Genetics of Multiple Lactate Dehydrogenase Isozymes in Muscle Tissue of Lake Whitefish (Coregonus clupeaformis)

1970 ◽  
Vol 27 (6) ◽  
pp. 1115-1121 ◽  
Author(s):  
J. W. Clayton ◽  
W. G. Franzin
Keyword(s):  
Lactate Dehydrogenase ◽  
Lateral Line ◽  
White Muscle ◽  
Heart Tissue ◽  
Coregonus Clupeaformis ◽  
Lake Whitefish ◽  
Lactate Dehydrogenase Isozymes ◽  
Tissue Extracts ◽  
Fish Breeding ◽  
Ldh Isozymes

Electrophoresis of extracts of red lateral line muscle from lake whitefish (Coregonus clupeaformis) revealed two groups of lactate dehydrogenase (LDH) isozymes. In heart tissue extracts the faster moving set of isozymes predominated whereas most of the LDH in white muscle consisted of the slower set of electrophoretic isozymes.Three phenotypes of heart-type LDH isozymes were found in wild fish. Breeding experiments showed that there were two nondominant alleles at the locus coding the most anodal of the heart-type LDH subunits. The frequencies of these alleles were different in fish from two lakes.Our observations regarding the presence of discrete sets of multiple LDH isozymes in lake whitefish and the independent genetic control of isozymes within each set provide additional evidence for the probable tetraploid nature of salmonids.

Subpopulation Status of Lake Whitefish (Coregonus clupeaformis) in Lake Winnipeg

1990 ◽  
Vol 47 (8) ◽  
pp. 1484-1494 ◽  
Author(s):  
A. H. Kristofferson ◽  
J. W. Clayton
Keyword(s):  
Lactate Dehydrogenase ◽  
Coregonus Clupeaformis ◽  
Genetic Contribution ◽  
Lake Whitefish ◽  
Morphometric Measurements ◽  
Lake Winnipeg ◽  
Meristic Counts ◽  
Grand Rapids ◽  
Parent Stock ◽  
Glycerol 3 Phosphate Dehydrogenase

Spawning lake whitefish in Lake Winnipeg from Dauphin River–Lake St. Martin, Traverse Bay, Berens River–Poplar River–Big Black River and Grand Rapids and in Little Playgreen Lake are accorded subpopulation status based on morphometric measurements, meristic counts, and differences in frequencies of alleles at the muscle glycerol-3-phosphate dehydrogenase (g-3-pdh-b) locus. The frequency of the g-3-pdh-b-3 allele in samples from Dauphin River–Lake St. Martin was 0.77 compared with 0.46 for whitefish from the remainder of Lake Winnipeg and Little Playgreen Lake. Two loci for isocitrate dehydrogenase and one lactate dehydrogenase locus are also polymorphic in these fish but no significant allele frequency differences were found at these loci among the samples of spawners compared above. Hatchery-reared lake whitefish, obtained from stock in Clearwater Lake and William Lake and planted as fry or eyed eggs in Lake Winnipeg at Dauphin River and Grand Rapids, do not appear to have made a detectable genetic contribution to the local whitefish stocks, based on differences in g-3-pdh-b and idh Bβ allele frequencies among parent stock and spawners captured near the release sites.

THE ACTIVITY OF LACTATE DEHYDROGENASE ISOZYMES DURING THYROXINE-INDUCED TADPOLE METAMORPHOSIS

1966 ◽  
Vol 44 (3) ◽  
pp. 303-310 ◽  
Author(s):  
H. C. Kim ◽  
A. D'Iorio ◽  
W. K. Paik
Keyword(s):  
Lactate Dehydrogenase ◽  
Isozyme Pattern ◽  
Improved Method ◽  
Lactate Dehydrogenase Isozymes ◽  
Ldh Activity ◽  
Thyroxine Treatment ◽  
Tadpole Tail ◽  
Ldh Isozymes

A new improved method to quantitate the amount of the isozymes of lactate dehydrogenase (LDH) has been devised. The method is sensitive and very reproducible. This method has been employed for studies on LDH isozymes during thyroxine-induced tadpole metamorphosis. Only one isozyme is present in tadpole liver whereas three isozymes are present in tadpole tail and brain. Thyroxine treatment produced a decrease of the total LDH activity of tadpole liver, tail, and brain; however, the relative amount of the isozyme F3 in tail and brain is the only fraction which shows a decrease. The F1 and F2 fractions show an increase in relative amount. Thalidomide has no influence on the isozyme pattern of LDH in thyroxine-induced metamorphosis of the tadpole.

