Fluorescence banding in mitotic and meiotic chromosomes of the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae)

Genome ◽  
1989 ◽  
Vol 32 (4) ◽  
pp. 580-588 ◽  
Author(s):  
D. G. Bedo
Keyword(s):  
X Chromosome ◽  
Sex Chromosomes ◽  
Silver Staining ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
Mitotic Chromosomes ◽  
C Banding ◽  
Meiotic Chromosomes ◽  
The Mediterranean

Mitotic and meiotic chromosomes of the Mediterranean fruit fly, Ceratitis capitata, were studied using three counterstain-enhanced fluorescence staining methods. The tristaining technique allowed chromomycin A3 (CMA) and distamycin – diamidinophenylindole (DA–DAPI) fluorescence to be observed on the same chromosomes. DAPI–actinomycin D (DAPI–AMD) fluorescence was also carried out. These techniques were complemented with quinacrine staining and C-banding. The results were compared with earlier data on silver staining. The sex chromosomes, particularly the X chromosome, show great banding detail with extensive longitudinal differentiation in mitotic chromosomes. GC- and AT-specific fluorescence is not found in the expected reciprocal pattern at all sites. Comparison with C-banding and silver staining shows that intense fluorescence occurs in lightly C banded regions and silver bands correspond to fluorescent bands rather than nucleolar organizers. The combination of staining data suggests that much of the X chromosome has characteristics intermediate between heterochromatin and euchromatin. Meiotic X chromosomes show much less detail and reduced fluorescence intensity but can still be easily traced throughout meiosis and spermatogenesis.Key words: fluorescence banding, sex chromosomes, Mediterranean fruit fly, Ceratitis capitata.

Analysis of the sex chromosomes of the Mediterranean fruit fly by microdissected DNA probes

Genome ◽  
1998 ◽  
Vol 41 (1) ◽  
pp. 74-78 ◽  
Author(s):  
Ute Willhoeft ◽  
Jutta Mueller-Navia ◽  
Gerald Franz
Keyword(s):  
Y Chromosome ◽  
X Chromosome ◽  
Sex Chromosomes ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
Oligonucleotide Primer ◽  
Mitotic Chromosomes ◽  
Degenerate Oligonucleotide ◽  
The Mediterranean

In the Mediterranean fruit fly, Ceratitis capitata, the sex-determining region maps to the long arm of the Y chromosome. DNA from this region of the Y chromosome and, for comparison, from the tip of the long arm of the X chromosome, was isolated by microdissection and amplified by degenerate oligonucleotide primer PCR (DOP-PCR). FISH of the Y-chromosomal microdissection products medY1-medY5 to mitotic chromosomes revealed hybridization signals on most of the long arm of the Y chromosome, including the male-determining region, and on the long arm of the X chromosome, as well as weaker signals on the autosomes, some of which were located in the heterochromatin next to the centromeres. The X-chromosomal microdissected probe medX1 revealed strong signals on the sex chromosomes and randomly distributed signals on the autosomes. Chromosomal in situ suppression hybridization indicates that the Y chromosome contains considerable amounts of Y-enriched and Y-specific sequences and that X-enriched sequences are present on the long arm of the X chromosome. The microdissected probes medY1, medY2, and medX1 hybridize to the sex chromosomes of two closely related species,Ceratitis rosa and Trirhithrum coffeae.

Genetic and cytogenetic analysis of Y-autosome translocations in the Mediterranean fruit fly, Ceratitis capitata

Genome ◽  
1992 ◽  
Vol 35 (2) ◽  
pp. 264-272 ◽  
Author(s):  
Ph. Kerremans ◽  
E. Gencheva ◽  
G. Franz
Keyword(s):  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Mediterranean Fruit Fly ◽  
Structural Basis ◽  
Mitotic Chromosomes ◽  
Genetic Sexing ◽  
Radiation Induced ◽  
Low Levels ◽  
The Mediterranean

Radiation-induced translocations in the Mediterranean fruit fly, Ceratitis capitata, linking the Y chromosome to either autosome 3 or 4 produced pseudolinkage between sex and the mutations dark pupa (dp) and apricot eye (ap), respectively. The genetic behaviour of six new strains is described and the structural basis of five of them is determined through analysis of polytene and mitotic chromosomes. Five strains exhibited low levels of recombination; however, one strain produced a larger number than expected of aberrant, wild-type females. We provide evidence that this is the consequence of the survival of adjacent-1 segregation products until adulthood.Key words: medfly, mass rearing, genetic sexing, recombination, segregation.

