Potassium bromate (KBrO3) Modulates Oxidative stress and inflammatory biomarkers in NaOH induced crohn’s colitis in Wistar rats

Potassium bromate (KBrO3) present in consumed ozonised water was recently documented to exacerbate experimental gastric ulcer. Report is however vague as regards its effects in the colon; where water reabsorption occurs. In this study, we observed the possible effects of KBrO3 on oxidative stress and inflammatory biomarkers in NaOH induced crohn’s colitis. Wistar rats (180-200g) were divided into 6 groups (n=10); 1-control, 2-untreated crohn’s colitis (induced by 1.4% NaOH; intra-rectal administration) and 3-6: crohn’s colitis treated with vitamin E, KBrO3, vitamin E+KBrO3 and sulphazalazine respectively for seven days. Body weight and stool score were monitored daily. By 3 and 7, excised colon was evaluated for ulcer scores, biochemical and histological analysis. Collected blood samples on days 3 and 7 were assayed for haematological indices using standard methods. Data were subjected to ANOVA and p ≤0.05 considered significant. Platelet/lymphocyte ratio, colonic ulcer score, , malondialdehyde and mast cells were significantly decreased while colonic sulfhydryl, Ca2+ and Na+/K+ ATPase activities were increased following KBrO3 treatment compared with crohn’s colitis untreated. Findings suggest that KBrO3 may mitigate against NaOH induced crohn’s colitis via inhibiting mast cell population, oxidative and inflammatory content but stimulating colonic sulfhydryl, Ca2+ and Na+/K+ ATPase activities.