Optimization of Physical Parameters for the Enhanced Expression of Recombinant Chemokine Receptors D6 and DARC in Pichia pastoris

2021 ◽  
Vol 57 (S1) ◽  
pp. S78-S87
Author(s):  
W. Y. Tan ◽  
B. Y. Khoo ◽  
A. L. Chew
Keyword(s):  
Pichia Pastoris ◽  
Chemokine Receptors ◽  
Physical Parameters ◽  
Enhanced Expression

Enhanced Expression of Recombinant Elastase in Pichia pastoris through the Substitution of Thr for Ser in Asn-Xaa-Ser Sequons

2014 ◽  
Vol 175 (1) ◽  
pp. 428-435 ◽  
Author(s):  
Minghai Han ◽  
Weixian Wang ◽  
Xinfeng Wang ◽  
Xin Liu ◽  
Hui Cao ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Enhanced Expression

Co-expression of lipase isozymes for enhanced expression in Pichia pastoris

2017 ◽  
Vol 65 (4) ◽  
pp. 335-342 ◽  
Author(s):  
H. Cai ◽  
T. Zhang ◽  
M. Zhao ◽  
J. Mao ◽  
C. Cai ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Enhanced Expression

Enhanced expression of lipase I from Galactomyces geotrichum by codon optimisation in Pichia pastoris

2017 ◽  
Vol 138 ◽  
pp. 34-45 ◽  
Author(s):  
Hanzhen Qiao ◽  
Wenfei Zhang ◽  
Wutai Guan ◽  
Fang Chen ◽  
Shihai Zhang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Codon Optimisation ◽  
Enhanced Expression

Enhanced expression of recombinant elastase in Pichia pastoris through addition of N-glycosylation sites to the propeptide

2014 ◽  
Vol 36 (12) ◽  
pp. 2467-2471 ◽  
Author(s):  
Minghai Han ◽  
Weixian Wang ◽  
Gongcheng Jiang ◽  
Xinfeng Wang ◽  
Xin Liu ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Glycosylation Sites ◽  
Enhanced Expression

Integrated Strategies for Enhancing the Expression of Chitosanase AqCoA in the Pichia Pastoris by A Novel High-Copy Plasmid PMC-GAP

2021 ◽  
Author(s):  
Yanxin Wang ◽  
Yuqiang Zhao ◽  
Xue Luo ◽  
Zhoukun Li ◽  
Xianfeng Ye ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Protein Expression ◽  
Gene Dosage ◽  
Gene Copy Number ◽  
Maximum Activity ◽  
Gene Copy ◽  
Foreign Protein ◽  
Expression Levels ◽  
Model Combining ◽  
Enhanced Expression

Abstract In our previous study, the chitosanase AqCoA and its products chitooligosaccharides exhibited significant application in fungal disease protection. In this study, to enhance the expression of AqCoA, we obtained various strains with multi-copy by a novel plasmid pMC-GAP with stable transformation ability in Pichia pastoris and built an integrated model combining the gene copy number, the chaperones and protein production of AqCoA. In terms of gene dosage, the highest enzyme activity was 0.32 U/ml in the strain with four copies, which was 1.78-fold higher than that in strain with only one copy (0.18 U/ml). In addition, we found the chaperone like PDI, ERO1, HAC1, YDJ1, SSE1, SSA4 and SSO2 improved protein expression. Furthermore, the PDI/ERO1, SSA4/SSE1 and YDJ1/SSO2 pairs synergistically increased by 61%, 31% and 42% in expression levels of the strain GAP-1AqCoA. Finally, we investigated the effect co-expression of gene copy and chaperones on protein expression. The maximum activity reached 2.319 U/ml by the strain with six chaperones intergrant plus sixteen copies, which was 13-fold higher than that by the control strain with only one copy (GAP-1AqCoA). Co-expression of gene dosage and chaperones significantly enhanced expression levels of AqCoA, which presented a powerful tool to improve foreign protein expression.

Some Results of the Spectroscopic Study of Four Close Binary Systems of Early Spectral Types

1965 ◽  
Vol 5 ◽  
pp. 120-130
Author(s):  
T. S. Galkina
Keyword(s):  
Spectroscopic Study ◽  
Spectral Type ◽  
Spectroscopic Investigation ◽  
Binary Systems ◽  
Quantitative Information ◽  
Close Binary ◽  
Physical Parameters ◽  
Absorption And Emission ◽  
Close Binary Systems ◽  
Quantitative Estimates

It is necessary to have quantitative estimates of the intensity of lines (both absorption and emission) to obtain the physical parameters of the atmosphere of components.Some years ago at the Crimean observatory we began the spectroscopic investigation of close binary systems of the early spectral type with components WR, Of, O, B to try and obtain more quantitative information from the study of the spectra of the components.

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