Determination of phenobarbital in human urine and serum using flow injection chemiluminescence

2011 ◽  
Vol 5 (2) ◽  
pp. 184-187 ◽  
Author(s):  
X. Li ◽  
L. C. Niu ◽  
X. L. He ◽  
Z. H. Song
Keyword(s):  
Flow Injection ◽  
Human Urine ◽  
Human Urine And Serum ◽  
Flow Injection Chemiluminescence ◽  
Urine And Serum

scholarly journals Subnanogram Determination of Aniracetam in Pharmaceutical Preparations and Biofluids by Flow Injection Analysis with Chemiluminescence Detection Based on Its Enhancement of the Myoglobin-Luminol Reaction

2011 ◽  
Vol 94 (5) ◽  
pp. 1461-1466
Author(s):  
Shao Xiaodong ◽  
Li Ying ◽  
Li Fagen ◽  
Liu Yangqin ◽  
Song Zhenghua
Keyword(s):  
Flow Injection ◽  
Pharmaceutical Preparations ◽  
Serum Samples ◽  
Reaction Conditions ◽  
Whole Process ◽  
Optimum Reaction ◽  
Human Urine And Serum ◽  
Flow Injection Chemiluminescence ◽  
Urine And Serum

Abstract A novel flow injection chemiluminescence method with a myoglobin-luminol system is described for determining aniracetam. Myoglobin-bound aniracetam produced a complex that catalyzed the chemiluminescence reaction between luminol and myoglobin, leading to fast chemiluminescence. The chemiluminescence intensity in the presence of aniracetam was remarkably enhanced compared with that in the absence of aniracetam. Under the optimum reaction conditions the chemiluminescence increment produced was proportional to the concentration of aniracetam in the range of 0.1–1000.0 ng/mL (R2 = 0.9992), with a detection limit of 0.03 ng/mL (3δ). At a flow rate of 2.0 mL/min, the whole process, including sampling and washing, could be completed in 0.5 min, offering a sampling efficiency of 120/h; the RSD was less than 3.0% (n = 5). The method was satisfactory for determination of aniracetam in pharmaceutical preparations and human urine and serum samples. A possible mechanism of the reaction is also discussed.

Micellar‐Enhanced Spectrofluorimetric Determination of Trazodone Hydrochloride in Human Urine and Serum

2007 ◽  
Vol 40 (1) ◽  
pp. 151-162 ◽  
Author(s):  
Gong‐Jun Yang ◽  
Ping Liu ◽  
Xi‐Long Qu ◽  
Ming‐Shen ◽  
Chen‐Ying Wang ◽  
...  
Keyword(s):  
Human Urine ◽  
Spectrofluorimetric Determination ◽  
Human Urine And Serum ◽  
Urine And Serum ◽  
Trazodone Hydrochloride

scholarly journals Determination of phenobarbital in human urine and serum usingflow injection chemiluminescence

2012 ◽  
Vol 58 (1) ◽  
pp. 88-94
Author(s):  
K. Li ◽  
L.Z. Nu ◽  
K.L. Khe ◽  
K.H. Song
Keyword(s):  
Human Urine ◽  
Pharmaceutical Preparations ◽  
Injection System ◽  
Chemiluminescence Method ◽  
Chemiluminescence Intensity ◽  
Complete Determination ◽  
Flow Injection System ◽  
Human Urine And Serum ◽  
Urine And Serum

A sensitive chemiluminescence method, based on the enhancive effect of phenobarbital on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of phenobarbital. The chemiluminescence intensity responded to the concentration of phenobarbital linearly ranging from 0.05 to 10 ng⋅ml-1 with the detection limit of 0.02 ng⋅ml-1 (3σ). At a flow rate of 2.0 ml⋅min-1, a complete determination of phenobarbital, including sampling and washing, could be accomplished in 0.5 min, offering the sampling efficiency of 120 h-1 accordingly. The method was applied successfully in an assay of PB for pharmaceutical preparations, human urine and serum without any pretreatment with recovery from 95.7 to 106.7% and RSDs of less than 3.0%.

Fluorimetric determination of isatin in human urine and serum by liquid chromatography postcolumn photoirradiation

The Analyst ◽  
2001 ◽  
Vol 126 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Ken-ichi Mawatari ◽  
Makiko Segawa ◽  
Rumiko Masatsuka ◽  
Yasuko Hanawa ◽  
Fumio Iinuma ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Human Urine ◽  
Fluorimetric Determination ◽  
Human Urine And Serum ◽  
Urine And Serum

Determination of beta 2-microglobulin in human urine and serum by latex immunoassay.

1981 ◽  
Vol 27 (6) ◽  
pp. 832-837 ◽  
Author(s):  
A M Bernard ◽  
A Vyskocil ◽  
R R Lauwerys
Keyword(s):  
Human Urine ◽  
Albumin Solution ◽  
Coated Particles ◽  
Analytical Recovery ◽  
Highly Sensitive ◽  
Human Urine And Serum ◽  
Beta 2 ◽  
Beta 2 Microglobulin ◽  
Urine And Serum

Abstract This highly sensitive method for determination of beta 2-microglobulin (beta 2-m) in human urine or serum is based on direct agglutination by beta 2-m of latex particles on which an antibody against beta 2-m is adsorbed. The agglutination is quantified by counting the remaining unagglutinated particles, or by turbidimetry. A novel aspect of this method is the capability to prevent nonspecific agglutination of the antibody-coated particles by diluting them with an albumin solution of well-defined characteristics (pH, freshness, concentration) just before the assay. The assayable concentration range is 1--32 micrograms/L, the detection limit 0.5 micrograms/L. Within-assay CV, based on 10 determinations of beta 2-m in urine and serum at two different dilutions, ranged from 4.6 to 8.7%. Between-assay CV, calculated from 10 determinations of beta 2-m in urine and serum, was 10 and 8.4%, respectively. Analytical recovery of beta 2-m in urine averaged 97% and in serum 104% (n = 10). No component of urine or serum interfered. Coefficients of correlation for beta 2-m in urine or serum as measured by radioimmunoassay and latex immunoassay were 0.97 and 0.93, respectively. Concentrations of beta 2-m in serum and urine from 33 healthy men (ages 20 to 67 years) averaged 1.5 mg/l and 54 micrograms/g of creatine, respectively.

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