Analysis of the sex chromosomes of the Mediterranean fruit fly by microdissected DNA probes

Genome ◽  
1998 ◽  
Vol 41 (1) ◽  
pp. 74-78 ◽  
Author(s):  
Ute Willhoeft ◽  
Jutta Mueller-Navia ◽  
Gerald Franz
Keyword(s):  
Y Chromosome ◽  
X Chromosome ◽  
Sex Chromosomes ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
Oligonucleotide Primer ◽  
Mitotic Chromosomes ◽  
Degenerate Oligonucleotide ◽  
The Mediterranean

In the Mediterranean fruit fly, Ceratitis capitata, the sex-determining region maps to the long arm of the Y chromosome. DNA from this region of the Y chromosome and, for comparison, from the tip of the long arm of the X chromosome, was isolated by microdissection and amplified by degenerate oligonucleotide primer PCR (DOP-PCR). FISH of the Y-chromosomal microdissection products medY1-medY5 to mitotic chromosomes revealed hybridization signals on most of the long arm of the Y chromosome, including the male-determining region, and on the long arm of the X chromosome, as well as weaker signals on the autosomes, some of which were located in the heterochromatin next to the centromeres. The X-chromosomal microdissected probe medX1 revealed strong signals on the sex chromosomes and randomly distributed signals on the autosomes. Chromosomal in situ suppression hybridization indicates that the Y chromosome contains considerable amounts of Y-enriched and Y-specific sequences and that X-enriched sequences are present on the long arm of the X chromosome. The microdissected probes medY1, medY2, and medX1 hybridize to the sex chromosomes of two closely related species,Ceratitis rosa and Trirhithrum coffeae.

Fluorescence banding in mitotic and meiotic chromosomes of the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae)

Genome ◽  
1989 ◽  
Vol 32 (4) ◽  
pp. 580-588 ◽  
Author(s):  
D. G. Bedo
Keyword(s):  
X Chromosome ◽  
Sex Chromosomes ◽  
Silver Staining ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
Mitotic Chromosomes ◽  
C Banding ◽  
Meiotic Chromosomes ◽  
The Mediterranean

Mitotic and meiotic chromosomes of the Mediterranean fruit fly, Ceratitis capitata, were studied using three counterstain-enhanced fluorescence staining methods. The tristaining technique allowed chromomycin A3 (CMA) and distamycin – diamidinophenylindole (DA–DAPI) fluorescence to be observed on the same chromosomes. DAPI–actinomycin D (DAPI–AMD) fluorescence was also carried out. These techniques were complemented with quinacrine staining and C-banding. The results were compared with earlier data on silver staining. The sex chromosomes, particularly the X chromosome, show great banding detail with extensive longitudinal differentiation in mitotic chromosomes. GC- and AT-specific fluorescence is not found in the expected reciprocal pattern at all sites. Comparison with C-banding and silver staining shows that intense fluorescence occurs in lightly C banded regions and silver bands correspond to fluorescent bands rather than nucleolar organizers. The combination of staining data suggests that much of the X chromosome has characteristics intermediate between heterochromatin and euchromatin. Meiotic X chromosomes show much less detail and reduced fluorescence intensity but can still be easily traced throughout meiosis and spermatogenesis.Key words: fluorescence banding, sex chromosomes, Mediterranean fruit fly, Ceratitis capitata.

scholarly journals Pseudo-Y Chromosome Meiotic Drive in the Mediterranean Fruit Fly

1979 ◽  
Author(s):  
Stephen Saul ◽  
Yoram Rossler ◽  
Terrence William Lyttle
Keyword(s):  
Y Chromosome ◽  
Meiotic Drive ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
The Mediterranean

Molecular characterization and chromosomal localization of female-specific genes from the Mediterranean fruit fly Ceratitis capitata (Diptera: Tephritidae)

Genome ◽  
2005 ◽  
Vol 48 (1) ◽  
pp. 139-144 ◽  
Author(s):  
Silvia Ciolfi ◽  
Tiziana de Filippis ◽  
Cristina Torti ◽  
Anna R Malacrida ◽  
Romano Dallai
Keyword(s):  
In Situ Hybridization ◽  
Molecular Characterization ◽  
Ceratitis Capitata ◽  
Polytene Chromosomes ◽  
Fruit Fly ◽  
Accessory Gland ◽  
Mediterranean Fruit Fly ◽  
The Mediterranean ◽  
Female Specific