Effects of cold post-harvest treatments of sweet bell peppers on the development of the Mediterranean fruit fly ( Ceratitis capitata )

2016 ◽  
Vol 120 ◽  
pp. 16-22 ◽  
Author(s):  
Rossana Castro ◽  
Elazar Fallik ◽  
Esther Nemny-Lavy ◽  
Sharon Alkalai-Tuvia ◽  
Polychronis Rempoulakis ◽  
...  
Keyword(s):  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
Post Harvest ◽  
Bell Peppers ◽  
The Mediterranean

scholarly journals Seasonal occurrence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) in southern Syria

2016 ◽  
Vol 85 (3) ◽  
pp. 311-323 ◽  
Author(s):  
Mohammed Mansour ◽  
Fater Mohamad
Keyword(s):  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Fruit Trees ◽  
Fruit Production ◽  
Small Population ◽  
Mediterranean Fruit Fly ◽  
Diospyros Kaki ◽  
Population Fluctuations ◽  
Sampling Studies ◽  
The Mediterranean

Abstract Population fluctuations of the Mediterranean fruit fly (medfly), Ceratitis capitata, were investigated between 1999 and 2001 at several locations representing fruit production areas in the southern part of Syria (Damascus Ghota, Zabadani, Sargaiah, Rankus, Orneh and Ain Al-Arab). Medfly adults were monitored weekly all year around using Jackson traps baited with trimedlure dispensers. Larvae were also sampled in Damascus Ghota by collecting fruits from ripe or ripening fruit trees and recording the number of larvae emerged from these fruits. In addition, suspected overwintering refuges were sampled at weekly intervals during the three coldest months of the year (December – February) and the number of collected larvae was recorded. The results of trap catches and fruit sampling studies showed a similar pattern of occurrence of medfly populations in the study areas, particularly in Damascus Ghota, during the three years of the study. In Damascus Ghota, flies were caught continuously from early June to late December with some variability between years. Two distinct periods of high fly activity were observed: the first one occurred in August and the second in November with a much higher amplitude. In general, seasonal fluctuations in the pattern of occurrence were influenced by differences in temperature and abundance of preferred host fruits. Traps on fig Ficus carica and oriental persimmon Diospyros kaki trees caught the highest numbers of flies, and fruits collected from these trees showed the highest level of infestation, reaching 100% for fig fruit late in the season. Sampling fruits (in Damascus Ghota) from trees during the three coldest months of the year showed that a small population of medfly larvae was able to survive winter conditions in prickly pear Opuntia vulgaris fruit left on the trees. In the other areas of the study (Zabadani, Sargaiah, Rankus, Orneh and Ain Al-Arab), only a few flies were caught.

Polytene and mitotic chromosome analysis in Ceratitis capitata (Diptera; Tephritidae)

1986 ◽  
Vol 28 (2) ◽  
pp. 180-188 ◽  
Author(s):  
D. G. Bedo
Keyword(s):  
X Chromosome ◽  
Silver Staining ◽  
Mitotic Chromosome ◽  
Ceratitis Capitata ◽  
Fat Body ◽  
Polytene Chromosomes ◽  
Chromosome Analysis ◽  
Mitotic Chromosomes ◽  
Chromosome Separation ◽  
Ectopic Pairing

Polytene chromosomes were found in several larval and pupal tissues of the Medfly, Ceratitis capitata, during a search for chromosomes suitable for detailed cytological analysis. Well-banded highly polytene chromosomes, which could be adequately separated and spread, were found in trichogen cells of the spatulate superior orbital bristles of male pupae. These chromosomes proved suitable for full polytene analysis. Thoracic trichogen cells of both male and female pupae also contain useful polytene chromosomes, although they are considerably thinner and thus more difficult to analyze. Contrasting with those in pupal trichogen cells, the chromosomes in the salivary glands, Malphighian tubules, midgut, hindgut, and fat body of larvae and pupae were difficult to prepare because of high levels of ectopic pairing and chromosome fragmentation. In hindgut preparations partial separation of up to three chromosomes was achieved, but in all other tissues no useful chromosome separation was possible. In trichogen polytene cells, five banded chromosomes and a prominent heterochromatic network associated with a nucleolus are found. The mitotic chromosomes respond to C- and Q-banding and silver staining with considerable variation. This is especially so in the X chromosome, which displays an extensive array of bands following both Q-banding and silver staining. Comparison of Q-banded metaphase and polytene chromosomes demonstrates that the five autosomes are represented by conventional polytene chromosomes, while the sex chromosomes are contained in the heterochromatic net, most of which fluoresces strongly. This suggests that the Q-bands of the mitotic X chromosome are replicated to a greater extent than the nonfluorescent material in polytene cells. This investigation shows C. capitata to have excellent cytological material for both polytene and mitotic analysis.Key words: Ceratitis capitata, Medfly, chromosomes (polytene), banding (chromosome).

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