroblasts ◽

Human Skin Fibroblasts ◽

Uva Radiation

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Electron Microscopy of Fibroblasts from Myotonic Muscular Dystrophy Patients

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100098976 ◽

1981 ◽

Vol 39 ◽

pp. 498-499

Author(s):

S. E. Miller ◽

G. B. Hartwig ◽

R. A. Nielsen ◽

A. P. Frost ◽

A. D. Roses

Keyword(s):

Electron Microscopy ◽

Muscular Dystrophy ◽

Genetic Diseases ◽

Skin Fibroblasts ◽

Inborn Errors ◽

Cytoplasmic Inclusions ◽

Cultured Skin ◽

Myotonic Muscular Dystrophy ◽

Glycosaminoglycan Metabolism

Many genetic diseases can be demonstrated in skin cells cultured in vitro from patients with inborn errors of metabolism. Since myotonic muscular dystrophy (MMD) affects many organs other than muscle, it seems likely that this defect also might be expressed in fibroblasts. Detection of an alteration in cultured skin fibroblasts from patients would provide a valuable tool in the study of the disease as it would present a readily accessible and controllable system for examination. Furthermore, fibroblast expression would allow diagnosis of fetal and presumptomatic cases. An unusual staining pattern of MMD cultured skin fibroblasts as seen by light microscopy, namely, an increase in alcianophilia and metachromasia, has been reported; both these techniques suggest an altered glycosaminoglycan metabolism An altered growth pattern has also been described. One reference on cultured skin fibroblasts from a different dystrophy (Duchenne Muscular Dystrophy) reports increased cytoplasmic inclusions seen by electron microscopy. Also, ultrastructural alterations have been reported in muscle and thalamus biopsies from MMD patients, but no electron microscopical data is available on MMD cultured skin fibroblasts.

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