Genetics of Glycerol-3-phosphate Dehydrogenase Isozymes in White Muscle of Lake Whitefish (Coregonus clupeaformis)

1973 ◽  
Vol 30 (2) ◽  
pp. 187-193 ◽  
Author(s):  
J. W. Clayton ◽  
W. G. Franzin ◽  
D. N. Tretiak
Keyword(s):  
White Muscle ◽  
Natural Populations ◽  
Heat Inactivation ◽  
Starch Gel Electrophoresis ◽  
Coregonus Clupeaformis ◽  
Lake Whitefish ◽  
Red Muscle ◽  
Catalytically Active ◽  
Coregonus Artedii ◽  
Glycerol 3 Phosphate Dehydrogenase

Multiple isozymes of glycerol-3-phosphate dehydrogenase (L-gIycerol-3-phosphate: NAD oxidoreductase, E. C. 1.1.1.8) have been resolved by starch gel electrophoresis of extracts of muscle tissue from lake whitefish (Coregonus clupeaformis). The isozyme electropherograms show that three kinds of subunit, A, B, and C, are synthesized in red muscle and two of these, A and B, are also found in white muscle. In red muscle the subunits evidently combine to form catalytically active dimers of the following types: AA, AB, BB, BC, and CC. In white muscle only the AA, AB, and BB dimers were observed.A genetic and molecular structure model is proposed for the glycerol-3-phosphate dehydrogenase (G-3-PDH) isozymes in white muscle of lake whitefish. On the basis of two alleles for A subunits and three alleles for B subunits, the model predicts a total of 18 distinct, electrophoretic G-3-PDH phenotypes for all possible AA, AB, and BB dimeric isozymes. The model has been confirmed by the results of a breeding experiment that tested the heritability of each of the five known alleles. A difference in the rate of heat inactivation of AA and BB isozymes was also interpreted as additional evidence for the unique genetic and molecular nature of the two kinds of subunits.Surveys of natural populations of lake whitefish revealed some marked variations in the frequencies of G-3-PDH alleles in fish from different geographical areas.The cisco (Coregonus artedii) also appears to have three "b" alleles for G-3-PDH.

scholarly journals Characteristics of lactate dehydrogenase isozymes in tissue extracts of herbivorous vole, Microtus arvalis pallas.

1988 ◽  
Vol 50 (1) ◽  
pp. 287-290 ◽  
Author(s):  
Toshiro ARAI ◽  
Masashi SASAKI ◽  
Juichi TANAKA ◽  
Yoshio OKI
Keyword(s):  
Lactate Dehydrogenase ◽  
Microtus Arvalis ◽  
Lactate Dehydrogenase Isozymes ◽  
Tissue Extracts

Variants of Lactate Dehydrogenase Isozymes in Sera of Sockeye Salmon (Oncorhynchus nerka)

1969 ◽  
Vol 26 (1) ◽  
pp. 15-19 ◽  
Author(s):  
H. O. Hodgins ◽  
W. E. Ames ◽  
F. M. Utter
Keyword(s):  
British Columbia ◽  
Lactate Dehydrogenase ◽  
Sockeye Salmon ◽  
Oncorhynchus Nerka ◽  
Gulf Of Alaska ◽  
Bristol Bay ◽  
Lactate Dehydrogenase Isozymes ◽  
Skeena River ◽  
Total Body ◽  
Ldh Isozymes

Three phenotypes of lactate dehydrogenase (LDH) isozymes were found in sera of sockeye salmon (Oncorhynchus nerka), presumably representing B′B′, B′B, and BB genotypes. No association was obvious between LDH phenotype of sera and sex or total body length.Of 1006 sera from Asian, Bristol Bay, and Gulf of Alaska stocks, 826 were B′B′ and 180 were B′B or BB. Of 591 sera from Washington and British Columbia stocks, 589 were B′B′ and 2 were B′B; both of the B-allele phenotypes were found in fish captured at the Skeena River in northern British Columbia. These findings suggested that LDH isozymes should be useful in studies on ocean distribution of sockeye salmon and in characterizing certain Asian and Alaskan sockeye salmon populations.

Lactate Dehydrogenase Isozymes in Longnose and Blacknose Dace (Rhinichthys cataractae and R. atratulus) and Their Hybrid

1969 ◽  
Vol 26 (11) ◽  
pp. 3049-3053 ◽  
Author(s):  
J. W. Clayton ◽  
J. H. Gee
Keyword(s):  
Lactate Dehydrogenase ◽  
Electrophoretic Mobility ◽  
Band Pattern ◽  
Muscle Type ◽  
Muscle Lactate ◽  
Lactate Dehydrogenase Isozymes ◽  
Variant Type ◽  
Tissue Extracts ◽  
Multiple Band ◽  
Blacknose Dace

Electrophoresis of tissue extracts of both longnose and blacknose dace reveals five isozymes corresponding to tetramers of heart and muscle type subunits. The species are readily distinguished by the greater electrophoretic mobility of longnose dace isozymes that contain heart type subunits. The F1 hybrid displays the multiple band pattern expected in a fish able to produce two kinds of heart subunit. In addition a variant type of muscle lactate dehydrogenase has been detected in the longnose dace.

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