Activity of promoters of carnation etched ring virus and dahlia mosaic virus in tobacco protoplasts and transgenic plants

2008 ◽  
Vol 55 (5) ◽  
pp. 687-693 ◽  
Author(s):  
B. R. Kuluev ◽  
A. V. Knyazev ◽  
A. V. Chemeris
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Tobacco Protoplasts ◽  
Dahlia Mosaic Virus

Analysis of Dahlia Mosaic Virus Full-length Transcript Promoter-Driven Gene Expression in Transgenic Plants

2014 ◽  
Vol 33 (2) ◽  
pp. 178-199 ◽  
Author(s):  
Dipak Kumar Sahoo ◽  
Shayan Sarkar ◽  
Sumita Raha ◽  
Narayan Chandra Das ◽  
Joydeep Banerjee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Mosaic Virus ◽  
Full Length ◽  
Dahlia Mosaic Virus ◽  
Full Length Transcript

scholarly journals Altered Patterns of Gene Expression in Arabidopsis Elicited by Cauliflower Mosaic Virus (CaMV) Infection and by a CaMV Gene VI Transgene

1999 ◽  
Vol 12 (5) ◽  
pp. 377-384 ◽  
Author(s):  
Chiara Geri ◽  
Edi Cecchini ◽  
Maria E. Giannakou ◽  
Simon N. Covey ◽  
Joel J. Milner
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Mosaic Virus ◽  
Rna Binding ◽  
Important Determinant ◽  
Blot Hybridization ◽  
Cauliflower Mosaic Virus ◽  
Slot Blot Hybridization ◽  
Pcr Products ◽  
Polymerase Chain

Cauliflower mosaic virus (CaMV) gene VI protein (P6) is an important determinant of symptom expression. Differential display polymerase chain reaction (PCR) was used to identify changes in gene expression in Arabidopsis elicited by a P6 transgene that causes a symptomatic phenotype. We used slot blot hybridization to measure the abundance of mRNAs complementary to 66 candidate PCR products in transgenic, CaMV-infected, and uninfected Arabidopsis plants. CaMV-infected and P6 transgenic plants showed broadly similar changes in abundance of mRNA species. In P6 transgenic plants we detected 18 PCR products that showed unambiguous changes in abundance plus another 15 that showed more limited changes (approximately twofold). CaMV-infected plants showed 17 unambiguous and 13 limited changes. Down-regulated species include those encoding a novel, phenol-like sulfotransferase, and a glycine-rich, RNA-binding protein. Up-regulated species included ones encoding an myb protein, glycine-rich and stress-inducible proteins, and a member of a previously unreported gene family. CaMV infection causes alterations in expression of many Arabidopsis genes. Transgene-mediated expression of P6 mimics virus infection in its effect on host gene expression, providing a potential mechanism for this process.

scholarly journals Involvement of the chloroplast gene ferredoxin 1 in multiple responses of Nicotiana benthamiana to Potato virus X infection

2019 ◽  
Vol 71 (6) ◽  
pp. 2142-2156 ◽  
Author(s):  
Xue Yang ◽  
Yuwen Lu ◽  
Fang Wang ◽  
Ying Chen ◽  
Yanzhen Tian ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Potato Virus ◽  
Tobacco Rattle Virus ◽  
Potato Virus X ◽  
Tomato Mosaic Virus ◽  
Pcr Analysis ◽  
Coat Proteins ◽  
Virus Induced Gene Silencing ◽  
Callose Deposition

Abstract The chloroplast protein ferredoxin 1 (FD1), with roles in the chloroplast electron transport chain, is known to interact with the coat proteins (CPs) of Tomato mosaic virus and Cucumber mosaic virus. However, our understanding of the roles of FD1 in virus infection remains limited. Here, we report that the Potato virus X (PVX) p25 protein interacts with FD1, whose mRNA and protein levels are reduced by PVX infection or by transient expression of p25. Silencing of FD1 by Tobacco rattle virus-based virus-induced gene silencing (VIGS) promoted the local and systemic infection of plants by PVX. Use of a drop-and-see (DANS) assay and callose staining revealed that the permeability of plasmodesmata (PDs) was increased in FD1-silenced plants together with a consistently reduced level of PD callose deposition. After FD1 silencing, quantitative reverse transcription–real-time PCR (qRT–PCR) analysis and LC-MS revealed these plants to have a low accumulation of the phytohormones abscisic acid (ABA) and salicylic acid (SA), which contributed to the decreased callose deposition at PDs. Overexpression of FD1 in transgenic plants manifested resistance to PVX infection, but the contents of ABA and SA, and the PD callose deposition were not increased in transgenic plants. Overexpression of FD1 interfered with the RNA silencing suppressor function of p25. These results demonstrate that interfering with FD1 function causes abnormal plant hormone-mediated antiviral processes and thus enhances PVX infection.

