New Polyethylenimine-Based Polycationic Polymers with Plant Additives for Immobilizing Phototrophic Microorganisms

2020 ◽  
Vol 15 (1) ◽  
pp. 28-36
Author(s):  
S. G. Vasilieva ◽  
E. S. Lobakova ◽  
A. S. Morozov ◽  
K. A. Shibzuhova ◽  
M. V. Titova ◽  
...  
Keyword(s):  
Phototrophic Microorganisms ◽  
Polycationic Polymers

scholarly journals Cyanobakterien als Biokatalysatoren

BIOspektrum ◽  
2021 ◽  
Vol 27 (2) ◽  
pp. 208-210
Author(s):  
Marc M. Nowaczyk ◽  
Hanna C. Grimm ◽  
Leen Assil-Companioni ◽  
Robert Kourist
Keyword(s):  
Natural Process ◽  
Oxygenic Photosynthesis ◽  
Phototrophic Microorganisms ◽  
Electron Sink ◽  
Pcc 6803

AbstractThe highly optimized natural process of oxygenic photosynthesis leads to the formation of redox equivalents, such as NADPH, that can be used to fuel heterologous biotransformations in phototrophic microorganisms. We investigated the reduction of 2-methylmaleimide by the ene-reductase YqjM in the cyanobacterium Synechocystis sp. PCC 6803 and doubled the productivity of the cells by inactivating flavodiironproteins (FDPs) as competing electron sink under self-shading conditions, reaching 18.3 mmol h−1 L−1.

scholarly journals Novel Cell Permeable Polymers of N-Substituted L-2,3-Diaminopropionic Acid (DAPEGs) and Cellular Consequences of Their Interactions with Nucleic Acids

2021 ◽  
Vol 22 (5) ◽  
pp. 2571
Author(s):  
Anita Romanowska ◽  
Katarzyna Węgrzyn ◽  
Katarzyna Bury ◽  
Emilia Sikorska ◽  
Aleksandra Gnatek ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Plasmid Dna ◽  
Main Chain ◽  
Cell Permeability ◽  
Binding Ability ◽  
Transfection Efficacy ◽  
Polycationic Polymers ◽  
Diaminopropionic Acid ◽  
Dsdna Binding ◽  
Micromolar Range

The present study aimed to synthesize novel polycationic polymers composed of N-substituted L-2,3-diaminopropionic acid residues (DAPEGs) and investigate their cell permeability, cytotoxicity, and DNA-binding ability. The most efficient cell membrane-penetrating compounds (O2Oc-Dap(GO2)n-O2Oc-NH2, where n = 4, 6, and 8) showed dsDNA binding with a binding constant in the micromolar range (0.3, 3.4, and 0.19 µM, respectively) and were not cytotoxic to HB2 and MDA-MB-231 cells. Selected compounds used in the transfection of a GFP plasmid showed high transfection efficacy and minimal cytotoxicity. Their interaction with plasmid DNA and the increasing length of the main chain of tested compounds strongly influenced the organization and shape of the flower-like nanostructures formed, which were unique for 5/6-FAM-O2Oc-[Dap(GO2)]8-O2Oc-NH2 and typical for large proteins.

scholarly journals The influence of inherent properties of building limestones on their bioreceptivity to phototrophic microorganisms

2009 ◽  
Vol 59 (4) ◽  
pp. 705-713 ◽  
Author(s):  
Ana Zélia Miller ◽  
Amélia Dionísio ◽  
Leonila Laiz ◽  
Maria Filomena Macedo ◽  
Cesareo Saiz-Jimenez
Keyword(s):  
Phototrophic Microorganisms

Interaction of Polycationic Polymers with Supported Lipid Bilayers and Cells:  Nanoscale Hole Formation and Enhanced Membrane Permeability

2006 ◽  
Vol 17 (3) ◽  
pp. 728-734 ◽  
Author(s):  
Seungpyo Hong ◽  
Pascale R. Leroueil ◽  
Elizabeth K. Janus ◽  
Jennifer L. Peters ◽  
Mary-Margaret Kober ◽  
...  
Keyword(s):  
Membrane Permeability ◽  
Lipid Bilayers ◽  
Supported Lipid Bilayers ◽  
Hole Formation ◽  
Polycationic Polymers

scholarly journals The CRG-PVA hydrogels study of properties with various nanoparticles and their application for cultivation of phototrophic microorganisms

2018 ◽  
Vol 292 ◽  
pp. 012051 ◽  
Author(s):  
K V Gorin ◽  
G U Badranova ◽  
P M Gotovtsev ◽  
A Yu Shatalova ◽  
T E Grigoriev ◽  
...  
Keyword(s):  
Phototrophic Microorganisms

scholarly journals Influence of Alkyl Chains of Modified Polysuccinimide‐Based Polycationic Polymers on Polyplex Formation and Transfection

2019 ◽  
Vol 19 (10) ◽  
pp. 1900117 ◽  
Author(s):  
Marcelo H. Kravicz ◽  
Debora T. Balogh ◽  
Mrityunjoy Kar ◽  
Stefanie Wedepohl ◽  
Maria Vitoria L. B. Bentley ◽  
...  
Keyword(s):  
Alkyl Chains ◽  
Polycationic Polymers
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