Étude de l'influence des réserves azotées dans l'estimation par 15N de la fixation symbiotique d'une plante pérenne, Trifolium pratense

1985 ◽  
Vol 63 (6) ◽  
pp. 991-994
Author(s):  
Chantal Lescure ◽  
Alain Chalamet
Keyword(s):  
Trifolium Pratense ◽  
Control Plant ◽  
Red Clover ◽  
Good Control ◽  
Dinitrogen Fixation ◽  
Dilution Technique ◽  
Trifolium Pratense L ◽  
Perennial Legumes ◽  
Kinetics Of

A 15N dilution technique is proposed to determine the role of nitrogen reserves in the plant on the estimation of dinitrogen fixation, during regrowth of perennial legumes. It is based on labelling of nitrogen compartments of the plant. Since the kinetics of utilization of nitrogen reserves of ryegrass (Lolium italicum L.) and red clover (Trifolium pratense L.) appear similar, ryegrass would be a good control plant. Despite this observation, the comparison of two methods (based on two or three sources of nitrogen) to estimate dinitrogen fixation shows the difficulty in applying the technique using 15N-labelled substrate over earlier periods of regrowth. In this case, the 15N method described for determining symbiotic dinitrogen fixation could be applied in controlled conditions.

scholarly journals The Role of Amino Acids for the In Vitro Culture of Some Species of Forage Legumes I. Trifolium pratense L

2013 ◽  
Vol 70 (1) ◽  
pp. 87-92
Author(s):  
Gabriela Maria VICAȘ ◽  
Mircea SAVATTI
Keyword(s):  
Amino Acids ◽  
In Vitro Culture ◽  
Culture Medium ◽  
Trifolium Pratense ◽  
Basal Medium ◽  
Red Clover ◽  
Forage Legumes ◽  
Trifolium Pratense L

Establishing the effect of the amino acids as additional additives to the culture medium is and will be in the future one of our concerns of interest for the in vitro culture of some plants. The present study examines the effect of the glicocol added to the LS basal medium over the embryos of the Trifolium pratense L specie cultivated in vitro. There were followed: the percentage of plant regeneration of the red clover, its multiplication capacity and the formation of the root system, and also the evolution of the callus obtained on mediums with 2,4D, BA and amino acid.

The role of medicarpin and maackiain in the response of red clover leaves to Helminthosporium carbonum, Stemphylium botryosum, and S. sarcinaeforme

1976 ◽  
Vol 54 (23) ◽  
pp. 2609-2619 ◽  
Author(s):  
Lorne J. Duczek ◽  
Verna J. Higgins
Keyword(s):  
Trifolium Pratense ◽  
Leaf Tissue ◽  
Red Clover ◽  
Stemphylium Botryosum ◽  
Inhibitory Compounds ◽  
Trifolium Pratense L ◽  
The Difference

Helminthosporium carbonum, a corn pathogen, and Stemphylium botryosum, an alfalfa pathogen, are both nonpathogenic on red clover (Trifolium pratense L.), while S. sarcinaeforme is a foliar pathogen on red clover. In clover leaves challenged with H. carbonum, medicarpin and maackiain were the only inhibitory compounds found in diffusates or in leaf tissue in a concentration sufficient to account for the inhibition of this fungus. Helminthosporium carbonum was inhibited by and could not degrade medicaipin and (or) maackiain in vitro. Both S. botryosum and S. sarcinaeforme were only slightly inhibited by these compounds in mycelial growth bioassays, and both fungi degraded medicarpin and (or) maackiain in vitro and some evidence was obtained that degradation occurred in vivo. In contrast with the relatively high amounts of medicarpin and maackiain that accumulated in leaves challenged with H. carbonum, relatively low amounts accumulated in leaves challenged with either S. botryosum or S. sarcinaeforme. The evidence suggests that the resistance of clover to H. carbonum can be accounted for by these phytoalexins; however, differences in relation to accumulation of, inhibition by, and breakdown of medicarpin and (or) maackiain were not enough to explain the difference in pathogenicity of S. botryosum and S. sarcinaeforme on red clover.

Dinitrogen fixation and nitrogen transfer among red clover cultivars

1993 ◽  
Vol 73 (4) ◽  
pp. 1047-1054 ◽  
Author(s):  
D. E. Farnham ◽  
J. R. George
Keyword(s):  
Isotope Dilution ◽  
Trifolium Pratense ◽  
Cropping Systems ◽  
Field Research ◽  
Red Clover ◽  
Dinitrogen Fixation ◽  
Nitrogen Transfer ◽  
N Transfer ◽  
Trifolium Pratense L ◽  
Dactylis Glomerata L

Red clover (Trifolium pratense L.) is an important perennial forage legume used for hay or as pasture in crop rotations. Despite its traditional usage as a source of nitrogen (N) for cropping systems, little information is available on the amounts of atmospheric dinitrogen (N2) that red clover fixes or transfers to an associated grass during long-term stands. Field research was undertaken in 1989 and 1990 to compare N2 fixation and N transfer potentials of one experimental and three common red clover cultivars seeded in binary mixtures with orchardgrass (Dactylis glomerata L.). Dinitrogen fixation and N transfer were estimated by 15N isotope dilution using orchardgrass pure stands as a reference. Over the 2-yr study, percentage legume N derived from N2 fixation ranged from 96.4 to 96.7% among the red clover cultivars. Total-season fixed-N yields in red clover herbage ranged from 72.6 to 159.2 kg ha−1. Dinitrogen fixation and fixed-N yields usually did not differ among red clover cultivars in either year. Percentage N in orchardgrass herbage derived from N2 fixation by red clover ranged from 43.7 to 70.5%. Total-season transferred-N yields in orchardgrass herbage was 16.9 kg ha−1 in 1989 and 57.8 kg ha−1 in 1990. Neither N-transfer nor transferred-N yield differed among cultivars in either year. It is concluded that, under the conditions of this study, the red clover cultivars tested generally did not differ in their abilities to fix atmospheric N2 or to transfer fixed-N to associated orchardgrass. Key words: Red clover, Trifolium pratense L., dinitrogen fixation, nitrogen transfer, isotope dilution

Control of established alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) in a no-till corn (Zea mays L.) cropping sequence

1998 ◽  
Vol 78 (1) ◽  
pp. 175-177 ◽  
Author(s):  
C. J. Swanton ◽  
K. Chandler ◽  
A. Shrestha
Keyword(s):  
Trifolium Pratense ◽  
Zea Mays L ◽  
Red Clover ◽  
No Till ◽  
Cropping Sequence ◽  
Trifolium Pratense L ◽  
Medicago Sativa L ◽  
Perennial Legumes ◽  
Fall Application ◽  
Methoxybenzoic Acid

Evaluation of several herbicides for the control of alfalfa and red clover in a legume-corn no-till system showed that spring burn-down of the legumes may be preferred to fall burn-down. Spring application gave similar or greater control of these species than fall applications. Treatments with dicamba (3,6-dichloro-2-methoxybenzoic acid) at 0.6 kg ha−1 were the most effective in controlling red clover and alfalfa. Key words: Weed control, perennial legumes, spring application, fall application

Comparisons of Floral Response of Seed Lots of Dollard Red Clover, Trifolium pratense L. 1

Crop Science ◽  
1965 ◽  
Vol 5 (5) ◽  
pp. 425-428 ◽  
Author(s):  
R. J. Bula ◽  
R. G. May ◽  
C. S. Garrison ◽  
C. M. Rincker ◽  
J. G. Dean
Keyword(s):  
Trifolium Pratense ◽  
Red Clover ◽  
Trifolium Pratense L
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