Effects of epidural injections and transvaginal aspiration of ovarian follicles in heifers used repeatedly for ultrasound-guided retrieval of ova and embryo production

2002 ◽  
Vol 151 (22) ◽  
pp. 653-658 ◽  
Author(s):  
T. G. McEvoy ◽  
H. Thompson ◽  
D. F. Dolman ◽  
R. G. Watt ◽  
A. Reis ◽  
...  
Keyword(s):  
Ovarian Follicles ◽  
Ultrasound Guided ◽  
Embryo Production ◽  
Epidural Injections ◽  
Transvaginal Aspiration

Effects of delaying the LH surge and altering LH secretion on plasma hormones and embryo production in heifers superstimulated with FSH

2002 ◽  
Vol 82 (3) ◽  
pp. 357-366 ◽  
Author(s):  
P. D. Carrière ◽  
H. Kohram ◽  
C. A. Price ◽  
J. Durocher ◽  
L. A. Guilbault
Keyword(s):  
Corpora Lutea ◽  
Ultrasound Guided ◽  
Embryo Production ◽  
Lh Surge ◽  
Fourth Group ◽  
The Third ◽  
Lh Pulsatility ◽  
Transvaginal Aspiration ◽  
Plasma Hormones ◽  
Lh Secretion

Ovarian follicles were ablated by ultrasound-guided transvaginal aspiration in 20 Holstein heifers allocated to four groups (n = 5 per group). The first group (SOV) was superstimulated conventionally with FSH. In the other superstimulated groups, the LH surge was delayed by 24 h with progesterone (CIDR) and 100-μg injection of GnRH. The second group (CIDR-3.5d) received the CIDR on the second day of superstimulation. To increase LH pulsatility, the third group (CIDR-3.5d-GnRH-Pulsed) received repeated 5-μg injections of GnRH during superstimulation and the fourth group (CIDR-7d), received the CIDR with a first prostaglandin, 2 d prior to superstimulation. The number of corpora lutea (CL) and embryos was not significantly different (P > 0.1) in CIDR-3.5d compared to SOV (9.4 ± 2.0 vs. 13.8 ± 2.0 CL and 3.6 ± 1.2 vs. 5.8 ± 1.2 embryos, respectively). In the CIDR-3.5d-GnRH-Pulsed group, the 100-μg injection of GnRH failed to induce an LH surge and there were no CLs and no embryos recovered. In the CIDR-7d group, the number of CLs (5.6 ± 2.0, P < 0.05) and embryos (1.8 ± 1.2, P < 0.01) were reduced compared to SOV. In conclusion, a 24-h delay of the LH surge with a CIDR did not improve embryo production. In this delayed-surge model, increasing LH pulsatility prior to or during superstimulation resulted in a decrease in ovulation rate and embryo production. Key words: Superstimulation (FSH), GnRH, LH, CIDR, progesterone, oestradiol, embryo

58 EFFICIENCY OF OVUM PICKUP AND EMBRYO PRODUCTION IN VITRO IN CLONED CATTLE

2006 ◽  
Vol 18 (2) ◽  
pp. 137
Author(s):  
A. Lucas-Hahn ◽  
E. Lemme ◽  
K.-G. Hadeler ◽  
H.-G. Sander ◽  
H. Niemann
Keyword(s):  
Nuclear Transfer ◽  
Transvaginal Ultrasound ◽  
Statistical Significance ◽  
Blastocyst Stage ◽  
Ultrasound Guided ◽  
Embryo Production ◽  
Fetal Fibroblasts ◽  
Developmental Rates ◽  
In Vitro Embryo Production

The reproductive performance of cloned cattle was investigated by assessing the efficiency of transvaginal ultrasound-guided ovum pickup (OPU) and embryo production in vitro. Fetal fibroblasts from the endangered species, German Blackpied Cattle, had been used for nuclear transfer to produce three live cloned offspring (Lucas-Hahn et al. 2002 Theriogenology 57, 433). In the three cloned animals at 12–20 months of age, OPU was performed once per week and the total number of collected oocytes was recorded. In the case of Blondie, the procedure was terminated due to too small ovaries associated with insufficient function. Oocytes suitable for IVF were matured in vitro for 24 h and fertilized in vitro with the semen of a fertile bull. Oocytes derived from abbatoir ovaries were processed in parallel as controls. Embryos were in vitro-cultured in SOFaaBSA medium. Cleavage and developmental rates up to the morula/blastocyst stage were recorded in all groups. Statistical significance was tested using ANOVA and the Student-Newman-Keuls test. The results are presented in Table 1. Embryos from clones had lower cleavage and blastocyst rates compared to those derived from abattoir oocytes. However, results may have been confounded by potential OPU effects. Some of the blastocysts produced from Blacky (n = 5) and Paula (n = 2) were transferred to recipients. Two pregnancies resulted from the Paula transfers. The two male calves were delivered normally. After the completion of this experiment, all three cloned animals were artificially inseminated, became pregnant, delivered healthy calves, and are pregnant again at present. Further studies are needed to explore the fertility of cattle derived from somatic cloning. Table 1. OPU and in vitro embryo production in cloned cattle

