Isolation and characterization of a set of disease resistance-gene analogs (RGAs) from wild rice, Zizania latifolia Griseb. I. Introgression, copy number lability, sequence change, and DNA methylation alteration in several rice–Zizania introgression lines

Genome ◽  
2006 ◽  
Vol 49 (2) ◽  
pp. 150-158 ◽  
Author(s):  
Yu Chen ◽  
Likun Long ◽  
Xiuyun Lin ◽  
Wanli Guo ◽  
Bao Liu
Keyword(s):  
Dna Methylation ◽  
Binding Site ◽  
Resistance Gene ◽  
Wild Rice ◽  
Copy Number ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Amino Acid Sequence Similarity ◽  
Resistance Gene Analogs ◽  
Zizania Latifolia

Eight resistance-gene analogs (RGAs) were isolated from wild rice, Zizania latifolia Griseb., by degenerate primers designed according to conserved motifs at or around the nucleotide-binding site (NBS) of known NBS-containing plant resistance genes. The 8 RGAs were classified into 6 distinct groups based on their deduced amino acid sequence similarity of 60% or greater. Gel-blot hybridization of each of the RGAs to 4 rice – Z. latifolia intro gression lines indicated an array of changes at either introgressed Zizania RGAs or, more likely, their rice homologs. The changes included dramatic increase in copy number, modification at the primary DNA sequence, and alteration in DNA methylation patterns.Key words: resistance gene analogs (RGAs), Zizania, introgression, nucleotide-binding site (NBS), leucine-rich repeat (LRR), genetic and epigenetic changes.

A superfamily of disease resistance gene analogs is located on all homoeologous chromosome groups of wheat (Triticum aestivum)

Genome ◽  
1998 ◽  
Vol 41 (6) ◽  
pp. 782-788 ◽  
Author(s):  
W Spielmeyer ◽  
M Robertson ◽  
N Collins ◽  
D Leister ◽  
P Schulze-Lefert ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Binding Site ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Resistance Gene Analogs ◽  
Leucine Rich Repeat ◽  
Homoeologous Chromosome ◽  
Entry Points

In this study, resistance gene analogs (RGAs) which were isolated from monocot crop species (wheat, barley, maize and rice) and contained conserved sequence motifs found within the nucleotide binding site - leucine rich repeat (NBS-LRR) class of resistance genes, were used to assess their distribution in the wheat genome. The RGAs showed 30-70% amino acid identity to a previously isolated monocot NBS-LRR sequence from the Cre3 locus for cereal cyst nematode (CCN) resistance in wheat. We used the RGAs as probes to identify and map loci in wheat using recombinant inbred lines of an international Triticeae mapping family. RGA loci mapped across all seven homoeologous chromosome groups of wheat. This study demonstrated that the RGA mapping approach provides potential entry points toward identifying resistance gene candidates in wheat.Key words: wheat, disease resistance genes, nucleotide binding site, leucine rich repeat, resistance gene analogs.

scholarly journals Transcriptome-wide analysis of expressed resistance gene analogs (RGAs) in mango

2020 ◽  
Author(s):  
Darlon V. Lantican ◽  
Cris Q. Cortaga ◽  
Anand Noel C. Manohar ◽  
Fe M. dela Cueva ◽  
Maria Luz J. Sison
Keyword(s):  
Binding Site ◽  
Resistance Gene ◽  
De Novo ◽  
Insect Pests ◽  
Expression Profiles ◽  
Interleukin 1 ◽  
Coiled Coil ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Resistance Gene Analogs

AbstractMango is an economically important fruit crop largely cultivated in the (sub)tropics and thus, is constantly challenged by a myriad of insect pests and diseases. Here, we identified and characterized the resistance gene analogs (RGAs) of mango from de novo assembly of transcriptomic sequences. A core RGA database of mango with 747 protein models was established and classified based on conserved domains and motifs: 53 nucleotide binding site proteins (NBS); 27 nucleotide binding site-leucine rich repeat proteins (NBS-LRR); 17 coiled-coil NBS-LRR (CNL); 2 toll/interleukin-1 receptor NBS-LRR (TNL); 29 coiled-coil NBS (CN); 4 toll/interleukin-1 receptor NBS (TN); 17 toll/interleukin-1 receptor with unknown domain (TX); 158 receptor-like proteins (RLP); 362 receptor-like kinases (RLK); 72 transmembrane coiled-coil domain protein (TM-CC), and 6 NBS-encoding proteins with other domains. The various molecular functions, biological processes, and cellular localizations of these RGAs were functionally well-annotated through gene ontology (GO) analysis, and their expression profiles across different mango varieties were also determined. Phylogenetic analysis broadly clustered the core RGAs into 6 major clades based on their domain classification, while TM-CC proteins formed subclades all across the tree. The phylogenetic results suggest highly divergent functions of the RGAs which also provide insights into the mango-pest co-evolutionary arms race. From the mango RGA transcripts, 134 unique EST-SSR loci were identified, and primers were designed targeting these potential markers. To date, this is the most comprehensive analysis of mango RGAs which offer a trove of markers for utilization in resistance breeding of mango.

Characterization of resistance gene analogs with a nucleotide binding site isolated from a triploid white poplar

Plant Biology ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 310-322 ◽  
Author(s):  
Q. Zhang ◽  
Z.-Y. Zhang ◽  
S.-Z. Lin ◽  
H.-Q. Zheng ◽  
Y.-Z. Lin ◽  
...  
Keyword(s):  
Binding Site ◽  
Resistance Gene ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Resistance Gene Analogs ◽  
White Poplar

Molecular evolution of a family of resistance gene analogs of nucleotide-binding site sequences in Solanum lycopersicum

Genetica ◽  
2011 ◽  
Vol 139 (10) ◽  
pp. 1229-1240 ◽  
Author(s):  
Pei-Chun Liao ◽  
Kuan-Hung Lin ◽  
Chin-Ling Ko ◽  
Shih-Ying Hwang
Keyword(s):  
Molecular Evolution ◽  
Binding Site ◽  
Solanum Lycopersicum ◽  
Resistance Gene ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Resistance Gene Analogs

scholarly journals Mutational Analysis of the Arabidopsis Nucleotide Binding Site–Leucine-Rich Repeat Resistance Gene RPS2

2000 ◽  
Vol 12 (12) ◽  
pp. 2541-2554 ◽  
Author(s):  
Yi Tao ◽  
Fenghua Yuan ◽  
R. Todd Leister ◽  
Frederick M. Ausubel ◽  
Fumiaki Katagiri
Keyword(s):  
Binding Site ◽  
Resistance Gene ◽  
Mutational Analysis ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Leucine Rich Repeat
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