A6.08 Transcriptome profiling by next generation sequencing of hematopoietic progenitors in murine systemic lupus erythematosus (SLE)

2016 ◽  
Vol 75 (Suppl 1) ◽  
pp. A50.1-A50
Author(s):  
A Banos ◽  
M Grigoriou ◽  
P Verginis ◽  
P Pavlidis ◽  
G Bertsias ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Next Generation Sequencing ◽  
Lupus Erythematosus ◽  
Transcriptome Profiling ◽  
Next Generation ◽  
Hematopoietic Progenitors ◽  
Systemic Lupus ◽  
Murine Systemic Lupus Erythematosus ◽  
Generation Sequencing

scholarly journals Rapid and precise diagnosis of pneumonia coinfected by Pneumocystis jirovecii and Aspergillus fumigatus assisted by next-generation sequencing in a patient with systemic lupus erythematosus: a case report

2021 ◽  
Author(s):  
Yili Chen ◽  
Lu Ai ◽  
Yingqun Zhou ◽  
Yating Zhao ◽  
Jianyu Huang ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Next Generation Sequencing ◽  
Aspergillus Fumigatus ◽  
Lupus Erythematosus ◽  
Financial Burden ◽  
Pneumocystis Jirovecii ◽  
Clinical Approach ◽  
Next Generation ◽  
Systemic Lupus ◽  
Generation Sequencing

Abstract Background: Pneumocystis jirovecii and Aspergillus fumigatus, are opportunistic pathogenic fungus that has a major impact on mortality in patients with systemic lupus erythematosus. With the potential to invade multiple organs, early and accurate diagnosis is essential to the survival of SLE patients, establishing an early diagnosis of the infection, especially coinfection by Pneumocystis jirovecii and Aspergillus fumigatus, still remains a great challenge.Case presentation: In this case, we reported that the application of next-generation sequencing in diagnosing Pneumocystis jirovecii and Aspergillus fumigatus coinfection in a Chinese girl with systemic lupus erythematosus (SLE). Voriconazole was used to treat pulmonary aspergillosis, besides sulfamethoxazole and trimethoprim (SMZ-TMP), and caspofungin acetate to treat Pneumocystis jirovecii infection for 6 days. On Day 10 of admission, her chest radiograph displayed obvious absorption of bilateral lung inflammation though the circumstance of repeated fever had not improved. Unfortunately, the patient discharged from the hospital since the financial burden, therefore further condition couldn't be tracked.Conclusions: This successful application of the next generation sequencing assisting the rapid diagnosis of Pneumocystis jirovecii and Aspergillus fumigatus coinfection provides a new perspective in the clinical approach against the systematic fungi infections and highlights the potential of this technique in rapid etiological diagnosis.

scholarly journals Rapid and Precise Diagnosis of Pneumonia Coinfected by Pneumocystis Jirovecii and Aspergillus Fumigatus Assisted by Next-generation Sequencing in a Patient With Systemic Lupus Erythematosus: a Case Report

2021 ◽  
Author(s):  
Yili Chen ◽  
Lu Ai ◽  
Yingqun Zhou ◽  
Yating Zhao ◽  
Jianyu Huang ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Next Generation Sequencing ◽  
Aspergillus Fumigatus ◽  
Lupus Erythematosus ◽  
Financial Burden ◽  
Pneumocystis Jirovecii ◽  
Clinical Approach ◽  
Next Generation ◽  
Systemic Lupus ◽  
Generation Sequencing

Abstract Background: Pneumocystis jirovecii and Aspergillus fumigatus, are opportunistic pathogenic fungus that has a major impact on mortality in patients with systemic lupus erythematosus. With the potential to invade multiple organs, early and accurate diagnosis is essential to the survival of SLE patients, establishing an early diagnosis of the infection, especially coinfection by Pneumocystis jirovecii and Aspergillus fumigatus, still remains a great challenge.Case presentation: In this case, we reported that the application of next-generation sequencing in diagnosing Pneumocystis jirovecii and Aspergillus fumigatus coinfection in a Chinese girl with systemic lupus erythematosus (SLE). Voriconazole was used to treat pulmonary aspergillosis, besides sulfamethoxazole and trimethoprim (SMZ-TMP), and caspofungin acetate to treat Pneumocystis jirovecii infection for 6 days. On Day 10 of admission, her chest radiograph displayed obvious absorption of bilateral lung inflammation though the circumstance of repeated fever had not improved. Unfortunately, the patient discharged from the hospital since the financial burden, therefore further condition couldn't be tracked.Conclusions: This successful application of the next generation sequencing assisting the rapid diagnosis of Pneumocystis jirovecii and Aspergillus fumigatus coinfection provides a new perspective in the clinical approach against the systematic fungi infections and highlights the potential of this technique in rapid etiological diagnosis.

