Variation of Y-chromosome DNA markers in Chinook salmon (Oncorhynchus tshawytscha) populations

2005 ◽  
Vol 62 (6) ◽  
pp. 1386-1399 ◽  
Author(s):  
R H Devlin ◽  
L Park ◽  
D M Sakhrani ◽  
J D Baker ◽  
A R Marshall ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Chinook Salmon ◽  
Dna Markers ◽  
Oncorhynchus Tshawytscha ◽  
Chromosome Structure ◽  
Single Copy ◽  
Structural Variants ◽  
Sex Marker ◽  
High Incidence ◽  
Normal Males

Two Y-chromosome DNA markers (a repetitive sequence, OtY1, and a single-copy marker, GH-Y) tightly linked to the sex-determination locus have been examined for their association with sexual development among 55 populations of Chinook salmon (Oncorhynchus tshawytscha) from the Yukon, British Columbia, Washington, Oregon, and Idaho. Normal linkage has been observed in 96.7% of 2478 individuals examined. Only five males (0.44%) were found lacking both markers (none from Canadian systems), and 14 females (1.04%) from US populations and two females (0.15%) from Canadian populations were found to possess both markers. Variants identified included weakly amplifying alleles for GH-Y and OtY1 and structural variants identified by Southern-blot analysis. The frequency of variants in males was more than 2-fold that in females, and males deficient in GH-Y were more common (3.6%) than males deficient in the repetitive OtY1 sequence (0.7%). Some individuals (of both sexes) possessed fewer copies of the OtY1 repeat than normal males, revealing molecular dynamics that alter Y-chromosome structure within and among populations. A population (Hanford Reach) previously reported as having a high incidence of females possessing the OtY1 marker, and suspected of being sex-reversed, was found to have normal sex-marker genotypes in the present study.

Corrigendum: Variation of Y-chromosome DNA markers in Chinook salmon (Oncorhynchus tshawytscha) populations

2016 ◽  
Vol 73 (1) ◽  
pp. 153-153
Author(s):  
R.H. Devlin ◽  
L. Park ◽  
D.M. Sakhrani ◽  
J.D. Baker ◽  
A.R. Marshall ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Chinook Salmon ◽  
Dna Markers ◽  
Oncorhynchus Tshawytscha

scholarly journals Heritability and Y-chromosome influence in the jack male life history of chinook salmon (Oncorhynchus tshawytscha)

Heredity ◽  
2002 ◽  
Vol 89 (4) ◽  
pp. 311-317 ◽  
Author(s):  
D D Heath ◽  
L Rankin ◽  
C A Bryden ◽  
J W Heath ◽  
J M Shrimpton
Keyword(s):  
Life History ◽  
Y Chromosome ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
History Of ◽  
Male Life History

Isolation of a Y-Chromosomal DNA Probe Capable of Determining Genetic Sex in Chinook Salmon (Oncorhynchus tshawytscha)

1991 ◽  
Vol 48 (9) ◽  
pp. 1606-1612 ◽  
Author(s):  
Robert H. Devlin ◽  
B. Kelly McNeil ◽  
T. David D. Groves ◽  
Edward M. Donaldson
Keyword(s):  
Y Chromosome ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Subtractive Hybridization ◽  
Specific Pattern ◽  
Dna Probe ◽  
Chromosomal Dna ◽  
Males And Females ◽  
Dna Fragment ◽  
Male Specific

We have used subtractive hybridization methodologies to selectively clone a DNA fragment from the Y chromosome of chinook salmon (Oncorhynchus tshawytscha). This clone specifically detects a single 8-kb Bam HI restriction fragment in Southern blots of male genomic DNA that is absent in females. This sex-specific pattern was observed in over 50 individuals from five separate chinook stocks, suggesting that the organization of this sequence is conserved in this species. The strength of the hybridization signal, and the pattern observed in multiple enzyme digests, indicates that this sequence is repeated in the male chinook genome. With long autoradiographic exposures, fainter additional sequences can also be detected both in males and females, suggesting that other similar sequences not organized in a sex-specific fashion are found on the X chromosome and/or autosomes. Examination of the segregation of this DNA sequence in families showed that the male-specific pattern segregates from father to son, providing strong evidence that the DNA probe is from the Y chromosome. The application of Y-chromosomal probes to the commercial culture of this species will simplify the synthesis of new monosex strains and allow verification of existing genotypic female, phenotypic male stocks.

