Removal of Follicle Wall Components from Ovarian Oocytes of the Brook Trout, Salvelinus fontinalis

1985 ◽  
Vol 42 (12) ◽  
pp. 2053-2058 ◽  
Author(s):  
Charles A. Lessman ◽  
Soosamma Kavumpurath ◽  
Hamid R. Habibi
Keyword(s):  
Brook Trout ◽  
Salvelinus Fontinalis ◽  
Ovarian Tissue ◽  
Meiotic Maturation ◽  
Cell Surfaces ◽  
Maturation In Vitro ◽  
Free Media ◽  
Brook Trout Oocytes ◽  
Wall Components

Fully grown brook trout (Salvelinus fontinalis) ovarian follicles, preincubated in Ca2+-free media, were dissected from other ovarian tissue by use of watchmaker forceps. Manual defolliculation and further Ca2+-free treatments were used to obtain oocytes freed of one or more follicular investments. Combinations of these treatments ultimately resulted in oocytes denuded of somatic cells as demonstrated by scanning electron microscopy. Mechanically denuded brook trout oocytes maintained the ability to undergo progestogen-induced meiotic maturation in vitro. This technique, which avoids use of enzymes that may alter cell surfaces, provides a useful system for the study of interactions between follicle wall components and the oocyte during hormonally induced meiotic maturation in vitro.

Diethylstilbestrol exposure disrupts mouse oocyte meiotic maturation in vitro through affecting spindle assembly and chromosome alignment

Chemosphere ◽  
2020 ◽  
Vol 249 ◽  
pp. 126182 ◽  
Author(s):  
Zhi-Ming Ding ◽  
Li-Ping Hua ◽  
Muhammad Jamil Ahmad ◽  
Muhammad Safdar ◽  
Fan Chen ◽  
...  
Keyword(s):  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Maturation In Vitro ◽  
Chromosome Alignment ◽  
Oocyte Meiotic Maturation

Compartmentalization of prostaglandin synthesis within the fish ovary

1991 ◽  
Vol 260 (5) ◽  
pp. R862-R865
Author(s):  
F. W. Goetz
Keyword(s):  
Arachidonic Acid ◽  
Connective Tissue ◽  
Brook Trout ◽  
Carassius Auratus ◽  
Salvelinus Fontinalis ◽  
Ovarian Tissue ◽  
Prostaglandin Synthesis ◽  
Goldfish Carassius Auratus ◽  
Significant Production ◽  
Prostaglandins E

Numerous studies have shown that the follicle walls surrounding mature vertebrate oocytes are capable of producing primary prostaglandins. However, very few studies have looked at the prostaglandin synthetic potential of other ovarian tissue components. In brook trout (Salvelinus fontinalis) and goldfish (Carassius auratus), mature follicle walls can produce prostaglandins E and F (PGE and PGF, respectively); however, it is apparent that several other tissues within the fish ovary also produce specific primary PGs. Incubation of stroma or connective tissue from brook trout and goldfish ovaries with [14C]-arachidonic acid resulted in a very significant production of PGE2, whereas small immature oocytes of both species produced primarily PGF2 alpha. While the function of the primary prostaglandins produced by tissues external to the mature oocytes is unknown, it is important to recognize that multiple sites for prostaglandin synthesis are present within the fish ovary.

Electroretinographic Determination of Spectral Sensitivity in Albino and Pigmented Brook Trout (Salvelinus fontinalis, Mitchill)

1971 ◽  
Vol 49 (12) ◽  
pp. 1030-1037 ◽  
Author(s):  
H. Kobayashi ◽  
M. A. Ali
Keyword(s):  
Absorption Spectrum ◽  
Spectral Sensitivity ◽  
Brook Trout ◽  
Salvelinus Fontinalis ◽  
Narrow Band ◽  
Visible Spectrum ◽  
The Difference

