scholarly journals HMG1-related DNA-binding protein isolated with V-(D)-J recombination signal probes.

1991 ◽  
Vol 11 (9) ◽  
pp. 4528-4536 ◽  
Author(s):  
M Shirakata ◽  
K Hüppi ◽  
S Usuda ◽  
K Okazaki ◽  
K Yoshida ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cdna Clones ◽  
Chromosomal Protein ◽  
Chromosome 2 ◽  
Recombination Signal ◽  
Length Polymorphisms ◽  
Mouse Chromosome 2 ◽  
Rag 1 ◽  
Phage Libraries ◽  
Radiolabeled Probe

In order to isolate cDNA clones for DNA-binding components of the V-(D)-J recombinase, phage libraries from a pre-B-cell line were screened with a radiolabeled probe containing recombination signal sequences (RSS). Among prospective clones, cDNA T160 was analyzed further. It produced a protein of 80.6 kDa which bound to DNA containing RSS but not to DNA in which the RSS had been mutated. A search of a data base revealed that the T160 protein has significant sequence homology (56%) to the nonhistone chromosomal protein HMG1 within the C-terminal region of 80 amino acids. DNA-binding analysis with truncated proteins showed that the HMG homology region is responsible for DNA binding. Using restriction fragment length polymorphisms, the T160 gene was mapped at the proximal end of mouse chromosome 2. Evidence was obtained for genetic linkage between the T160 gene and the recombination activator genes RAG-1 and RAG-2.

HMG1-related DNA-binding protein isolated with V-(D)-J recombination signal probes

1991 ◽  
Vol 11 (9) ◽  
pp. 4528-4536
Author(s):  
M Shirakata ◽  
K Hüppi ◽  
S Usuda ◽  
K Okazaki ◽  
K Yoshida ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cdna Clones ◽  
Chromosomal Protein ◽  
Chromosome 2 ◽  
Recombination Signal ◽  
Length Polymorphisms ◽  
Mouse Chromosome 2 ◽  
Rag 1 ◽  
Phage Libraries ◽  
Radiolabeled Probe

In order to isolate cDNA clones for DNA-binding components of the V-(D)-J recombinase, phage libraries from a pre-B-cell line were screened with a radiolabeled probe containing recombination signal sequences (RSS). Among prospective clones, cDNA T160 was analyzed further. It produced a protein of 80.6 kDa which bound to DNA containing RSS but not to DNA in which the RSS had been mutated. A search of a data base revealed that the T160 protein has significant sequence homology (56%) to the nonhistone chromosomal protein HMG1 within the C-terminal region of 80 amino acids. DNA-binding analysis with truncated proteins showed that the HMG homology region is responsible for DNA binding. Using restriction fragment length polymorphisms, the T160 gene was mapped at the proximal end of mouse chromosome 2. Evidence was obtained for genetic linkage between the T160 gene and the recombination activator genes RAG-1 and RAG-2.

scholarly journals Identification and expression of the cDNA of KIN17, a zinc-finger gene located on mouse chromosome 2, encoding a new DNA-binding protein

1991 ◽  
Vol 19 (19) ◽  
pp. 5117-5123 ◽  
Author(s):  
Jaime F. Angulo ◽  
Evelyne Rouer ◽  
Alexander Mazin ◽  
Marie-geneviève Mattei ◽  
Agnèves Tissier ◽  
...  
Keyword(s):  
Dna Binding ◽  
Mouse Chromosome ◽  
Zinc Finger ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Chromosome 2 ◽  
Zinc Finger Gene ◽  
Mouse Chromosome 2

scholarly journals Recombinant inbred strain and interspecific backcross analysis of molecular markers flanking the murine agouti coat color locus.

Genetics ◽  
1989 ◽  
Vol 122 (3) ◽  
pp. 669-679
Author(s):  
L D Siracusa ◽  
A M Buchberg ◽  
N G Copeland ◽  
N A Jenkins
Keyword(s):  
Molecular Markers ◽  
Inbred Strain ◽  
Mouse Chromosome ◽  
Recombinant Inbred ◽  
Recombinant Inbred Strain ◽  
Interspecific Backcross ◽  
Chromosome 2 ◽  
Chromosome 4 ◽  
Agouti Locus ◽  
Mouse Chromosome 2

Abstract Recombinant inbred strain and interspecific backcross mice were used to create a molecular genetic linkage map of the distal portion of mouse chromosome 2. The orientation and distance of the Ada, Emv-13, Emv-15, Hck-1, Il-1a, Pck-1, Psp, Src-1 and Svp-1 loci from the beta 2-microglobulin locus and the agouti locus were established. Our mapping results have provided the identification of molecular markers both proximal and distal to the agouti locus. The recombinants obtained provide valuable resources for determining the direction of chromosome walking experiments designed to clone sequences at the agouti locus. Comparisons between the mouse and human genome maps suggest that the human homolog of the agouti locus resides on human chromosome 20q. Three loci not present on mouse chromosome 2 were also identified and were provisionally named Psp-2, Hck-2 and Hck-3. The Psp-2 locus maps to mouse chromosome 14. The Hck-2 locus maps near the centromere of mouse chromosome 4 and may identify the Lyn locus. The Hck-3 locus maps near the distal end of mouse chromosome 4 and may identify the Lck locus.