Exceptions to semelparity: postmaturation survival, morphology, and energetics of male chinook salmon (Oncorhynchus tshawytscha)

1999 ◽  
Vol 56 (7) ◽  
pp. 1172-1181 ◽  
Author(s):  
M J Unwin ◽  
M T Kinnison ◽  
T P Quinn
Keyword(s):  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Gonadosomatic Index ◽  
Morphological Development ◽  
Mature Parr ◽  
In The Wild ◽  
Sib Families ◽  
Normal Males ◽  
Favorable Conditions ◽  
First Time

Between 2.1 and 6.8% of fall-run male chinook salmon (Oncorhynchus tshawytscha) reared in two New Zealand hatcheries matured as yearling parr, of similar size to immature siblings. The incidence of mature parr in 58 half-sib families ranged from 0 to 69% of the available males. Although chinook salmon are normally semelparous, about 80% of mature parr survived to mature again at age 2, and all fish held for another year matured again at age 3. All three ages produced milt that successfully fertilized eggs. Morphological development in mature parr and repeat-maturing males was consistent with that of older, first time maturing males. The gonadosomatic index for mature age-2 males was 11.7, 7.2, and 5.4% for repeat-maturing males, freshwater-reared males, and sea-run males, respectively. Muscle energy density for repeat-maturing males (4.45 kJ/g) was lower than for normal males (5.20-5.45 kJ/g) and negatively correlated with the gonadosomatic index. Although we think it unlikely that repeat maturation occurs regularly in the wild, our results indicate that under favorable conditions, chinook salmon can exhibit some iteroparous traits. We hypothesize an evolutionary continuum between semelparity and iteroparity in salmonids, primarily characterized by modifications in a few key energetic and physiological thresholds.

Y-specific sequences and polymorphisms in rainbow trout and Chinook salmon

Genome ◽  
2008 ◽  
Vol 51 (9) ◽  
pp. 739-748 ◽  
Author(s):  
Joseph P. Brunelli ◽  
Kelsey J. Wertzler ◽  
Kyle Sundin ◽  
Gary H. Thorgaard
Keyword(s):  
Rainbow Trout ◽  
Y Chromosome ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Aflp Analysis ◽  
Specific Sequence ◽  
Chromosome Marker ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Improved Methods

Improved methods for genetically sexing salmonids and for characterization of Y-chromosome homologies between species can contribute to understanding the evolution of sex chromosomes and sex-determining mechanisms. In this study we have explored 12.5 kb of Y-chromosome-specific sequence flanking the previously described OtY2 locus in Chinook salmon ( Oncorhynchus tshawytscha ) and 21 kb of homologous rainbow trout ( Oncorhynchus mykiss ) Y-chromosome-specific sequence. This is the first confirmed Y-specific sequence for rainbow trout. New Y-specific markers are described for Chinook salmon (OtY3) and rainbow trout (OmyY1), which are readily detected by PCR assays and are advantageous because they also produce autosomal control amplification products. Additionally, AFLP analysis of Chinook salmon yielded another potential Y-chromosome marker. These descriptions will facilitate genotypic sexing and should be useful for population studies of Y-chromosome polymorphisms and for future studies to characterize what appears to be a common sex-determining mechanism between these species.

Identification of the Y chromosome in chinook salmon (Oncorhynchus tshawytscha)

2001 ◽  
Vol 92 (1-2) ◽  
pp. 108-110 ◽  
Author(s):  
J. Stein ◽  
R.B. Phillips ◽  
R.H. Devlin
Keyword(s):  
Y Chromosome ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha

Systematic hexamitid (Protozoa: Diplomonadida) Infection in seawater pen-reared Chinook salmon Oncorhynchus tshawytscha

1992 ◽  
Vol 14 ◽  
pp. 81-89 ◽  
Author(s):  
ML Kent ◽  
J Ellis ◽  
JW Fournie ◽  
SC Dawe ◽  
JW Bagshaw ◽  
...  
Keyword(s):  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha

Integrating Genetic Linkage Maps With Pachytene Chromosome Structure in Maize

Genetics ◽  
2004 ◽  
Vol 166 (4) ◽  
pp. 1923-1933 ◽  
Author(s):  
Lorinda K Anderson ◽  
Naser Salameh ◽  
Hank W Bass ◽  
Lisa C Harper ◽  
W Z Cande ◽  
...  
Keyword(s):  
Genetic Linkage ◽  
Chromosome Structure ◽  
Single Copy ◽  
Chromosome 9 ◽  
Linkage Maps ◽  
Cytogenetic Map ◽  
Recombination Nodules ◽  
Novel Approach ◽  
Genetic Linkage Maps ◽  
High Degree

Abstract Genetic linkage maps reveal the order of markers based on the frequency of recombination between markers during meiosis. Because the rate of recombination varies along chromosomes, it has been difficult to relate linkage maps to chromosome structure. Here we use cytological maps of crossing over based on recombination nodules (RNs) to predict the physical position of genetic markers on each of the 10 chromosomes of maize. This is possible because (1) all 10 maize chromosomes can be individually identified from spreads of synaptonemal complexes, (2) each RN corresponds to one crossover, and (3) the frequency of RNs on defined chromosomal segments can be converted to centimorgan values. We tested our predictions for chromosome 9 using seven genetically mapped, single-copy markers that were independently mapped on pachytene chromosomes using in situ hybridization. The correlation between predicted and observed locations was very strong (r2 = 0.996), indicating a virtual 1:1 correspondence. Thus, this new, high-resolution, cytogenetic map enables one to predict the chromosomal location of any genetically mapped marker in maize with a high degree of accuracy. This novel approach can be applied to other organisms as well.

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