A technique for recording electroretinograms from the unpunctured eyes in situ of living, anesthetized fish is described. This technique permits the use of the same fish in a number of experiments over a period of weeks, months, or years. Using this technique the spectral sensitivity of dark-adapted (scotopic) and light-adapted (photopic) fish was measured at 13 bands of the visible spectrum. The scotopic curves of albino and pigmented trout thus obtained in the winter have their maxima around 525 nm which differ from that of the absorption spectrum of the scotopic pigment in situ and in vitro of older fish obtained in the summer. The photopic curve of the pigmented fish is a broad one with humps around 425 nm, 545 nm, and 595 nm. The albino's curve has a relatively narrow band with a peak around 630 nm and a shoulder at about 550 nm. The difference between the shapes of the two curves may be ascribed to the increase in the intensity of light of longer wavelengths within the eyeball of the albino, due to reflection from blood vessels and sclera caused by the absence of pigmentation.

Investigations on the control of in vitro spontaneous brook trout (Salvelinus fontinalis) ovulation

1986 ◽  
Vol 61 (1) ◽  
pp. 134-141 ◽  
Author(s):  
Amy K. Berndtson ◽  
Frederick W. Goetz
Keyword(s):  
Brook Trout ◽  
Salvelinus Fontinalis

Passive solute and fluid transport in brook trout (Salvelinus fontinalis) urinary bladder epithelium

1988 ◽  
Vol 66 (4) ◽  
pp. 912-918 ◽  
Author(s):  
William S. Marshall
Keyword(s):  
Hydraulic Conductivity ◽  
Urinary Bladder ◽  
Brook Trout ◽  
Fluid Transport ◽  
Salvelinus Fontinalis ◽  
Bladder Epithelium ◽  
Solute Permeability ◽  
Tight Epithelia

The passive transport of solutes across the brook trout (Salvelinus fontinalis) urinary bladder epithelium was examined in vitro in Ussing-style membrane chambers. The low transepithelial conductance (average 0.14–0.20 mS∙cm−2) and low mannitol permeability (6.9 ± 1.4 × 10−11 cm∙s−1, mean ± SE) indicate that both the transcellular and paracellular pathways have limited solute permeability. Fluid transport measurements in in vitro bag preparations indicate low hydraulic conductivity (1.6 ± 0.4 × 10−7 cm∙s−1∙atm−1; 1 atm = 101.325 kPa) and suggest that the absorbate is hyperosmotic, 5-fold more concentrated than the bathing solutions. Voltage clamping experiments with unidirectional 22Na+ and 36Cl− fluxes indicated that Na+ passive diffusion occurs primarily via a transcellular pathway, whereas the epithelium behaves as a simple resistive barrier to Cl−; thus, a diffusional portion of the Cl− flux may be paracellular. The balance of the Cl− serosa-to-mucosa flux is nonconductive and apparently represents anion exchange. Current–voltage relations were nonlinear as is typical of some tight epithelia. Bladder urine is highly hypotonic, with sodium, potassium and chloride contents of 2.00 ± 0.36, 0.76 ± 0.19 and 1.31 ± 0.20 mM, respectively. In addition to the previously demonstrated absorptive neutral NaCl active transport, present results indicate a barrier function of the urinary bladder epithelium in which hydraulic conductivity and ion and uncharged solute permeabilities are low. These characterisitics are in turn consistent with the production in vivo of a very dilute urine.

Luteinizing hormone stimulation of in vitro ovulation in brook trout (Salvelinus fontinalis) involves follicle contraction and activation of proteolytic genes

2013 ◽  
Vol 188 ◽  
pp. 175-182 ◽  
Author(s):  
Diego Crespo ◽  
Kousik Pramanick ◽  
Frederick W. Goetz ◽  
Josep V. Planas
Keyword(s):  
Luteinizing Hormone ◽  
Brook Trout ◽  
Salvelinus Fontinalis ◽  
Hormone Stimulation ◽  
Stimulation Of

Progestins inhibit murine oocyte meiotic maturation in vitro

1993 ◽  
Vol 265 (3) ◽  
pp. 231-239 ◽  
Author(s):  
C. Brent Barrett ◽  
R. Douglas Powers
Keyword(s):  
Meiotic Maturation ◽  
Maturation In Vitro ◽  
Oocyte Meiotic Maturation
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