Evidence for two commonly deleted regions on mouse chromosome 2 in γ ray–induced acute myeloid leukemic cells

2002 ◽  
Vol 30 (6) ◽  
pp. 564-570 ◽  
Author(s):  
Kanokporn Rithidech ◽  
John J Dunn ◽  
Bruce A Roe ◽  
Chris R Gordon ◽  
Eugene P Cronkite
Keyword(s):  
Mouse Chromosome ◽  
Leukemic Cells ◽  
Chromosome 2 ◽  
Mouse Chromosome 2 ◽  
Γ Ray ◽  
Acute Myeloid

Functionally distinct isoforms of the CRE-BP DNA-binding protein mediate activity of a T-cell-specific enhancer

1992 ◽  
Vol 12 (2) ◽  
pp. 747-757
Author(s):  
K Georgopoulos ◽  
B A Morgan ◽  
D D Moore
Keyword(s):  
T Cell ◽  
Dna Binding ◽  
Cyclic Amp ◽  
Protein A ◽  
Cell Receptor ◽  
Protein Isoforms ◽  
Cdna Clones ◽  
C Terminus ◽  
Transcription Regulators ◽  
Cyclic Amp Response Element

Expression of the CD3 delta gene of the T-cell receptor (TCR) complex is regulated by a T-cell-specific enhancer. A highly conserved 40-bp motif (element delta A) within the CD3 delta enhancer is responsible for mediating its activity and specificity. Element delta A exhibits sequence similarities to the cyclic AMP response element (CRE) but does not respond to changes in the level of cyclic AMP. Using the delta A element as a probe, we have isolated three cDNA clones encoding three distinct protein isoforms, products of differential splicing and alternate promoter usage of the CRE-BP gene. These isoforms share the DNA binding and dimerization domains at the C terminus of the protein but differ at their N termini. In transfection assays, their activities as transcription regulators differ: CRE-BP2 is a potent activator, CRE-BP3 is a weak activator, and CRE-BP1 is transcriptionally inert. Mutations in the basic region of the CRE-BP1 protein which abrogate its ability to bind DNA render this protein a dominant repressor of the delta A enhancer. Antibodies to the CRE-BP protein interact specifically with the ubiquitous and predominantly T-cell-restricted nuclear complexes that bind to the delta A element and suggest the presence of this protein in homo- and heterodimeric complexes. Since the delta A motif is also present in the enhancer and promoter of the TCR alpha and beta genes, the CRE-BP isoforms may mediate expression of other members of the CD3/TCR complex during T-cell development.

Mapping of the structural gene for S-adenosyl homocysteine hydrolase to mouse Chromosome 2, and related sequences to Chromosomes 8 and X

1992 ◽  
Vol 3 (11) ◽  
pp. 633-636 ◽  
Author(s):  
Alison Pilz ◽  
Paul Le Tissier ◽  
Heather Moseley ◽  
Jo Peters ◽  
Cathy Abbott
Keyword(s):  
Mouse Chromosome ◽  
Structural Gene ◽  
Chromosome 2 ◽  
Mouse Chromosome 2

scholarly journals Subunit of an alpha-interferon-responsive transcription factor is related to interferon regulatory factor and Myb families of DNA-binding proteins.

1992 ◽  
Vol 12 (8) ◽  
pp. 3315-3324 ◽  
Author(s):  
S A Veals ◽  
C Schindler ◽  
D Leonard ◽  
X Y Fu ◽  
R Aebersold ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Proteins ◽  
Dna Recognition ◽  
Interferon Regulatory Factor ◽  
Dna Binding Proteins ◽  
Alpha Interferon ◽  
Cdna Clones ◽  
Regulatory Factor ◽  
Amino Terminal ◽  
Sequence Similarities

Alpha interferon stimulates transcription by converting the positive transcriptional regulator ISGF3 from a latent to an active form. This receptor-mediated event occurs in the cytoplasm, with subsequent translocation of the activated factor to the nucleus. ISGF3 has two components, termed ISGF3 alpha and ISGF3 gamma. ISGF3 gamma serves as the DNA recognition subunit, while ISGF3 alpha, which appears to consist of three polypeptides, is a target for alpha interferon signaling and serves as a regulatory component whose activation is required to form ISGF3. ISGF3 gamma DNA-binding activity was identified as a 48-kDa polypeptide, and partial amino acid sequence has allowed isolation of cDNA clones. ISGF3 gamma translated in vitro from recombinant clones bound DNA with a specificity indistinguishable from that of ISGF3 gamma purified from HeLa cells. Sequencing of ISGF3 gamma cDNA clones revealed significant similarity to the interferon regulatory factor (IRF) family of DNA binding proteins in the amino-terminal 117 residues of ISGF3 gamma. The other IRF family proteins bind DNA with a specificity related to but distinct from that of ISGF3 gamma. We note sequence similarities between the related regions of IRF family proteins and the imperfect tryptophan repeats which constitute the DNA-binding domain of the c-myb oncoprotein. These sequence similarities suggest that ISGF3 gamma and IRF proteins and the c-myb oncoprotein use a common structural motif for DNA recognition. Recombinant ISGF3 gamma, like the natural protein, interacted with HeLa cell ISGF3 alpha to form the mature ISGF3 DNA-binding complex. We suggest that other IRF family members may participate in signaling pathways by interacting with as yet unidentified regulatory subunits analogous to ISGF3 alpha.

Mouse chromosome 2

1998 ◽  
Vol 8 (S1) ◽  
pp. S27-S49 ◽  
Author(s):  
Josephine Peters ◽  
Linda D. Siracusa ◽  
Daniel Pomp ◽  
Aamir R. Zuberi ◽  
Deanna Church ◽  
...  
Keyword(s):  
Mouse Chromosome ◽  
Chromosome 2 ◽  
Mouse Chromosome 2
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