B-chromosome and C-band heterochromatin variation in Arizona maize populations adapted to different altitudes

Genome ◽  
1990 ◽  
Vol 33 (5) ◽  
pp. 659-662 ◽  
Author(s):  
H. L. Porter ◽  
A. Lane Rayburn
Keyword(s):  
Dna Sequences ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
B Chromosome ◽  
Chromosomal Dna ◽  
Dna Amount ◽  
Maize Populations ◽  
Dna Content Variation ◽  
Band Number

The B-chromosome and C-band numbers were determined in 12 Arizona Indian maize populations. These populations were originally collected from altitudes ranging from 100 to 5300 ft (1 ft = 0.3048 m). In addition, the total nuclear DNA amounts of these populations have been observed to vary by as much as 20%. The number of B-chromosomes was not significantly correlated with altitude, C-band number, or nuclear DNA amount. C-band number was significantly correlated with both altitude and genome size. It does not appear that the amount of C-band variation can account for the large nuclear DNA variation observed in these accessions. Additional A-chromosomal DNA sequences may be involved in the nuclear DNA content variation that exists among these accessions.Key words: heterochromatin, DNA content, evolution, repeated DNA.

Nuclear DNA content variation and evolution in liverworts

2013 ◽  
Vol 68 (3) ◽  
pp. 619-627 ◽  
Author(s):  
Jillian D. Bainard ◽  
Laura L. Forrest ◽  
Bernard Goffinet ◽  
Steven G. Newmaster
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Content Variation ◽  
Dna Content Variation

scholarly journals Nuclear DNA Content Variation in Life History Phases of the Bonnemasoniaceae (Rhodophyta)

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86006
Author(s):  
Noemi Salvador Soler ◽  
Amelia Gómez Garreta ◽  
Mª Antonia Ribera Siguan ◽  
Donald F. Kapraun
Keyword(s):  
Life History ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Content Variation ◽  
Dna Content Variation

Assessment of the cellular DNA content of whole mounted mouse and human oocytes and of blastomeres containing single or multiple nuclei

Zygote ◽  
1993 ◽  
Vol 1 (1) ◽  
pp. 17-25 ◽  
Author(s):  
Nicola J. Winston ◽  
Martin H. Johnson ◽  
Peter R. Braude
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Structural Features ◽  
Chromosomal Dna ◽  
Individual Values ◽  
Mean Values ◽  
Mouse Oocytes ◽  
Cellular Dna

SummaryThe nuclear DNA content of intact, live or fixed, human and mouse oocytes and blastomeres has been measured rapidly and reliably. Chromosomal DNA has been stained with DAPI, the fluorescent emission from which has been measured photocytometrically.In vitrofertilised mouse oocytes and embryos at various stages of development were assessed for their DNA content. The mean values of 1C, 2C and 4C DNA content were clearly different, and it was possible to assign correctly individual values for DNA content to each class with 92%, 61% and 81% confidence respectively. Maintaining the cells as whole mounts allowed other morphological and structural features to be examined. When formation of multiple micronuclei was induced in mouse oocytes by their insemination in the presence of nocodazole, the additive signal from all the micronuclei in one zygote was equivalent to the expected DNA content. Application to early human blastomeres of this photocytometric technique for measurement of the total cellular DNA content revealed that multinucleated blastomeres contained 2C to 4C DNA levels, consistent with a diploid DNA content.

Nuclear DNA content variation among perennial taxa of the genus Cyanus (Asteraceae) in Central Europe and adjacent areas

2012 ◽  
Vol 298 (8) ◽  
pp. 1463-1482 ◽  
Author(s):  
Katarína Olšavská ◽  
Marián Perný ◽  
Stanislav Španiel ◽  
Barbora Šingliarová
Keyword(s):  
Central Europe ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Content Variation ◽  
Dna Content Variation

scholarly journals NUCLEAR DNA CONTENT IN BLUEBERRY DETERMINED BY FLOW CYTOMETRY

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 580e-580
Author(s):  
Rodomiro Ortiz ◽  
D.E. Costich ◽  
T.P. Meagher ◽  
N. Vorsa
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Haploid Genome ◽  
Dna Flow Cytometry ◽  
Dna Amount ◽  
Ploidy Levels ◽  
Polyploid Evolution ◽  
Haploid Genome Size

DNA flow cytometry was used to determine nuclear DNA content in diploid blueberry species, and 3x, 4x, 5x, and 6x ploidy levels. Relative fluorescence intensity of stained nuclei measured by flow cytometry was a function of the number of chromosome sets (X): Y = 3.7X – 2.3 (r2 = 95.1%). DNA flow cytometry should be useful for ploidy level determination in the seedling stage. A significant linear relationship was established between nuclear DNA content and number of chromosomes (x); DNA (pg) = 0.52 x1 (r2 = 99.8%). Based on this equation the haploid genome DNA amount (1C) was calculated as 0.62 ± 0.08 pg, with an approximate haploid genome size of 602 Mbp/1C. The results indicate that conventional polyploid evolution occured in the section Cyanococcus, genus Vaccinium: the increase in DNA was concurrent with increase in chromosome number. DNA content differences among 2x species were correlated with Nei's genetic distance estimates based on 20 isozyme markers. Most of the variation was among species (49%), with 26% between populations within species, and 25% within populations.

Nuclear DNA content variation of three Miscanthus species in China

2013 ◽  
Vol 35 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Xi Li ◽  
Die Hu ◽  
Manman Luo ◽  
Ming Zhu ◽  
Xinwei Li ◽  
...  
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Content Variation ◽  
Dna Content Variation

Genome size and genome evolution in diploid Triticeae species

Genome ◽  
2007 ◽  
Vol 50 (11) ◽  
pp. 1029-1037 ◽  
Author(s):  
T. Eilam ◽  
Y. Anikster ◽  
E. Millet ◽  
J. Manisterski ◽  
O. Sagi-Assif ◽  
...  
Keyword(s):  
Genome Size ◽  
Intraspecific Variation ◽  
Dna Content ◽  
Nuclear Dna ◽  
Diploid Species ◽  
Nuclear Dna Content ◽  
Dna Amount ◽  
Nuclear Dna Amount ◽  
Speciation Event ◽  
Close Temporal Proximity

One of the intriguing issues concerning the dynamics of plant genomes is the occurrence of intraspecific variation in nuclear DNA amount. The aim of this work was to assess the ranges of intraspecific, interspecific, and intergeneric variation in nuclear DNA content of diploid species of the tribe Triticeae (Poaceae) and to examine the relation between life form or habitat and genome size. Altogether, 438 plants representing 272 lines that belong to 22 species were analyzed. Nuclear DNA content was estimated by flow cytometry. Very small intraspecific variation in DNA amount was found between lines of Triticeae diploid species collected from different habitats or between different morphs. In contrast to the constancy in nuclear DNA amount at the intraspecific level, there are significant differences in genome size between the various diploid species. Within the genus Aegilops , the 1C DNA amount ranged from 4.84 pg in A. caudata to 7.52 pg in A. sharonensis; among genera, the 1C DNA amount ranged from 4.18 pg in Heteranthelium piliferum to 9.45 pg in Secale montanum . No evidence was found for a smaller genome size in annual, self-pollinating species relative to perennial, cross-pollinating ones. Diploids that grow in the southern part of the group’s distribution have larger genomes than those growing in other parts of the distribution. The contrast between the low variation at the intraspecific level and the high variation at the interspecific one suggests that changes in genome size originated in close temporal proximity to the speciation event, i.e., before, during, or immediately after it. The possible effects of sudden changes in genome size on speciation processes are discussed.

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