By using a new kind of single-beam normalized photo-acoustic tomography spectroscopy (PAS-CT) technology with non-damage detection, the photo-acoustic tomography spectroscopy, optical absorption properties and photosynthetic pigment content of green and red leaves ofBischofia javanicawere studied. The results show that: the photo-acoustic tomography spectroscopy ofBischofia javanicaleaves could be obtained from different chopping frequency and different sample positions, and photosynthetic pigment content of their leaves is closely related to their photosynthetic intensity. The more photosynthetic pigment content the leaves ofBischofia javanicacontain, the bigger optical absorption coefficient and the higher photosynthetic efficiency they have. The photosynthetic pigment content of the green leaves ofBischofia javanicais higher than that of the red ones. As a result, the photosynthesis of green leaves is better than that of the red ones. According to the results of our research, the photosynthetic efficiency ofBischofia javanicacould be improved and its growth time could be effectively controlled to enhance the growth ofBischofia javanica. Furthermore, it could play an important role on the development of forestry, and meet the need of forest for 6 billion people. The research showed high science value to study and applications of the photosynthesis of plants.