scholarly journals Complete Genome Sequence of an Extremely Halophilic Archaeon from Great Salt Lake, Halobacterium sp. GSL-19

2021 ◽  
Vol 10 (28) ◽  
Author(s):  
Priya DasSarma ◽  
Brian P. Anton ◽  
Hedvig A. L. von Ehrenheim ◽  
Richard J. Roberts ◽  
Shiladitya DasSarma
Keyword(s):  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Salt Lake ◽  
Great Salt Lake ◽  
Halophilic Archaeon ◽  
Smrt Sequencing ◽  
Circular Chromosome ◽  
The North ◽  
Extremely Halophilic Archaeon

An extremely halophilic archaeon, Halobacterium sp. strain GSL-19, was isolated from the north arm of Great Salt Lake in Utah. Single-molecule real-time (SMRT) sequencing was used to establish a GC-rich 2.3-Mbp genome composed of a circular chromosome and 2 plasmids, with 2,367 predicted genes, including 1 coding a CTAG-methylase widely distributed among Haloarchaea .

scholarly journals Genome Sequence of Salarchaeum sp. Strain JOR-1, an Extremely Halophilic Archaeon from the Dead Sea

2020 ◽  
Vol 9 (5) ◽  
Author(s):  
Brian P. Anton ◽  
Priya DasSarma ◽  
Fabiana L. Martinez ◽  
Satyajit L. DasSarma ◽  
Mohammad Al Madadha ◽  
...  
Keyword(s):  
Real Time ◽  
Genome Sequence ◽  
Single Molecule ◽  
East Coast ◽  
Dead Sea ◽  
Halophilic Archaeon ◽  
Smrt Sequencing ◽  
Circular Chromosome ◽  
Extremely Halophilic Archaeon ◽  
The Dead

An extremely halophilic archaeon, Salarchaeum sp. strain JOR-1, was isolated from the east coast of the Dead Sea, Kingdom of Jordan, and sequenced using single-molecule real-time (SMRT) sequencing. The GC-rich 2.5-Mbp genome was composed of a circular chromosome and a megaplasmid. The genome contained 2,633 genes and was incorporated into HaloWeb (https://halo.umbc.edu/).

scholarly journals Complete Genome Sequence of Agrococcus sp. Strain SGAir0287, Isolated from Tropical Air Collected in Singapore

2019 ◽  
Vol 8 (32) ◽  
Author(s):  
Kenny J. X. Lau ◽  
Ana Carolina M. Junqueira ◽  
Akira Uchida ◽  
Rikky W. Purbojati ◽  
James N. I. Houghton ◽  
...  
Keyword(s):  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Smrt Sequencing ◽  
Entire Genome ◽  
Circular Chromosome ◽  
Protein Coding ◽  
Content Type ◽  
Air Samples ◽  
Protein Coding Genes

Agrococcus sp. strain SGAir0287 was isolated from tropical air samples collected in Singapore. Assembled using single-molecule real-time (SMRT) sequencing and MiSeq reads, the genome consists of one circular chromosome of 3,084,767 bp. The entire genome has 2,870 protein-coding genes, 45 tRNAs, and 3 rRNAs.

scholarly journals Complete Genome Sequence and Methylome Analysis of Acinetobacter calcoaceticus 65

2017 ◽  
Vol 5 (12) ◽  
Author(s):  
Alexey Fomenkov ◽  
Tamas Vincze ◽  
Sergey K. Degtyarev ◽  
Richard J. Roberts
Keyword(s):  
Single Molecule ◽  
Restriction Enzyme ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Acinetobacter Calcoaceticus ◽  
Complete Sequence ◽  
Smrt Sequencing ◽  
Original Source ◽  
Content Type ◽  
Methylome Analysis

ABSTRACT Acinetobacter calcoaceticus 65 is the original source strain for the restriction enzyme Acc65I. Its complete sequence and full methylome were determined using single-molecule real-time (SMRT) sequencing.

scholarly journals Complete Genome Sequence of Brevundimonas sp. Strain SGAir0440, Isolated from Indoor Air in Singapore

2019 ◽  
Vol 8 (31) ◽  
Author(s):  
Rikky W. Purbojati ◽  
Daniela I. Drautz-Moses ◽  
Akira Uchida ◽  
Anthony Wong ◽  
Megan E. Clare ◽  
...  
Keyword(s):  
Single Molecule ◽  
Indoor Air ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Sequencing Data ◽  
Circular Chromosome ◽  
Protein Coding ◽  
Content Type ◽  
Air Samples ◽  
Protein Coding Genes

