Serum and Salivary Levels of Glucose and Urea in Pregnant and Non-pregnant Women: A Comparative Study

Background: Pregnancy is one of the most critical periods in the life of most women. Since it is necessary to be aware of the health of the mother and fetus, serum markers (i.e., glucose and urea) need to be monitored during pregnancy. A routine strategy in this area is venipuncture which is applied to measure the level of these markers. It is also a stressful procedure for pregnant women. The purpose of this study was to determine the salivary level of these markers as a stress-free method in pregnant women. Materials and Methods: The samples were collected from 30 pregnant and 30 non-pregnant fasting women. Then, the serum and salivary levels of glucose and urea were measured, analyzed, and compared by photometry. Results: Results indicated that the mean salivary glucose level was 10.2±1.4 mg/dL and 6.4±0.9 mg/ dL in non-pregnant and pregnant women, respectively. In addition, the mean serum glucose level was 106.5±5.3 mg/dL and 82.9±4.5 mg/dL in non-pregnant and pregnant women, respectively. Further, the mean salivary urea level was 37.1±3.3 mg/dL in non-pregnant women and 27.1±1.9 mg/dL in pregnant women. Moreover, the mean serum urea level was 26.9±1.9 mg/dL and 19.5±2.3 mg/dL in non-pregnant and pregnant women, respectively. Conclusion: Serum and salivary levels of glucose and urea in pregnant women were lower than those in non-pregnant women, and there was a positive correlation between serum and salivary levels. Therefore, it seems that saliva can be a substitute for serum regarding the measurement of glucose and urea levels.

Evaluation of Tear Film Urea Level as a Diagnostic Marker of Dry Eye Disease: A Pilot Study

Introduction Urea secreted in the sweat is important for skin moisture. Similarly, ocular surface moisture is maintained by the conjunctiva. Based on this, the level of urea in tear film can be used as a potential diagnostic test for dry eye disease (DED). One of the standard tests for DED is Schirmer's test (ST). The aim of this study was to compare tear film urea to values of ST. Methods Fifty patients symptomatic for DED having ST ≤ 10 mm/5 min were enrolled in the study. Fifty age- and sex-matched asymptomatic subjects with ST > 10 mm/5 min were taken as controls. All patients were subjected to an estimation of tear film urea, collected using micropipettes, and analyzed by an Erba Chem 5 semi-autoanalyzer. Based on the ST reading as per the Dry Eye Workshop 2007 (DEWS) classification, dry eye was classified as dry eye (≤ 10 mm/5 min), severe dry eye (≤ 5 mm/5 min), and very severe dry eye (≤ 2 mm/5 min). Tear film urea values were compared with ST values that were considered standard. Statistical analysis was done using Medcalc version 19.7. A p-value ≤ 0.05 was considered significant. Results The mean tear film urea levels in cases were (26.78 ± 5.70 mg/dL) significantly lower compared with controls (41.72 ± 6.86 mg/dL). The area under the receiver characteristic operator curve (AUC) for tear film urea in diagnosing DED was 0.936 (p < 0.0001) with a cutoff of ≤ 37.2 mg/dL, yielding a sensitivity of 96% and a specificity of 76%. For diagnosing severe DED, the AUC for tear film urea was 0.824 (p < 0.0001) with a cutoff value of ≤ 23.4 mg/dL, yielding a sensitivity of 60.8% and a specificity of 92.59%. For diagnosis of very severe DED, the AUC for tear film urea was 0.972 (p < 0.0001) with a cutoff value of ≤ 19.8 mg/dL, yielding a sensitivity of 100% and a specificity of 93.62%. On comparing ST values to tear film urea, the regression coefficient was 0.85 (p < 0.0001), suggesting a linear relationship between ST and tear film urea. Conclusion The study demonstrates that tear film urea can be a potential diagnostic marker for DED. The study also indicates that tear film urea level is linearly related to Schirmer's test values and provides an approximate diagnostic cutoff level for the design of future large-scale studies.

Ureum Levels Before and After Hemodialization in Renal Failure Patients

Of chronic kidney failure is a state of decreased kidney function, where the kidneys are no longer able to excrete the rest of the body's metabolism. In end-stage chronic kidney failure hemodialysis is needed to eliminate the rest of the body's metabolism that accumulates in the blood and reduce the risk of death. The effectiveness of hemodialysis can be seen from the decrease in urea levels after hemodialysis. The purpose of this study was to determine the description of urea levels before and after hemodialysis in kidney failure patients. This research method uses descriptive observational data collection with URR (Urea Reduction Ratio) involving 130 patients with kidney failure who undergo hemodialysis. This research was conducted in July and October 2019 in the laboratory unit of the Klaten Islamic Hospital. The results There was a decrease in urea levels before and after hemodialysis. Where ureum levels before hemodialysis averaged 133.19 mg / dl and after hemodialysi s averaged 39.74 mg / dl . Conclusions Urea levels in patients with renal failure before hemodialysis increase / height can be 2 times or more than normal levels, an average of 133.19 mg / dl. Increased urea level increases influenced by several factors, including sex, age, indications such as supplements, medications and diabetes mellitus. Urea levels after hemodialysis average 39.74 mg / dl resulting in a decrease in urea levels before and after hemodialysis 70,16%.

