Genetic location and biochemical characterization of eye-colour mutants from natural populations of Drosophila melanogaster

Genome ◽  
1990 ◽  
Vol 33 (2) ◽  
pp. 203-208 ◽  
Author(s):  
M. Luisa Aparisi ◽  
Carmen Nájera
Keyword(s):  
Drosophila Melanogaster ◽  
Biochemical Characterization ◽  
Natural Populations ◽  
High Variability ◽  
Genetic Location ◽  
Eye Colour ◽  
Different Seasons ◽  
Allelism Tests ◽  
Mutation And Selection

From six captures of Drosophila melanogaster carried out in three different habitats (cellar, vineyard, and pinewood) in two different seasons of the year (spring and autumn), 60 eye-colour mutations were isolated, which were reduced to 29 loci by means of allelism tests within and between populations. Forty-five of these mutations were analyzed genetically and biochemically; of these 33 turned out to be previously described mutants and mapped to a total of 17 loci. Twelve new mutants were discovered and they mapped to 12 new loci, distributed on chromosomes X, II, and III. The eye-colour mutants show large effects on the red and brown pigments. The high variability of the eye-colour loci is discussed in relation to the mutation and selection hypotheses.Key words: eye-colour mutants, variability, mapping, Drosophila melanogaster, pigment patterns.

scholarly journals Mutagenesis at Five Pteridine Pathway Loci In Drosophila melanogaster: Genetic and Biochemical Characterization

Pteridines ◽  
1993 ◽  
Vol 4 (3) ◽  
pp. 131-137
Author(s):  
J.M. Millán ◽  
C. Nájera
Keyword(s):  
Drosophila Melanogaster ◽  
Biochemical Analysis ◽  
Biochemical Characterization ◽  
Natural Populations ◽  
Ems Mutagenesis ◽  
Induced Mutants ◽  
Eye Colour

SummaryIn order to carry out a genetic and biochemical analysis of pteridin pathway eye colour loci, sixteen strains of five eye colour mutants of Drosophila melanogaster (2 dke, 7 sf. 3 se, 3 Hnr and 1 bw) from natural populations were used. Four EMS mutagenesis experiments were carried out to produce induced mutants of the same loci. 54 mutants (mosaics and completes) were obtained but only 7 (4 sf, 1 bw, 1 Hnr and I dke) could be isolated. The 40.48% of the mutations were mosaics. The percentage of mutants appeared during the four first days (85.19%) was significatively higher to the percentage of mutants appeared during the four following (14.18%). Viabilities of EMS-induced mutants were similar to that of the natural ones. For the induced mutants viability at 25°C was higher than at 16°C and heterozygotes had a higher Viability than mutant homozygotes. The low mutagenesis frequencies and the lack of some metabolites for Hnr and se mutants suggest an important role of these mutants in the pteridine pathway.

Soluble expression, purification and biochemical characterization of a C-7 cholesterol dehydrogenase from Drosophila melanogaster

Steroids ◽  
2019 ◽  
Vol 152 ◽  
pp. 108495
Author(s):  
Zhangliang Zhu ◽  
Chao Li ◽  
Xiaotao Cheng ◽  
Ying Chen ◽  
Menglu Zhu ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Biochemical Characterization ◽  
Soluble Expression

scholarly journals Variability of eye colour mutations in natural populations of Drosophila melanogaster

1988 ◽  
Vol 20 (1) ◽  
pp. 25 ◽  
Author(s):  
Carmen Nájera ◽  
JL Ménsua
Keyword(s):  
Drosophila Melanogaster ◽  
Natural Populations ◽  
Eye Colour

Genetic and biochemical characterization of little isoxanthopterin (lix), a gene controlling dihydropterin oxidase activity in Drosophila melanogaster

1991 ◽  
Vol 230 (1-2) ◽  
pp. 97-103 ◽  
Author(s):  
Francisco J. Silva ◽  
Baltasar Escriche ◽  
Eugenio Ordoño ◽  
Juan Ferré
Keyword(s):  
Drosophila Melanogaster ◽  
Oxidase Activity ◽  
Biochemical Characterization

scholarly journals Characterization of natural populations of Drosophila melanogaster with regard to the hobo system: a new hypothesis on the invasion

1997 ◽  
Vol 69 (3) ◽  
pp. 197-208 ◽  
Author(s):  
ERIC BONNIVARD ◽  
DOMINIQUE HIGUET ◽  
CLAUDE BAZIN
Keyword(s):  
Drosophila Melanogaster ◽  
Reference Strain ◽  
Natural Populations ◽  
Hybrid Dysgenesis ◽  
Molecular Characteristics ◽  
Male Recombination ◽  
System A ◽  
Genetic Level ◽  
New Hypothesis