Brevundimonas sp. strain SGAir0440 was isolated from indoor air samples collected in Singapore. Its genome was assembled using single-molecule real-time sequencing data, resulting in one circular chromosome with a length of 3.1 Mbp. The genome consists of 3,033 protein-coding genes, 48 tRNAs, and 6 rRNA operons.

scholarly journals Complete Genome and Methylome Analysis of the Box-Shaped Halophilic Archaeon Haloarcula sinaiiensis ATCC 33800

2021 ◽  
Vol 10 (34) ◽  
Author(s):  
Alexey Fomenkov ◽  
Priya DasSarma ◽  
Sean P. Kennedy ◽  
Richard J. Roberts ◽  
Shiladitya DasSarma
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Complete Genome ◽  
Bioinformatic Analysis ◽  
Halophilic Archaeon ◽  
Smrt Sequencing ◽  
Content Type ◽  
Methylome Analysis

The genome of halophilic archaeon Haloarcula sinaiiensis ATCC 33800 was sequenced and assembled and comprises seven replicons. Four m6A and one m4C modified motifs and their responsible methyltransferase genes have been identified in the genome by single-molecule real-time (SMRT) sequencing and bioinformatic analysis.

scholarly journals Complete Genome Sequence and Methylome Analysis ofBacillus globigiiATCC 49760

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Richard D. Morgan
Keyword(s):  
Bacillus Subtilis ◽  
Real Time ◽  
Genome Sequence ◽  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Restriction Enzymes ◽  
Complete Sequence ◽  
Smrt Sequencing ◽  
Methylome Analysis

Bacillus subtilis(Ehrenburg) Cohn ATCC 49760, deposited asBacillus globigii, is the source strain for the restriction enzymes BglI and BglII. Its complete sequence and full methylome were determined using single-molecule real-time (SMRT) sequencing.

scholarly journals Complete Genome Sequence of Acidithiobacillus ferridurans JAGS, Isolated from Acidic Mine Drainage

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
Jinjin Chen ◽  
Yilan Liu ◽  
Patrick Diep ◽  
Andrew Jo ◽  
Camilla Nesbø ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Mine Drainage ◽  
Gc Content ◽  
Acidic Mine Drainage ◽  
Smrt Sequencing ◽  
Protein Coding ◽  
Content Type

We report a complete genome sequence of Acidithiobacillus ferridurans JAGS, determined using PacBio single-molecule real-time (SMRT) sequencing. The circular genome of JAGS (2,933,811 bp; GC content, 58.57%) contains 3,001 protein-coding sequences, 46 tRNAs, and 6 rRNAs. Predicted genes indicate the potential to fix CO2 and N2 and to utilize Fe2+, S0, and H2 as energy sources.

scholarly journals Complete Genome Sequence of Bartonella alsatica Strain IBS 382 (CIP 105477)

2020 ◽  
Vol 9 (34) ◽  
Author(s):  
Arno Thibau ◽  
Tilman G. Schultze ◽  
Wibke Ballhorn ◽  
Volkhard A. J. Kempf
Keyword(s):  
Genome Sequence ◽  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Zoonotic Potential ◽  
Smrt Sequencing ◽  
Content Type ◽  
Long Read ◽  
Human Infections ◽  
Future Work

ABSTRACT Bartonella alsatica causes bacteremia in rabbits and, rarely, human infections. Here, we announce the complete and closed genome of B. alsatica IBS 382 (CIP 105477), generated by long-read Pacific Biosciences single-molecule real-time (SMRT) sequencing. The availability of this genome sequence allows future work on understanding the zoonotic potential of this pathogen.

scholarly journals Complete Genome Sequence of Pseudomonas aeruginosa Phage-Resistant Variant PA1RG

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Gang Li ◽  
Shuguang Lu ◽  
Mengyu Shen ◽  
Shuai Le ◽  
Yinling Tan ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Genome Sequence ◽  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
De Novo ◽  
Smrt Sequencing ◽  
Sequence Coverage ◽  
Resistant Variant ◽  
Defense Systems

Bacteria have evolved several defense systems against phage predation. Here, we report the 6,500,439-bp complete genome sequence of the Pseudomonas aeruginosa phage-resistant variant PA1RG. Single-molecule real-time (SMRT) sequencing and de novo assembly revealed a single contig with 320-fold sequence coverage.

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