Alcohol dehydrogenase 1C (ADH1C) polymorphism is significantly associated with kidney function status in Nusa Tenggara Timur ethnicity: A cross-sectional study

Excessive alcohol consumption is harmful to many human organs, but the association with kidney function is still controversial. The disagreement in findings might be caused by ADH1C polymorphism's influence on alcohol metabolism rate. This study aims to determine the correlation between ADH1C polymorphism and kidney function status in Nusa Tenggara Timur (NTT) ethnicity, a population with highly prevalent alcohol consumption in Indonesia. We conducted a cross-sectional study of 76 subjects, who are natives of NTT, Indonesia. The genotyping of extracted DNA for ADH1C was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using restriction endonuclease SspI. Kidney function status was defined by serum urea level and estimated glomerular filtration rate (eGFR) that had been categorized according to percentiles. The correlation with the ADH1C allele was analyzed using chi-square tests. The genotype of ADH1C in NTT ethnicity was ADH1C*1/*2 (51.3%), ADH1C*2/*2 (47.4%), and ADH1C*1/*1 (1.3%). The results showed that the population had the ADH1C*2 (73.03%) and the ADH1C*1 (26.97%) allele. There was a significant association between ADH1C polymorphism and eGFR among NTT ethnicity (p=0.005) when eGFR was analyzed at the 25th percentile (74.75 mL/minute/1.73m2). However, we found no associations when eGFR was analyzed at 50th (p=0.571) and 75th (p=0.335) percentiles. The odds ratio shows that having the ADH1C*1/*2 genotype escalates the probability of declining eGFR 6.620 times compared to ADH1C*2/*2 (95% CI: 1.539-28.478), after adjusted for smoking behavior. We found no association between ADH1C polymorphism and serum urea level (p=0.123, 0.421, and 0.335). The majority of NTT ethnicity have the ADH1C*1/*2 genotype. Populations with ADH1C*1/*2 have higher odds ratio for eGFR below 74.75 mL/minute/1.73m2 than those with ADH1C*2/*2 genotype. There was no association between ADH1C polymorphism and serum urea levels.

Description of Serum Urea and Creatinine Levels Pre Hemodialysis and Post Hemodialysis at Royal Prima Hospital in Chronic Kidney Disease

Chronic Kidney Failure describes as the gradual loss of kidney functionand it is irreversible, to replace the loss of kidney function a therapy tokidney function is needed, which is hemodialysis. The objective of thisresearch is to know the levels of ureu and creatinine serum for prehemodialysis and post hemodialysis at RSU Royal Prima Medan. Themethods that are being used for this research is descriptive with 54 patientsas samples. The results of this research is loss of urea and creatinine serumlevel, with the average of urea level for pre hemodialysis is 128.11 mg/dL,and the average of urea level for post hemodialysis is 43.26 mg/dL with1,8% patient with low urea level, 50% with normal urea level, and 48.2%with high urea level. The average of creatine level for pre hemodialysis is11.56 mg/dL, and the average of creatine levels is 4.3 mg/dL, with all of thepatient still have a high creatinine levels. The conclusion is there is drop forboth urea and creatinen serum level, but the urea level for half of the patientdid not go down to normal, and for the creatinine level there is none of thepatient creatinine level that go down to normal.

Effect of urea in steamed sago waste on rumen fermentation parameters in vitro tested

This study aims to determine urea’s effect in steamed sago waste on rumen fermentation parameters in vitro testing. Sago waste was dried for two days, discarded fibre sticks, steamed for 30 minutes, cooled and dried. Weighed 250 g of steamed sago waste, added urea with levels of 0%, 2%, 4% and 6%. Weighed 0.5 g of samples per treatments, inserted in fermentor tubes, added 10 ml buffer and 10 ml of rumen fluid (1:1). Fermentor tubes are inserted in waterbath with a temperature of 39°C, flowed with CO2 gas and covered with a valved rubber cover. For NH3 and VFA testing, incubation was carried out for 4 hours, while for DMD and OMD testing, incubation was carried out for 48 hours. The data obtained were analyzed using a completely random design with four urea level treatments (0%, 2%, 4%, 6%), with five replications. The results showed that increase of urea level up to 6%, increasing (P<0.01) NH3. The increase of urea level 2%, increasing (P<0.01) VFA, DMD and OMD. The increase of urea levels 4% and 6%, not significant effect on DMD and OMD, while at urea level 6%, decreasing (P<0.01) VFA. It can be concluded that urea was added in steamed sago waste, have an optimal effect on the rumen fermentation parameters at level 2–4%.

