Application of Hormones in Fish Culture

1976 ◽  
Vol 33 (4) ◽  
pp. 948-958 ◽  
Author(s):  
Fumio Yamazaki

Methyltestosterone, when incorporated in the diet at a concentration of 1 ppm, accelerated the growth of goldfish (Carassius auratus), horai masu, a variant of rainbow trout (Salmo gairdneri), and kokanee (Oncorhynchus nerka). Simultaneous acceleration in the growth of the testes was also observed in some of the horai masu. Administration of 5 ppm methyltestosterone or diethylstilbestrol in the diet combined with injection of salmon gonadotropin was effective in accelerating the sexual maturation of rainbow trout. However, a concentration of 50 ppm methyltestosterone inhibited gonadal development if the treatment was initiated shortly after hatching and continued for a period of 5 mo. The fish thus treated were found to have threadlike gonads even after 3 yr. Courtship behavior and feeding behavior were also influenced by methyltestosterone treatment. The sex ratio of zebra fish (Zebrafario) and rainbow trout was altered when the administration of methyltestosterone was initiated just after hatching. This suggests a strong possibility of introducing sex reversal or mono sex culture in these important commercially raised fish. Results with the cobalt variant of rainbow trout, a fish lacking a complete pituitary and found only with immature gonads, suggest that this animal may be a promising model for future studies in fish endocrinology.




1979 ◽  
Vol 36 (10) ◽  
pp. 1174-1179 ◽  
Author(s):  
T. J. Trust ◽  
L. M. Bull ◽  
B. R. Currie ◽  
J. T. Buckley

Grass carp (Ctenopharyngodon idella), bred in Arkansas and maintained in British Columbia under defined culture conditions on pelleted diets and on aquatic weeds, were examined to determine their commensal gastrointestinal bacterial flora. Using anaerobic incubation, the total bacterial numbers cultured ranged from 6 × 104 to 4 × 108 (average 4 × 106) per g of alimentary tract plus contents. Obligately anaerobic bacteria were isolated and identified as species of Actinomyces, Bacteroides, Eubacterium, Fusobacterium, and Peptostreptococcus. This is the first description of the presence of these strictly anaerobic organisms in the gastrointestinal tract of fish. Indeed, many of the anaerobes isolated did not conform to previously described species. Members of the Genus Clostridium were also isolated by enrichment culture and appeared to be associated with a pond weed diet. A wide variety of facultative anaerobic bacteria were also isolated, with Aeromonas hydrophila predominating. The gastrointestinal tracts of goldfish (Carassius auratus) were also found to contain sizable numbers of obligate anaerobes, but the gastrointestinal tracts of hatchery-cultured rainbow trout (Salmo gairdneri) contained insignificant numbers. Key words: grass carp, anaerobic bacteria, gastrointestinal microflora, Aeromonas hydrophila, rainbow trout, goldfish



1974 ◽  
Vol 61 (2) ◽  
pp. 455-461
Author(s):  
A. H. HOUSTON ◽  
D. CYR

Significant increases in total haemoglobin concentrations, and microhaematocrit values were associated with acclimation of rainbow trout and goldfish to increased temperature. Goldfish held at 2°C were characterized by two haemoglobin components, whereas those acclimated to 20° and 35°C exhibited three. Nine haemoglobin variants were observed in trout at 2°, 10° and 18°C. The data provide evidence that both species selectively alter the concentrations of specific haemoglobin fractions during the thermoacclimatory process.





1976 ◽  
Vol 33 (6) ◽  
pp. 1403-1407 ◽  
Author(s):  
A. H. Houston ◽  
J. T. Corlett ◽  
R. J. Woods

The influence of specimen weight upon induction of and recovery from Stage I M.S. 222 (ethyl m-aminobenzoate methanesulphonate) was examined in goldfish (Carassius auratus), brook (Salvelinus fontinalis), and rainbow trout (Salmo gairdneri) exposed to various anesthetic concentration–temperature combinations. Both induction and recovery times varied inversely with weight, the influence of weight being particularly pronounced among smaller specimens. These observations are consistent with the hypothesis that attainment of "critical" internal anesthetic concentration is influenced by weight-specific variation in the relationship between gill area and extracellular phase volume.





1994 ◽  
Vol 72 (3) ◽  
pp. 423-426 ◽  
Author(s):  
Paul W. Webb ◽  
Hongbao Zhang

We measured reaction distance, escape velocity, and the apparent looming threshold (ALT) of heat-shocked goldfish (Carassius auratus) attacked by trout (Oncorhynchus mykiss). We tested fish at the acclimation temperature of 15 °C after heat-shocking prey for 2 min at temperatures ranging from 34 to 39 °C. Escape speeds were unaffected by heat shock. Reaction distance decreased from about 21 cm for fish shocked at 35 °C to about 6 cm for those shocked at 39 °C. ALT increased from 0.2 rad∙s−1 for controls to 0.4 rad∙s−1 for goldfish heat-shocked at 39 °C. The elusiveness of prey, E, was measured as the number of attacks required per prey capture. E was related to ALT as: E = 1.29 (±0.47)∙ALT−0.82(±0.25) (mean (±2 SE)). Factors that decrease responsiveness of prey have large effects on the ability of prey to avoid predators.



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