Until now, with regard to the hobo system of hybrid dysgenesis, natural populations of Drosophila melanogaster have been investigated using only two criteria: at the molecular level, the presence or absence of XhoI fragments 2·6 kb long or smaller; and/or at the genetic level, the ability to induce gonadal dysgenesis sterility in crosses A (females of an E reference strain crossed with males under test) and A* (females under test crossed with males of an H reference strain). Recently, analyses of laboratory strains using these criteria as well as the mobilization of two reporter genes, the male recombination and the number of ‘TPE’ repeats in the S region, revealed a lack of correlation between the different dysgenic parameters themselves, and also between these parameters and the molecular characteristics of the strains. Thirteen current strains derived from world populations were therefore investigated with regard to all these dysgenic traits, to determine discriminating criteria providing a robust method of classifying natural populations and deducing the dynamics of hobo elements in these populations. We show, as in laboratory strains, a lack of correlation between the parameters studied. Therefore, the significance of each of them as well as the nature of hobo hybrid dysgenesis are discussed, to propose an analysis method of the hobo system applicable to natural populations. According to the geographical distribution of hobo activities in world populations and to the variable polymorphism of the number of ‘TPE’ repeats, we propose a new scenario for the invasion of D. melanogaster by hobo elements.

Molecular and Biochemical Characterization of the aaNATl (Dat) Locus in Drosophila melanogaster: Differential Expression of Two Gene Products

1998 ◽  
Vol 17 (7) ◽  
pp. 621-633 ◽  
Author(s):  
DANIELA BRODBECK ◽  
REMO AMHERD ◽  
PATRICK CALLAERTS ◽  
EDITH HINTERMANN ◽  
URS A. MEYER ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Differential Expression ◽  
Biochemical Characterization ◽  
Gene Products

scholarly journals CHARACTERIZATION OF ALLOZYME NULL AND LOW ACTIVITY ALLELES FROM TWO NATURAL POPULATIONS OF DROSOPHILA MELANOGASTER

Genetics ◽  
1984 ◽  
Vol 107 (2) ◽  
pp. 295-306
Author(s):  
Barbara Dickson Burkhart ◽  
Elizabeth Montgomery ◽  
Charles H Langley ◽  
Robert A Voelker
Keyword(s):  
Enzyme Activity ◽  
Drosophila Melanogaster ◽  
Transposable Element ◽  
Natural Populations ◽  
Biochemical Properties ◽  
Chromosome Rearrangements ◽  
Regulatory Sequences ◽  
Interallelic Complementation ◽  
Low Activity

ABSTRACT Null and low enzyme activity alleles recovered from two natural populations were analyzed for a number of genetic and biochemical properties. Analysis of 58 mutations at 14 loci showed that all but one allele were genetically viable and fertile, four alleles were associated with chromosome rearrangements, 28 alleles retained some enzyme activity, 13 alleles formed an active heterodimer with active alleles and five alleles showed partial interallelic complementation. Available evidence indicates that this sample includes mutations resulting from lesions in both coding and regulatory sequences. Certain mutations may be caused by transposable element insertions.

GENETIC LOCALIZATION AND BIOCHEMICAL CHARACTERIZATION OF A TRANS-ACTING REGULATORY EFFECT IN DROSOPHILA

Genetics ◽  
1983 ◽  
Vol 105 (1) ◽  
pp. 55-69
Author(s):  
Joseph J King ◽  
John F McDonald
Keyword(s):  
Drosophila Melanogaster ◽  
Biochemical Characterization ◽  
Regulatory Gene ◽  
Regulatory Effect ◽  
Protein Levels ◽  
The Third ◽  
Genetic Localization ◽  
Glycerophosphate Dehydrogenase

ABSTRACT A region-specific, trans-acting regulatory gene that alters in vivo protein levels of α-glycerophosphate dehydrogenase (α-GPDH) has been mapped to position 55.4 on the third chromosome of Drosophila melanogaster. The gene has been found to affect the in vivo stability of α-GPDH in adult thoracic tissue but has no effect on α-GPDH levels in the abdomen. Although no other thoracic proteins were found to be influenced by the locus, it appears to modify the level of one additional abdominal protein. The action of the gene over development and its possible mode of control are discussed.

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