The distribution of mitoses in the imaginal disks of third-instar Drosophila melanogaster larvae

2007 ◽  
Vol 43 (7) ◽  
pp. 769-775 ◽  
Author(s):  
L. V. Omel’yanchuk ◽  
C. Nokkala ◽  
J. Mattila ◽  
L. I. Lebedeva ◽  
T. Yu. Baimak ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Imaginal Disks

Tissue-specific variation in Hsp70 expression and thermal damage in Drosophila melanogaster larvae.

1997 ◽  
Vol 200 (14) ◽  
pp. 2007-2015 ◽  
Author(s):  
R A Krebs ◽  
M E Feder
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Trypan Blue ◽  
Fat Body ◽  
Malpighian Tubules ◽  
Blue Staining ◽  
Hsp70 Expression ◽  
Shock Temperature ◽  
Specific Variation ◽  
Imaginal Disks

All tissues of larval Drosophila melanogaster express Hsp70, the major heat-shock protein of this species, after both mild (36 degrees C) and severe (38.5 degrees C) heat shock. We used Hsp70-specific immunofluorescence to compare the rate and intensity of Hsp70 expression in various tissues after these two heat-shock treatments, and to compare this with related differences in the intensity of Trypan Blue staining shown by the tissues. Trypan Blue is a marker of tissue damage. Hsp70 was rarely detectable before heat shock. Brain, salivary glands, imaginal disks and hindgut expressed Hsp70 within the first hour of heat shock, whereas gut tissues, fat body and Malpighian tubules did not express Hsp70 until 4-21 h after heat shock. Differences in Hsp70 expression between tissues were more pronounced at the higher heat-shock temperature. Tissues that expressed Hsp70 slowly stained most intensely with Trypan Blue. Gut stained especially intensely, which suggests that its sensitivity to heat shock may limit larval thermotolerance. These patterns further suggest that some cells respond primarily to damage caused by heat shock rather than to elevated temperature per se and/or that Hsp70 expression is itself damaged by heat and requires time for recovery in some tissues.

Regulative interactions between cells from different imaginal disks of Drosophila melanogaster

Science ◽  
1978 ◽  
Vol 201 (4359) ◽  
pp. 928-930 ◽  
Author(s):  
P. Bryant ◽  
P. Adler ◽  
C Duranceau ◽  
M. Fain ◽  
S Glenn ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Imaginal Disks

scholarly journals Transgenic Analysis of the Smad Family of TGF-β Signal Transducers in Drosophila melanogaster Suggests New Roles and New Interactions Between Family Members

Genetics ◽  
2001 ◽  
Vol 157 (4) ◽  
pp. 1639-1648 ◽  
Author(s):  
Raymond M Marquez ◽  
Matthew A Singer ◽  
Norma T Takaesu ◽  
W Ross Waldrip ◽  
Yevgenya Kraytsberg ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Transforming Growth Factor ◽  
Family Members ◽  
Transforming Growth Factor Β ◽  
Signal Transducers ◽  
New Information ◽  
Imaginal Disks ◽  
New Interactions ◽  
Potential Interactions ◽  
Individual Human

Abstract Smad signal transducers are required for transforming growth factor-β-mediated developmental events in many organisms including humans. However, the roles of individual human Smad genes (hSmads) in development are largely unknown. Our hypothesis is that an hSmad performs developmental roles analogous to those of the most similar Drosophila Smad gene (dSmad). We expressed six hSmad and four dSmad transgenes in Drosophila melanogaster using the Gal4/UAS system and compared their phenotypes. Phylogenetically related human and Drosophila Smads induced similar phenotypes supporting the hypothesis. In contrast, two nearly identical hSmads generated distinct phenotypes. When expressed in wing imaginal disks, hSmad2 induced oversize wings while hSmad3 induced cell death. This observation suggests that a very small number of amino acid differences, between Smads in the same species, confer distinct developmental roles. Our observations also suggest new roles for the dSmads, Med and Dad, in dActivin signaling and potential interactions between these family members. Overall, the study demonstrates that transgenic methods in Drosophila can provide new information about non-Drosophila members of developmentally important multigene families.

scholarly journals Tissue localization of Drosophila melanogaster ras transcripts during development.

1986 ◽  
Vol 6 (6) ◽  
pp. 2241-2248 ◽  
Author(s):  
D Segal ◽  
B Z Shilo
Keyword(s):  
Drosophila Melanogaster ◽  
Tissue Distribution ◽  
Adult Stage ◽  
Multiple Roles ◽  
Similar Distribution ◽  
Ras Proteins ◽  
Dividing Cells ◽  
Imaginal Disks ◽  
The Brain

Three ras homologs have been identified in Drosophila melanogaster. Here we describe the tissue distribution of their transcripts as analyzed by in situ hybridization. The RNAs of the three genes show a similar distribution at every developmental stage examined. In embryos, the transcripts are uniformly distributed. In larvae, ras transcripts are restricted to dividing cells (e.g., imaginal disks, gonads, and brain). At the adult stage, several tissues contain ras transcripts. The strongest hybridization signals are localized to the adult ovaries and to the cortex of the brain and ganglia, which at this stage are comprised of differentiated, nondividing cells. The tissue distribution of ras transcripts in D. melanogaster suggests that the ras proteins have multiple roles during development which may be related to both the proliferative and differentiated states of the tissues.

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