Vacutainer serum separator sebagai alternatif tabung penampung darah pada pemeriksaan kadar ureum

ABSTRACT Serum using is preferred for urea level because it does not use anticoagulants which can interfere with activity and reaction to the results. The tubes that are widely used to collect blood into serum are vacutainer serum separator and vacutainer plain.This researche aims to determine the degree of agreement s between vacutainer serum separator and vacutainer plain usage on serum urea level result.This research was cross sectional design and hold on October 2020 with subject were taken from thirty blood samples of health analyst students which taken randomly and had no history of disease or kidney function disorder. Each student was taken 6 ml of blood drawn using a venoject with each vacutainer containing 3 ml, so we had 60 data. The data were analyzed by descriptively and inferentially using the Interclass Correlation Coefficient (ICC) statistical test. From the descriptive analysis, the difference in mean levels was 0.35 mg/dL and the ICC statistical test resulted in a degree of agreement 0.745. The data were analyzed by descriptively and inferentially using the Interclass Correlation Coefficient (ICC) statistical test. From the descriptive analysis, the difference in mean levels was 0.35 mg/dL and the ICC statistical test resulted in a degree of agreement was 0.745. The calculation of the average working time between the vacutainer serum separator and the vacutainer plain was 4 minutes 38 seconds and 35 minutes 58 seconds. The analysis concluded that the vacutainer serum separator and the vacutainer plain could be used as an alternative of blood collecting tubes for urea level testing which proved to be no significant difference in the results from this research. Keywords : Urea level, Vacutainer Serum Separator, Vacutainer Plain

COMPARISON OF HBA1C LEVEL MEASURED BY HPLC AND CAPILLARY ELECTROPHORESIS AMONG PATIENT WITH HIGH UREA

Utilization of glycated haemoglobin (HbA1c) in diagnosis and monitoring of diabetes mellitus is accepted and validated worldwide. Standardisation between various methods available is no longer an issue. However, knowledge of HbA1c interference by various haemoglobin (Hb) fractions presence in the patient’s sample must be taken into account during HbA1c analysis and interpretation. Carbamylated Hb (cHb) is one of Hb fractions, formed when Hb condensed at the N-terminal valine by cyanate derived from spontaneous decomposition of urea which usually raised in patients with renal impairment. This study aimed to compare the level of HbA1c in patient with high urea measured using High Performance Liquid Chromatography (HPLC) and Capillary Electrophoresis (CE). After analysis using the laboratory’s routine method, or HPLC, the patient’s samples with concurrent urea level of >25 mmol/L were re-analyzed within 2 hours using the comparative method or CE. A cut off cHb of 2% on HPLC considered as no interference. The mean level of urea was 31.37±5.09 mmol/L (range 25.2-43.1mmol/L). Out of 68 samples, only 24 cHb were detected by HPLC but only less than 2% and none cHb detected on CE. Correlation between HPLC and CE showed no significant different in HbA1c measurement (r= p>0.05). Therefore, we propose that both HPLC and CE can be used to determine HbA1c level in patient with high urea.

Causal Associations between Serum Urea and Cancer: A Mendelian Randomization Study

Urea is largely derived from the urea cycle reactions through hepatic detoxification of free ammonia and cleared by urination, and the serum urea level is a crucial medical indicator for measuring the kidney function in patients with nephropathy; however, investigative revelations pointing to the serum urea level as a risk factor for cancer are very scarce, and relevant studies are restricted by potential biases. We aimed to explore the causal relationships of the serum urea level with cancer development by focusing on renal cell carcinoma (RCC) using the Mendelian randomization (MR) analyses. Summary estimates were collected from the inverse-variance weighted (IVW) method based on six single nucleotide polymorphisms (SNPs). The selected SNPs related to the serum urea were obtained from a large genome-wide association study (GWAS) of 13,312 European participants. The summary statistics of RCC were also available from public databases (IARC, n = 5219 cases, n = 8011 controls). Sensitivity analyses included the weighted median and MR-Egger methods. Serum urea was inversely associated with RCC in females (effect = 1.93; 95% CI: 1.24 to 3.01; p = 0.004) but exhibited null association with RCC in males, breast cancer (BRCA) in both genders and prostate cancer (PCa) in males. Similar conclusions were also drawn from the weighted median and MR-Egger. These findings reveal an intriguing link between serum urea and cancer risks for the very first time. Without ambiguity, the serum urea is causatively related to RCC specifically in females, although the mechanism(s) by which urea is involved in RCC development remains to be experimentally/clinically investigated. Our studies may well provide novel insights for RCC diagnosis, intervention and/or therapy.