scholarly journals Alfalfa mosaic virus RNA3 mutants do not replicate in transgenic plants expressing RNA3-specific genes

1991 ◽  
Vol 72 (2) ◽  
pp. 253-258 ◽  
Author(s):  
J.-M. Dore ◽  
C. M. P. van Dun ◽  
L. Pinck ◽  
J. F. Bol
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Alfalfa Mosaic Virus

scholarly journals Production of Human Papillomavirus Type 16 Virus-Like Particles in Transgenic Plants

2003 ◽  
Vol 77 (17) ◽  
pp. 9211-9220 ◽  
Author(s):  
Sophia Biemelt ◽  
Uwe Sonnewald ◽  
Petra Galmbacher ◽  
Lothar Willmitzer ◽  
Martin Müller
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Protein Modification ◽  
Human Papillomaviruses ◽  
Cauliflower Mosaic Virus ◽  
Total Soluble Protein ◽  
Virus Like Particles ◽  
Potato Plants ◽  
L1 Protein

ABSTRACT Cervical cancer is linked to infection with human papillomaviruses (HPV) and is the third most common cancer among women worldwide. There is a strong demand for the development of an HPV preventive vaccine. Transgenic plants expressing the HPV major capsid protein L1 could be a system to produce virus-like particles for prophylactic vaccination or could even be used as edible vaccines to induce an L1-specific prophylactic immune response. Here, we describe the generation of transgenic tobacco and potato plants carrying the HPV type 16 major structural gene L1 under the control of the cauliflower mosaic virus 35S promoter. All attempts to express either the original, unmodified L1 gene or an L1 gene with a codon usage optimized for expression in plants failed. Surprisingly, small amounts of the protein were detected using an L1 gene optimized for expression in human cells. However, Northern blot analysis revealed that most of the L1 transcripts were degraded. Introduction of the translational enhancer Ω derived from the tobacco mosaic virus strongly increased transcript stability and resulted in accumulation of L1 protein to approximately 0.5 to 0.2% of total soluble protein in transgenic tobacco and potato plants, respectively. The plant-derived L1 protein displayed conformation-specific epitopes and assembled into virus-like particles. Furthermore, we did not find any indications of protein modification of the L1 protein produced in plants. Plant-derived L1 was as immunogenic as L1 expressed in baculovirus-infected insect cells. Feeding of tubers from transgenic potatoes to mice induced an anti-L1 antibody response in 3 out of 24 mice, although this response was only transient in two of the mice. Our data, however, indicate that an anti-L1 response was primed in about half of the 24 animals.

scholarly journals Deep Sequencing of Recombinant Virus Populations in Transgenic and Nontransgenic Plants Infected with Cucumber mosaic virus

2013 ◽  
Vol 26 (7) ◽  
pp. 801-811 ◽  
Author(s):  
Marco Morroni ◽  
Mireille Jacquemond ◽  
Mark Tepfer
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
De Novo ◽  
Recombinant Viruses ◽  
Viral Genomes ◽  
Large Deletions ◽  
Cp Gene ◽  
Viral Sequences ◽  
Virus Variability

Recombination is a major source of virus variability, and the question of whether novel recombinant viruses would emerge in transgenic plants expressing viral sequences has been a biosafety issue. We describe the results of pyrosequencing the recombinant viral RNAs appearing in transgenic plants expressing the coat protein (CP) gene and 3′ noncoding region of Cucumber mosaic virus RNA3, as well as in nontransgenic controls. The populations of recombinants in both transgenic and nontransgenic plants were similar to those previously described from Sanger sequencing but many more recombinant types were observed, including a novel class of large deletions removing all or nearly the entire CP gene. These results show that populations of recombinant viral genomes arising de novo can be characterized in detail by pyrosequencing, and confirm that the transgenic plants did not harbor novel recombinants of biosafety concern.

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