scholarly journals Rapid and precise diagnosis of pneumonia coinfected by Pneumocystis jirovecii and Aspergillus fumigatus assisted by next-generation sequencing in a patient with systemic lupus erythematosus: a case report

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Yili Chen ◽  
Lu Ai ◽  
Yingqun Zhou ◽  
Yating Zhao ◽  
Jianyu Huang ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Next Generation Sequencing ◽  
Aspergillus Fumigatus ◽  
Lupus Erythematosus ◽  
Financial Burden ◽  
Pneumocystis Jirovecii ◽  
Clinical Approach ◽  
Next Generation ◽  
Systemic Lupus ◽  
Generation Sequencing

Abstract Background Pneumocystis jirovecii and Aspergillus fumigatus, are opportunistic pathogenic fungus that has a major impact on mortality in patients with systemic lupus erythematosus. With the potential to invade multiple organs, early and accurate diagnosis is essential to the survival of SLE patients, establishing an early diagnosis of the infection, especially coinfection by Pneumocystis jirovecii and Aspergillus fumigatus, still remains a great challenge. Case presentation In this case, we reported that the application of next -generation sequencing in diagnosing Pneumocystis jirovecii and Aspergillus fumigatus coinfection in a Chinese girl with systemic lupus erythematosus (SLE). Voriconazole was used to treat pulmonary aspergillosis, besides sulfamethoxazole and trimethoprim (SMZ-TMP), and caspofungin acetate to treat Pneumocystis jirovecii infection for 6 days. On Day 10 of admission, her chest radiograph displayed obvious absorption of bilateral lung inflammation though the circumstance of repeated fever had not improved. Unfortunately, the patient discharged from the hospital since the financial burden, and during the follow-up, it was documented the patient died within one week after discharge. Conclusions This successful application of the next generation sequencing assisting the rapid diagnosis of Pneumocystis jirovecii and Aspergillus fumigatus coinfection provides a new perspective in the clinical approach against the systematic fungi infections and highlights the potential of this technique in rapid etiological diagnosis.

scholarly journals Erratum: Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study

2014 ◽  
Vol 32 (11) ◽  
pp. 1166-1166 ◽  
Author(s):  
Sheng Li ◽  
Scott W Tighe ◽  
Charles M Nicolet ◽  
Deborah Grove ◽  
Shawn Levy ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Transcriptome Profiling ◽  
Rna Seq ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Use of Next Generation Sequencing and Synergy Susceptibility Testing in Diagnosis and Treatment of Carbapenem-Resistant Klebsiella pneumoniae Blood Stream Infection

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Yuetian Yu ◽  
Fupin Hu ◽  
Cheng Zhu ◽  
Erzhen Chen ◽  
Liangjing Lu ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Klebsiella Pneumoniae ◽  
Lupus Erythematosus ◽  
Susceptibility Testing ◽  
Blood Stream Infection ◽  
Blood Stream ◽  
Next Generation ◽  
Carbapenem Resistant ◽  
Appropriate Treatment ◽  
Generation Sequencing

Early diagnosis and appropriate treatment for carbapenem-resistant Klebsiella pneumoniae (CR-Kp) infection is a big challenge for clinicians due to its high mortality. Every effort has been made to improve its clinical outcomes. However, treatment according to synergy susceptibility testing has never been reported in the literature. We reported a 29-year-old systemic lupus erythematosus female with CR-Kp blood stream infection. We highlighted the identification by next generation sequencing and treatment according to synergy susceptibility testing in the case.

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