Continuous automatic measurement of rhythms in fungal respiration using a gas chromatograph

1973 ◽  
Vol 51 (4) ◽  
pp. 701-710 ◽  
Author(s):  
Roger S. Smith

The long-term measurement of aerobic fungal respiration, both on an agar medium and on wood blocks, was possible using a gas-chromatographic technique for the detection of the carbon dioxide. This method was fully automated to analyze gas samples sequentially from eight or more growth chambers, after variable but determined time periods. It provided a precise quantitative measure of the respired carbon dioxide, presented both in the form of punched computer tape and normal printed teleprinter output. This apparatus worked continuously for several years without serious breakdown.The fungi Lentinus lepideus, Lenzites trabea, Poria monticola, and several strains of Coniophora puteana all showed a rhythm in their respiration which was not controlled by temperature or light. The magnitude and frequency of the rhythmical peaks in carbon dioxide production varied between fungi and, although there was considerable variation between different isolates of the same species, the separation of these species of fungi based on their different patterns of respiration was possible.

2011 ◽  
Vol 151 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Junhui Zhang ◽  
Xuhui Lee ◽  
Guozheng Song ◽  
Shijie Han

1986 ◽  
Vol 21 (1) ◽  
pp. 56-62
Author(s):  
Milton E. McGiffen ◽  
John V. Boyne ◽  
John R. Meyer ◽  
Sylvia M. Blankenship

A gas chromatographic technique for measurement of CO2 production in arthropods is described and used to characterize changes in CO2 production as diapause approaches in plum curculio. Carbon dioxide production was statistically compared to oxygen consumption (as measured by a constant pressure respirometer); variance was less for CO2 production, but both assays found significant differences between long-day and short-day plum curculio. Dry weight was not correlated with either CO2 production or O2 consumption. Carbon dioxide production and O2 consumption were positively correlated.


1975 ◽  
Vol 10 (1) ◽  
pp. 33-41 ◽  
Author(s):  
J. Butcher ◽  
M. Boyer ◽  
CD. Fowle

Abstract Eleven small ponds, lined with polyethylene, were used to assess the consequences of applications of *DursbanR at 0.004, 0.030, 0.100 and 1.000 ppm and AbateR at 0.025 and 0.100 ppm active ingredient. The treated ponds showed a more pronounced long-term increase in pH and dissolved oxygen and decreasing total and dissolved carbon dioxide in comparison with untreated ponds. Algal blooms were of longer duration in treated ponds than in controls. Total photosynthetic productivity was higher in treated ponds but bacterial numbers did not change significantly. Photosynthetic productivity was estimated by following the changes in total carbon dioxide.


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