Effect of either 2,4-dichlorophenoxyacetic acid or kinetin on production and composition of various fractions from extracellular polysaccharides produced by cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

Cotyledon cell suspension cultures of bush bean required 2, 4-dichlorophenoxyacetic acid (2,4-D) for growth. Kinetin was not essential but was required for optimum growth. Both of the regulators were required for optimum production of extracellular polysaccharide (EP).The two regulators had different effects upon the production of three polysaccharide fractions (two pectins and a neutral polysaccharide) isolated from the EP at various stages of the culture cycle.The neutral sugar composition of the pectin fractions from all treatments, including regulator treatments, showed considerable fluctuation during the culture cycle and could be changed markedly by regulator treatments. Changes in composition of the neutral polysaccharide were slight. As a consequence of these results it is now possible to obtain cells differing in the nature of the systems synthesizing, or controlling the synthesis of, the polysaccharides.

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Comparison of growth and extracellular polysaccharide of cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender) grown in coconut-milk medium and synthetic medium

Canadian Journal of Botany ◽

10.1139/b75-185 ◽

1975 ◽

Vol 53 (15) ◽

pp. 1542-1548 ◽

Cited By ~ 10

Author(s):

S. Mante ◽

W. G. Boll

Keyword(s):

Synthetic Medium ◽

Cell Suspension Cultures ◽

Extracellular Polysaccharide ◽

Suspension Cultures ◽

Coconut Milk ◽

Neutral Sugar ◽

Bush Bean ◽

Cotyledon Cell ◽

Neutral Sugars ◽

Culture Cycle

Growth and extracellular polysaccharide (EP) production by cotyledon suspension cultures of bush bean were compared in coconut-milk (CM) medium and synthetic medium. EP was produced at all stages of the culture cycle investigated. The same neutral sugars were present in the EP recovered from both media and the composition showed some changes during the culture cycle. Factors in coconut milk had relatively little effect on the neutral sugar composition of the EP.

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Effects of Plant Growth Regulators and Sugars on Ehretia asperula Zoll. et Mor. Cell Cultures

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-609 ◽

2021 ◽

Author(s):

P.T.M. Tram ◽

N.K. Suong ◽

L.T.T. Tien

Keyword(s):

Cell Suspension ◽

Callus Induction ◽

Malignant Tumors ◽

Single Cells ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Dichlorophenoxyacetic Acid ◽

Human Immune System ◽

Friable Callus ◽

2,4 Dichlorophenoxyacetic Acid

Background: Belonging to the Boraginacae family, Ehretia asperula Zoll. et Mor., called “Xa den”, is a precious medicinal plant also known as the “cancer tree” by the Muong ethnic group in Hoa Binh Province, Vietnam. Xa den has been demonstrated to inhibit the development of malignant tumors, reduce oxidation and enhance the human immune system. This research focused on examining friable callus induction from young stems of Ehretia asperula Zoll. et Mor. Methods: Samples of Xa den were less than two years old. Young stems with 2 to 6 leaves served as explants for callus induction. Explants placed on autoclaved B5 nutrients incubated at 25oC, in the dark. The testing factors were concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Benzyl adenine (BA), types and concentrations of sugars.Result: Friable callus was induced on B5 medium with 0.4 mg/L of 2,4-D, 0.1 mg/L of BA and 30 g/L of glucose at the highest rate (100%). Additionally, callus grew best after 5 weeks of culture weighing 0.194 g. Friable callus was used as material for cell suspension cultures. After two weeks, the Xa den cell suspension cultures contained single cells and small cell clumps. The liquid medium had changed from dark yellow to light brown.

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Extracellular polysaccharide from cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

Canadian Journal of Botany ◽

10.1139/b72-259 ◽

1972 ◽

Vol 50 (10) ◽

pp. 2031-2037 ◽

Cited By ~ 7

Author(s):

Deng-Fong Liau ◽

W. G. Boll

Keyword(s):

Cell Suspension ◽

Higher Plants ◽

Cell Suspension Cultures ◽

Extracellular Polysaccharide ◽

Cell Number ◽

Suspension Cultures ◽

Logarithmic Phase ◽

Bush Bean ◽

Culture Cycle ◽

High Yields

High yields of extracellular polysaccharide were obtained from cell suspension cultures of root, hypocotyl, and cotyledon of bush bean. Hydrolysates of the three polysaccharide samples contained the same sugars: galacturonic acid, galactose, glucose, mannose, arabinose, and xylose. The relative amounts of the six sugars were not the same in the hydrolysates from the three sources. The extracellular polysaccharide was produced at all times during the culture cycle. Semilogarithmic plots of increase in cell number, and production of extracellular polysaccharide, indicate that production per cell decreased during the logarithmic phase, and increased at the onset of the stationary phase. Production of extracellular polysaccharide, per culture and per cell, was much higher than that reported for other cell cultures of higher plants.

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