Effects of superovulation on repeated ultrasound guided oocyte collection and in vitro embryo production in pregnant heifers

1997 ◽  
Vol 47 (1) ◽  
pp. 157 ◽  
Author(s):  
C.Guyader Joly ◽  
S. Ponchon ◽  
J.M. Thuard ◽  
M. Durand ◽  
M. Nibart ◽  
...  
Keyword(s):  
Ultrasound Guided ◽  
Embryo Production ◽  
Oocyte Collection ◽  
In Vitro Embryo Production

scholarly journals Ultrasound-guided Transvaginal Aspiration in the Management of Actinomyces Pelvic Abscess

1996 ◽  
Vol 4 (5) ◽  
pp. 298-300 ◽  
Author(s):  
Eyal Y. Anteby ◽  
Galiya Rahav ◽  
Josef Hanoch ◽  
Shlomo Shimonovitz ◽  
Simcha Yagel ◽  
...  
Keyword(s):  
Pelvic Abscess ◽  
Ultrasound Guided ◽  
Transvaginal Aspiration

scholarly journals Dominant follicle removal by ultrasound guided transvaginal aspiration and superovulatory response in Nellore cows

2000 ◽  
Vol 52 (1) ◽  
pp. 53-58
Author(s):  
A. Gradela ◽  
C.R. Esper ◽  
S.P.M. Matos ◽  
J.A. Lanza ◽  
L.A.G. Deragon ◽  
...  
Keyword(s):  
Physiological Significance ◽  
Dominant Follicle ◽  
Ultrasound Guided ◽  
Single Criterion ◽  
Transvaginal Aspiration ◽  
Embryonic Viability

The present study analyzed the physiological significance of the dominant follicle (DF) and its removal on the superovulatory response of 42 Nellore cows and tested the hypothesis that the number of subordinate small follicles (3-8mm) could be used as a single criterion for the evaluation of the presence or absence of a DF in the ovaries. Ultrasonographic examinations were performed two days before the beginning of superovulatory treatment (D0) and the cows divided into three groups: group without DF (NDF), group with a DF (DF) and group with an aspirated DF (ADF). The superovulatory response of the donors did not differ between groups, but the rate of embryonic viability was higher in NDF (69.40%) and ADF (68.99%) groups compared to DF (48.54%). The number of unfertilized structures did not differ between NDF and DF or between NDF and ADF groups, but was higher in DF compared to ADF. It is concluded that, in Nellore cows, the number of subordinate follicles is a safe criterion for the evaluation of the presence of a DF in the ovaries, but errors could be avoided if only 4-8mm follicles were considered, and that the presence of a DF at the beginning of superovulation reduced the embryonic viability rate and its removal by transvaginal aspiration guided by ultrasound induced embryonic viability rates similar to that observed in the absence of a DF.

scholarly journals Transvaginal ultrasound-guided oocyte aspiration for production of embryos <i>in vitro</i>

2002 ◽  
Vol 45 (1) ◽  
pp. 99-108
Author(s):  
J. A. Carter ◽  
S. Bellow ◽  
M. Meintjes ◽  
O. Perez ◽  
E. Ferguson ◽  
...  
Keyword(s):  
Transvaginal Ultrasound ◽  
Farm Animals ◽  
Embryo Research ◽  
Ultrasound Guided ◽  
Cyclic Activity ◽  
Embryo Production ◽  
Early Postpartum ◽  
In Vitro Embryo Production ◽  
Oocyte Aspiration

Abstract. reproductive potential in genetically valuable animals (BEAL et al., 1992). Now that repeatable oocyte retrieval methods are being fine-tuned, it is likely these procedures will become routinely used to obtain oocytes for further gamete and embryo research and also by seedstock producers for in vitro embryo production from farm animals in the commercial sector. The use of transvaginal ultrasound-guided oocyte aspiration and IVF procedure does offer an alternative to cattle producers who have genetically valuable cows that for some reason are unable to produce viable embryos through standard embryo collection procedures. This technology can be used on oocytes harvested from older ovulating or nonovulating cows, females with physical injuries (e.g., fractured leg) and problem cows having an abnormal cervix. Good success has been reported using IVF procedures on oocytes obtained from supplemental follicles of cows with cystic ovarian disease. With IVF the potential exists for more embryos to be produced in a shorter period of time, since the procedure can be repeated on the same cow 3 to 4 times or more a month. At this station, we are harvesting oocytes from early postpartum (< 40 days) beef and dairy cattle, before the female begins cyclic activity. The approach allows the opportunity to produce one or more extra calves from the cow before she is mated for a natural pregnancy. Currently, transvaginal ultrasound-guided oocyte aspiration is now being used to harvest valuable oocytes from minor farm animal breeds, from domestic females representing rare bloodlines, clinically infertile females and reproductively senescent cows. Research continues to find applications for this technology, including harvesting oocytes from young prepubertal heifers and early postpartum beef cows for in vitro embryo production. The use of ultrasound-guided oocyte aspiration should not be overlooked to obtain oocytes for in vitro embryo production and to aid in germplasm preservation of endangered exotic species.

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