186 Protective Effects of Colchicine in an Experimental Model of Necrotizing Enterocolitis in Neonatal Rat

Introduction Necrotizing enterocolitis (NEC) is one of the major health problems of newborn period. To date, a large amount of chemicals have been tested for NEC and some showed limited beneficial effects. The research for better results still continues. This study aims to investigate the effects of quercetin (QE) on NEC treatment in rats. Methods Newborn rats were divided into control, NEC, and NEC + QE groups. In NEC and NEC + QE groups, experimental NEC was induced. NEC + QE group animals were also given QE. Weight changes of the animals were recorded, and serum total antioxidant status (TAS), total oxidant status (TOS), malondialdehyde (MDA), and glutathione (GSH) levels were measured. Histologic evaluation of the distal ileum and the terminal deoxynucleotidyl transferase dUTP nick end labeling staining were performed. Results A significant increase in the TAS levels was observed in NEC + QE group. Only NEC group exhibited significantly higher TOS and MDA levels and lower GSH levels. Rats in the NEC + QE group had better histopathology and less apoptosis than NEC group. Conclusion QE is effective in enhancing antioxidant defense mechanism, limiting oxidative stress, reducing intestinal damage, and preventing NEC development.

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The protective effects of fecal microbiota transplantation in an experimental model of necrotizing enterocolitis

Journal of Pediatric Surgery ◽

10.1016/j.jpedsurg.2018.10.045 ◽

2019 ◽

Vol 54 (8) ◽

pp. 1578-1583 ◽

Cited By ~ 4

Author(s):

Christian Prado ◽

Monique Michels ◽

Pricila Ávila ◽

Henrique Burger ◽

Maria Vitória Meller Milioli ◽

...

Keyword(s):

Experimental Model ◽

Necrotizing Enterocolitis ◽

Fecal Microbiota Transplantation ◽

Fecal Microbiota ◽

Protective Effects

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Recombinant Human Trefoil Factor 3 Ameliorates Bowel Injury: Its Anti-Inflammatory Effect on Experimental Necrotizing Enterocolitis

International Journal of Peptides ◽

10.1155/2014/634135 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 7

Author(s):

Lei Shi ◽

Pang-Hu Zhou ◽

Juan-Li Xi ◽

Hong-Gang Yu ◽

Bing-Hong Zhang

Keyword(s):

Inflammatory Response ◽

Necrotizing Enterocolitis ◽

Intestinal Inflammation ◽

Normal Group ◽

Neonatal Rat ◽

Protective Effects ◽

Trefoil Factor ◽

Morphological Studies ◽

Trefoil Factor 3 ◽

Anti Inflammatory

Aim. Recombinant human trefoil factor 3 (intestinal trefoil factor) has been suggested to be partially protective against necrotizing enterocolitis (NEC), but the mechanisms of this protection have not been defined. We investigated whether the protective effects of rhTFF3 are the result of an anti-inflammatory response. Methods. The rats were killed on day 4, the distal ileum was harvested for morphological studies and immunohistochemistry for NF-κB (p65), and the amounts of IL-1β, IL-6, and IL-10 in the intestinal tissue were measured using commercial ELISA assay kits. Results. In the neonatal NEC, IL-1β, IL-6, and IL-10 were significantly higher than in normal group. In normal group, IL-1β and IL-6 were significantly decreased, and the amount of IL-10 was markedly increased compared with NEC group. In the NEC model, immunohistochemical staining for NF-κB (p65) was demonstrated to be of a strong brown color and was distributed in the intestinal epithelium. Treatment with rhTFF3 significantly decreased the immunoreactivity of NF-κB (p65) in the NEC model. Conclusions. Intestinal inflammation was ameliorated after rhTFF3 was injected. rhTFF3 may protect against the intestinal injury of the neonatal rat NEC model by suppression of the inflammatory response.

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