Abstract
A fully automated microfluidic Flow Injection spectrophotometric system was designed in our laboratory with methylene blue used as an example. The device consists of a double-line microfluidic chip with dimensions (30 μl x 4 cm), each 15 μl volume intended to allow a very small volume of reagent and sample to be used. The microfluidic system also consists of two types of Arduino software. The first was the UNO of a type used to control the two mini peristaltic pumps to drive the water and methylene blue as carrier stream and sample respectively. The Mega type, which was equipped with homemade software, signal-to-peak, was the second Arduino which used as a data logger to record the results in the form of peaks using Microsoft Excel 2016. The peak’s height was corresponding to concentration. The linearity was in the range (0.025-0.125 μg/ml) with a five-point regression coefficient (0.9981), RSD% for ten replicates is 0.025 μg/mL. The detection limit was (0.001 μg/ml) and the sample throughput was 300 samples per hour and each sample required 25 μl of chemical reagents. Therefore, 240 samples need only (5.0 ml) from the chemical reagents, which clearly indicates that the consumed reagent and the waste were very low. Therefore, the method for determining methylene blue with a fully automated system is very environmentally friendly. save time, consumption regent, Improve quality, decrease cost-consumption reducing the consume of sample and inherited the reproducibility and accurate from flow injection analysis.
Lab build Fully Automated microfluduic flow injection System
