A colorimetric method for the sequence-specific recognition of double-stranded DNA on the surface of a silver-coated glass slide

2018 ◽  
Vol 96 (5) ◽  
pp. 466-470
Author(s):  
Xiaoting Guo ◽  
Jing Wang ◽  
Zhifang Zhu ◽  
Manjun Zhang ◽  
Haigang Li ◽  
...  
Keyword(s):  
Colorimetric Method ◽  
Colour Change ◽  
Glass Slide ◽  
Coated Glass ◽  
Specific Recognition ◽  
Double Stranded Dna ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Good Sequence ◽  
Coated Glass Slide

In this study, a colorimetric method for sequence-specific recognition of double-stranded DNA (dsDNA) was established on the surface of a silver-coated glass slide. Oligo-1 was assembled on the surface of a silver-coated glass slide through an Ag–S bond, and Oligo-2 as reporter was used to bind with streptavidin-horseradish peroxidase (SA–HRP). They could bind with target dsDNA that was composed of Oligo-3 and Oligo-4 on the surface of a silver-coated glass slide through triplex formation. The bound HRP could be moved into the solution by DNase I and catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Therefore, the concentration of target dsDNA could be determined with the colour change of TMB. Under the optimum conditions, the absorbance was proportional to the concentration of target dsDNA over the range of 100 pmol/L to 2.0 nmol/L, with a detection limit of 13 pmol/L. In addition, this method showed good sequence selectivity, enabling it to be further developed for the detection of other polymerase chain reaction (PCR) products.

scholarly journals Sensitive Fluorescent Sensor for Recognition of HIV-1 dsDNA by Using Glucose Oxidase and Triplex DNA

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Yubin Li ◽  
Sheng Liu ◽  
Liansheng Ling
Keyword(s):  
Glucose Oxidase ◽  
Fluorescence Intensity ◽  
Fluorescent Sensor ◽  
Specific Interaction ◽  
Triplex Dna ◽  
Coated Glass ◽  
Double Stranded Dna ◽  
Good Sequence ◽  
Oxidation Of Glucose ◽  
Hiv 1

A sensitive fluorescent sensor for sequence-specific recognition of double-stranded DNA (dsDNA) was developed on the surface of silver-coated glass slide (SCGS). Oligonucleotide-1 (Oligo-1) was designed to assemble on the surface of SCGS and act as capture DNA, and oligonucleotide-2 (Oligo-2) was designed as signal DNA. Upon addition of target HIV-1 dsDNA (Oligo-3•Oligo-4), signal DNA could bind on the surface of silver-coated glass because of the formation of C•GoC in parallel triplex DNA structure. Biotin-labeled glucose oxidase (biotin-GOx) could bind to signal DNA through the specific interaction of biotin-streptavidin, thereby GOx was attached to the surface of SCGS, which was dependent on the concentration of target HIV-1 dsDNA. GOx could catalyze the oxidation of glucose and yield H2O2, and the HPPA can be oxidized into a fluorescent product in the presence of HRP. Therefore, the concentration of target HIV-1 dsDNA could be estimated with fluorescence intensity. Under the optimum conditions, the fluorescence intensity was proportional to the concentration of target HIV-1 dsDNA over the range of 10 pM to 1000 pM, the detection limit was 3 pM. Moreover, the sensor had good sequence selectivity and practicability and might be applied for the diagnosis of HIV disease in the future.

Phosphopeptide enrichment with stable spatial coordination on a titanium dioxide coated glass slide

2009 ◽  
Vol 23 (23) ◽  
pp. 3661-3667 ◽  
Author(s):  
Susumu Y. Imanishi ◽  
Petri Kouvonen ◽  
Jan-Henrik Smått ◽  
Mikko Heikkilä ◽  
Emilia Peuhu ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Glass Slide ◽  
Phosphopeptide Enrichment ◽  
Spatial Coordination ◽  
Coated Glass ◽  
Coated Glass Slide

Measurement of photoelectron generation in a gold coated glass slide

2021 ◽  
Vol 178 ◽  
pp. 108913
Author(s):  
K.S. Almugren ◽  
S.F. Abdul Sani ◽  
E.H. Uguru ◽  
N.H. Amiera Narissa ◽  
R. Zakaria ◽  
...  
Keyword(s):  
Glass Slide ◽  
Coated Glass ◽  
Coated Glass Slide

CURRENT RECTIFICATION IN NEMATIC LIQUID CRYSTAL CELL WITH ALKANETHIOL SELF-ASSEMBLED MONOLAYER

2008 ◽  
Vol 17 (04) ◽  
pp. 495-501
Author(s):  
FENGFENG YAO ◽  
YANBO PEI ◽  
XIUDONG SUN
Keyword(s):  
Liquid Crystal ◽  
Nematic Liquid Crystal ◽  
Gold Film ◽  
Glass Slide ◽  
Self Assembled Monolayer ◽  
Coated Glass ◽  
Voltage Bias ◽  
Current Rectification ◽  
Self Assembled ◽  
Coated Glass Slide

Asymmetric current–voltage (I/V) behaviors with respect to the polarity of the voltage bias were observed in the nematic liquid crystal (NLC) cells constructed with the gold film on the silicon wafer as one substrate and an indium-tin-oxide (ITO)-coated glass slide as the other substrate. A little smaller current resulted at the positive bias corresponding to the positive voltage bias being applied to the gold film relative to the ITO-coated glass slide. When the alkanethiol self-assembled monolayer (SAM) was introduced into the above NLC cell as the aligning layer, it was able to enhance the forward current significantly, but suppress the reverse current. A current rectification ratio of ~20 was obtained at 1.5 V in the NLC cell with SAM as the aligning layer. Besides, we also characterized the effect of the optical power of 632.8 nm on the asymmetric I/V behaviors in the NLC cells without SAM and with SAM as the aligning layer.

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