We report here the molecular characterization of the female-specific FST (female-specific transcript) genes from the Mediterranean fruit fly (medfly) Ceratitis capitata. A genomic clone was isolated, containing a sequence coding for FST. Nucleotide analysis of the clone showed that the gene contains a putative unique intron located in the region encoding the signal peptide. Southern blotting and in situ hybridization analysis on polytene chromosomes suggested the presence of additional genes similar to FST in the genome of the medfly. A novel cDNA clone was isolated from an accessory gland cDNA library, encoding a product that shares 98% identity with the hypothetical translational product of the previously isolated FST cDNA. The novel cDNA was therefore named FST2. The analysis of mitotic and polytene chromosomes by in situ hybridization showed that FST genes map on the left arm of the 4th chromosome of C. capitata.Key words: FST, female-specific genes, C. capitata, medfly, FISH.

Genetic and cytogenetic analysis of Y-autosome translocations in the Mediterranean fruit fly, Ceratitis capitata

Genome ◽  
1992 ◽  
Vol 35 (2) ◽  
pp. 264-272 ◽  
Author(s):  
Ph. Kerremans ◽  
E. Gencheva ◽  
G. Franz
Keyword(s):  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Mediterranean Fruit Fly ◽  
Structural Basis ◽  
Mitotic Chromosomes ◽  
Genetic Sexing ◽  
Radiation Induced ◽  
Low Levels ◽  
The Mediterranean

Radiation-induced translocations in the Mediterranean fruit fly, Ceratitis capitata, linking the Y chromosome to either autosome 3 or 4 produced pseudolinkage between sex and the mutations dark pupa (dp) and apricot eye (ap), respectively. The genetic behaviour of six new strains is described and the structural basis of five of them is determined through analysis of polytene and mitotic chromosomes. Five strains exhibited low levels of recombination; however, one strain produced a larger number than expected of aberrant, wild-type females. We provide evidence that this is the consequence of the survival of adjacent-1 segregation products until adulthood.Key words: medfly, mass rearing, genetic sexing, recombination, segregation.

Repetitive A-T rich DNA sequences from the Y chromosome of the Mediterranean fruit fly,Ceratitis capitata

Genome ◽  
2000 ◽  
Vol 43 (3) ◽  
pp. 434-438 ◽  
Author(s):  
Qin Zhou ◽  
Pia M Untalan ◽  
David S Haymer
Keyword(s):  
Y Chromosome ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Ceratitis Capitata ◽  
Pcr Amplification ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
Internal Region ◽  
Male Genome ◽  
The Mediterranean

Copies of a repetitive DNA sequence distributed over 90% of the length of the long arm of the Y chromosome of the Mediterranean fruit fly, Ceratitis capitata (medfly), have been characterized. Sequencing reveals that these repeats, ranging in size from approximately 1.3 to 1.7 kb, are A-T rich overall (67%). In most cases the repeat units appear to occur in tandemly linked arrays. The repeat copies also all contain a highly similar internal region, approximately 200 bp in length, with a more extreme A-T content bias. This internal region, designated as the AT element, exhibits an A-T content of at least 83%. This exceeds what has been described for any comparable element among invertebrates. Using primers designed from the DNA sequence, PCR amplification of an internal region encompassing the AT element also reveals that these sequences are present only in the male genome in different strains of the medfly.Key words: Y chromosome, medfly, Ceratitis capitata, AT elements, A-T content.

BANDING PATTERNS IN MITOTIC CHROMOSOMES OF THE RABBIT (ORYCTOLAGUS CUNICULUS)

1977 ◽  
Vol 19 (4) ◽  
pp. 625-632 ◽  
Author(s):  
F. P. H. Chan ◽  
F. R. Sergovich ◽  
E. L. Shaver
Keyword(s):  
Detailed Analysis ◽  
Y Chromosome ◽  
X Chromosome ◽  
Sex Chromosomes ◽  
Banding Pattern ◽  
Oryctolagus Cuniculus ◽  
Acrocentric Chromosome ◽  
Mitotic Chromosomes ◽  
Banding Patterns ◽  
Medium Length

A detailed analysis of rabbit mitotic chromosomes stained with quinacrine and Trypsin-Giemsa methods to elucidate the Q and G bands is presented. Each of the 21 pairs of autosomes can be identified unequivocally. The sex chromosomes can also be distinguished from the autosomes. The X chromosome is a medium length submetacentric with its own distinctive banding pattern. The Y chromosome is the smallest acrocentric chromosome and fluoresces with a medium intensity.

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