AGAR DIFFUSION PRECIPITATION AND COMPLEMENT FIXATION TESTS APPLIED TO THE STUDY OF THE ANTIGENIC RELATIONSHIPS BETWEEN CHROMOGENIC ACID-FAST BACTERIA AND MYCOBACTERIUM TUBERCULOSIS

1958 ◽  
Vol 4 (6) ◽  
pp. 565-570 ◽  
Author(s):  
Edith Mankiewicz
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Precipitation Reaction ◽  
Acid Fast Bacilli ◽  
Agar Diffusion ◽  
Antigenic Relationships ◽  
Diffusion Precipitation

The place of two serological techniques, the agar diffusion precipitation reaction and the complement fixation test in the classification of chromogenic acid-fast bacilli, is described. Both techniques reveal evidence of overlapping of antigens as between mycobacteria belonging to pathogenic and to saprophytic strains.

EXPERIENCES WITH A NEW ANTIGEN FOR A COMPLEMENT FIXATION TEST IN TUBERCULOSIS

1955 ◽  
Vol 1 (9) ◽  
pp. 794-798
Author(s):  
N. A. Labzoffsky
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Clinical Improvement ◽  
Ultrasonic Vibration ◽  
Antibody Titer ◽  
Guinea Pigs ◽  
Clinical Cure ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
New Method

A new method for the preparation of complement fixing antigen from Mycobacterium tuberculosis is outlined. The procedure consists of treatment of tubercle bacilli with pyridine, washing the sediment, resuspending it in saline, and finally exposing it to ultrasonic vibration. The antigen so obtained is nonviable, not anticomplementary, and appears to be specific. The results so far obtained with sera from infected guinea pigs and from human tuberculous patients indicate that complement fixing antibodies appear regularly in both. There is a suggestion that in human patients fall in antibody titer is associated with clinical improvement and disappearance with clinical cure.

Evaluation of the Complement Fixation Test in Amebiasis

1952 ◽  
Vol 21 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Elwood Buchman ◽  
Harold J. Kullman ◽  
George F. Margonis
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

IMMUNOASSAY OF GONADOTROPHINS USING COMPLEMENT FIXATION

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S113-S133 ◽  
Author(s):  
Sam Brody
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Research Work ◽  
Fixation Test ◽  
Years Of Experience ◽  
Standard Practice ◽  
Clinical Observations ◽  
Technical Procedure ◽  
Methodological Problems

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

Assessment of a complement fixation test to detect Mycoplasma hyopneumoniae infection in pigs

1984 ◽  
Vol 61 (7) ◽  
pp. 216-218
Author(s):  
L. C. LLOYD ◽  
R. T. BADMAN ◽  
J. R. ETHERIDGE ◽  
K. McKECHNIE ◽  
H. IYER
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Mycoplasma Hyopneumoniae ◽  
Fixation Test

Effect of Calcium Ion on the Kolmer Complement-Fixation Test

1954 ◽  
Vol 24 (8) ◽  
pp. 934-945 ◽  
Author(s):  
Alcor S. Browne ◽  
Martha M. Michelbacher ◽  
Edith M. Coffey
Keyword(s):  
Calcium Ion ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

scholarly journals Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51

2001 ◽  
Vol 8 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Immune Responses ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Infected Sheep ◽  
Fixation Test ◽  
Anticomplementary Activity ◽  
Serum Samples ◽  
Saline Extract ◽  
Brucella Ovis

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.

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