scholarly journals Genome Sequence of Salarchaeum sp. Strain JOR-1, an Extremely Halophilic Archaeon from the Dead Sea

2020 ◽  
Vol 9 (5) ◽  
Author(s):  
Brian P. Anton ◽  
Priya DasSarma ◽  
Fabiana L. Martinez ◽  
Satyajit L. DasSarma ◽  
Mohammad Al Madadha ◽  
...  

An extremely halophilic archaeon, Salarchaeum sp. strain JOR-1, was isolated from the east coast of the Dead Sea, Kingdom of Jordan, and sequenced using single-molecule real-time (SMRT) sequencing. The GC-rich 2.5-Mbp genome was composed of a circular chromosome and a megaplasmid. The genome contained 2,633 genes and was incorporated into HaloWeb (https://halo.umbc.edu/).

2021 ◽  
Vol 10 (28) ◽  
Author(s):  
Priya DasSarma ◽  
Brian P. Anton ◽  
Hedvig A. L. von Ehrenheim ◽  
Richard J. Roberts ◽  
Shiladitya DasSarma

An extremely halophilic archaeon, Halobacterium sp. strain GSL-19, was isolated from the north arm of Great Salt Lake in Utah. Single-molecule real-time (SMRT) sequencing was used to establish a GC-rich 2.3-Mbp genome composed of a circular chromosome and 2 plasmids, with 2,367 predicted genes, including 1 coding a CTAG-methylase widely distributed among Haloarchaea .


Author(s):  
Priya DasSarma ◽  
Brian P. Anton ◽  
Jessie M. Griffith ◽  
Karina S. Kunka ◽  
Richard J. Roberts ◽  
...  

Halobacterium sp. strain NRC-34001 is a red, extremely halophilic archaeon isolated in Canada in 1934. Single-molecule real-time sequencing revealed a 2.3-Mbp genome with a 2-Mbp chromosome and two plasmids (235 kb and 43 kb). The genome encodes all conserved core haloarchaeal groups (cHOGs) and a highly acidic proteome.


2021 ◽  
Vol 10 (34) ◽  
Author(s):  
Alexey Fomenkov ◽  
Priya DasSarma ◽  
Sean P. Kennedy ◽  
Richard J. Roberts ◽  
Shiladitya DasSarma

The genome of halophilic archaeon Haloarcula sinaiiensis ATCC 33800 was sequenced and assembled and comprises seven replicons. Four m6A and one m4C modified motifs and their responsible methyltransferase genes have been identified in the genome by single-molecule real-time (SMRT) sequencing and bioinformatic analysis.


2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Richard D. Morgan

Bacillus subtilis(Ehrenburg) Cohn ATCC 49760, deposited asBacillus globigii, is the source strain for the restriction enzymes BglI and BglII. Its complete sequence and full methylome were determined using single-molecule real-time (SMRT) sequencing.


2019 ◽  
Vol 8 (32) ◽  
Author(s):  
Phu Pwint Thin Hlaing ◽  
Ana Carolina M. Junqueira ◽  
Akira Uchida ◽  
Rikky W. Purbojati ◽  
James N. I. Houghton ◽  
...  

Brachybacterium sp. strain SGAir0954 was isolated from tropical air collected in Singapore, and its genome was sequenced and assembled using long reads generated by single-molecule real-time (SMRT) sequencing. The complete genome has a size of 3.41 Mb and consists of 2,955 protein coding genes, 50 tRNAs, and 9 rRNAs.


2021 ◽  
Vol 10 (48) ◽  
Author(s):  
Priya DasSarma ◽  
Brian P. Anton ◽  
Hedvig A. L. von Ehrenheim ◽  
Fabiana L. Martinez ◽  
Daniel Guzmán ◽  
...  

Halobacterium sp. strain BOL4-2 was isolated from an Andean salt flat, Salar de Uyuni, in Bolivia. Single-molecule real-time (SMRT) sequencing revealed a 2.4-Mbp genome with a 2.0-Mbp chromosome and four plasmids (2 to 299 kb). Its isolation from an environment experiencing multiple extremes makes the strain interesting for astrobiology.


2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Kazuma Nakano ◽  
Akino Shiroma ◽  
Hinako Tamotsu ◽  
Shun Ohki ◽  
Makiko Shimoji ◽  
...  

The first complete genome sequence of Lactobacillus curvatus was determined by PacBio RS II. The single circular chromosome (1,848,756 bp, G+C content of 42.1%) of L. curvatus FBA2, isolated from fermented vegetables, contained low G+C regions (26.9% minimum) and 43 sets of >1,000-bp identical sequence pairs. No plasmids were detected.


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