Blood plasma protein adsorption capacity of perfluorocarbon emulsion stabilized by proxanol 268 (in vitro and in vivo Studies)

BIOPHYSICS ◽  
2012 ◽  
Vol 57 (2) ◽  
pp. 222-228 ◽  
Author(s):  
A. N. Sklifas ◽  
V. K. Zhalimov ◽  
A. A. Temnov ◽  
N. I. Kukushkin
Keyword(s):  
Blood Plasma ◽  
Protein Adsorption ◽  
Adsorption Capacity ◽  
Plasma Protein ◽  
In Vivo Studies ◽  
Perfluorocarbon Emulsion ◽  
Blood Plasma Protein ◽  
Plasma Protein Adsorption

scholarly journals A Bifunctional Adsorber Particle for the Removal of Hydrophobic Uremic Toxins from Whole Blood of Renal Failure Patients

Toxins ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 389 ◽  
Author(s):  
Marieke Sternkopf ◽  
Sven Thoröe-Boveleth ◽  
Tobias Beck ◽  
Kirsten Oleschko ◽  
Ansgar Erlenkötter ◽  
...  
Keyword(s):  
Adsorption Capacity ◽  
Binding Affinity ◽  
Whole Blood ◽  
Uremic Toxins ◽  
In Vivo Studies ◽  
Uremic Toxin ◽  
High Binding ◽  
High Binding Affinity

Hydrophobic uremic toxins accumulate in patients with chronic kidney disease, contributing to a highly increased cardiovascular risk. The clearance of these uremic toxins using current hemodialysis techniques is limited due to their hydrophobicity and their high binding affinity to plasma proteins. Adsorber techniques may be an appropriate alternative to increase hydrophobic uremic toxin removal. We developed an extracorporeal, whole-blood bifunctional adsorber particle consisting of a porous, activated charcoal core with a hydrophilic polyvinylpyrrolidone surface coating. The adsorption capacity was quantified using analytical chromatography after perfusion of the particles with an albumin solution or blood, each containing mixtures of hydrophobic uremic toxins. A time-dependent increase in hydrophobic uremic toxin adsorption was depicted and all toxins showed a high binding affinity to the adsorber particles. Further, the particle showed a sufficient hemocompatibility without significant effects on complement component 5a, thrombin-antithrombin III complex, or thrombocyte concentration in blood in vitro, although leukocyte counts were slightly reduced. In conclusion, the bifunctional adsorber particle with cross-linked polyvinylpyrrolidone coating showed a high adsorption capacity without adverse effects on hemocompatibility in vitro. Thus, it may be an interesting candidate for further in vivo studies with the aim to increase the efficiency of conventional dialysis techniques.

Surface properties of nanocrystalline TiO 2 coatings in relation to the in vitro plasma protein adsorption

2015 ◽  
Vol 10 (4) ◽  
pp. 045012 ◽  
Author(s):  
M Lorenzetti ◽  
G Bernardini ◽  
T Luxbacher ◽  
A Santucci ◽  
S Kobe ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Surface Properties ◽  
Plasma Protein ◽  
Plasma Protein Adsorption

Plasma protein adsorption: In vitro and ex vivo observations

1988 ◽  
Vol 17 (1) ◽  
pp. 453-465 ◽  
Author(s):  
W. G. Pitt ◽  
B. R. Young ◽  
K. Park ◽  
S. L. Cooper
Keyword(s):  
Protein Adsorption ◽  
Plasma Protein ◽  
Ex Vivo ◽  
Plasma Protein Adsorption

scholarly journals P1101A BIFUNCTIONAL ADSORBER PARTICLE FOR THE REMOVAL OF HYDROPHOBIC UREMIC TOXINS FROM WHOLE BLOOD OF RENAL FAILURE PATIENTS

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Marieke Sternkopf ◽  
Thimoteus Speer ◽  
Claudia Göttsch ◽  
Vera Jankowski ◽  
Joachim Jankowski ◽  
...  
Keyword(s):  
Adsorption Capacity ◽  
Binding Affinity ◽  
Whole Blood ◽  
Uremic Toxins ◽  
In Vivo Studies ◽  
Uremic Toxin ◽  
High Binding ◽  
High Binding Affinity

Abstract Background and Aims Hydrophobic uremic toxins accumulate in patients with chronic kidney disease, contributing to a highly increased cardiovascular risk. The clearance of these uremic toxins using current hemodialysis techniques is limited due to their hydrophobicity and their high binding affinity to plasma proteins. Adsorber techniques may be an appropriate alternative to increase hydrophobic uremic toxin removal. Method We developed an extracorporeal, whole-blood bifunctional adsorber particle consisting of a porous, activated charcoal core with a hydrophilic polyvinylpyrrolidone surface coating. The adsorption capacity was quantified using analytical chromatography after perfusion of the particles with an albumin solution or blood, each containing mixtures of hydrophobic uremic toxins. Results A time-dependent increase in hydrophobic uremic toxin adsorption was depicted and all toxins showed a high binding affinity to the adsorber particles. Further, the particle showed a sufficient hemocompatibility without significant effects on complement component 5a, thrombin-antithrombin III complex, or thrombocyte concentration in blood in vitro, although leukocyte counts were slightly reduced. Conclusion In conclusion, the bifunctional adsorber particle with cross-linked polyvinylpyrrolidone coating showed a high adsorption capacity without adverse effects on hemocompatibility in vitro. Thus, it may be an interesting candidate for further in vivo studies with the aim to increase the efficiency of conventional dialysis techniques.

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