Production of phenylacetic acid by Bacteroides melaninogenicus ssp. intermedius serogroup C-1

1983 ◽  
Vol 29 (2) ◽  
pp. 276-279
Author(s):  
Kaethe P. Ferguson ◽  
William R. Mayberry ◽  
Dwight W. Lambe Jr.
Keyword(s):  
Gas Chromatography ◽  
Acid Production ◽  
Phenylacetic Acid ◽  
The Other ◽  
Species Status ◽  
Data Support ◽  
Methyl Derivatives ◽  
Bacteroides Gingivalis ◽  
Derivatives Of

The methyl derivatives of broth cultures of black-pigmented Bacteroides were examined by gas chromatography for production of phenylacetic acid. Two serogroups of B. melaninogenicus ssp. intermedius described by Lambe differed in the ability to produce phenylacetate. Serogroup C failed to produce phenylacetic acid while serogroup C-1 produced small amounts of phenylacetate, which contributed 2.2–5.7% to the total nonvolatile acid profile. Holdeman's newly proposed species "B. intermedius" and "B. corporis" correspond to serogroups C and C-1, respectively. These data support the elevation of the two serogroups of B. melaninogenicus ssp. intermedius to species status. Bacteroides gingivalis produced phenylacetate in significantly larger quantities than B. corporis. Bacteroides melaninogenicus ssp. melaninogenicus, "B. melaninogenicus ssp. levii," and B. asaccharolyticus did not produce phenylacetic acid. These results indicate that phenylacetic acid production may be useful in distinguishing "B. corporis" and B. gingivalis from the other black-pigmented Bacteroides.

The synthesis of the isomeric N-methyl derivatives of murexine

1981 ◽  
Vol 34 (8) ◽  
pp. 1739 ◽  
Author(s):  
CC Duke ◽  
JV Eichholzer ◽  
JK Macleod
Keyword(s):  
Imidazole Ring ◽  
The Other ◽  
Pharmacological Activities ◽  
Tautomeric Forms ◽  
Methyl Derivatives ◽  
Paramagnetic Ions ◽  
Nucella Emarginata ◽  
Derivatives Of

The two isomeric N-methyl derivatives of murexine have been synthesised by independent routes and shown to be different from an 'N- methylmurexine' reportedly isolated from the mollusc Nucella emarginata. 1H n.m.r. studies have shown a marked difference in the extent of binding to paramagnetic ions of the two N-methyl derivatives of murexine in water while pharmacological results show substantially different pharmacological activities of the two isomers. Both results can be rationalized in terms of the observed activities being associated with the presence of one or the other of the tautomeric forms of the imidazole ring.

Further studies on the differentiation of Clostridium sordellii from Clostridium bifermentans by gas chromatography

1970 ◽  
Vol 16 (11) ◽  
pp. 1071-1078 ◽  
Author(s):  
John B. Brooks ◽  
V. R. Dowell ◽  
D. C. Farshy ◽  
A. Y. Armfield
Keyword(s):  
Gas Chromatography ◽  
The Other ◽  
Gas Liquid Chromatography ◽  
Carbohydrate Composition ◽  
Whole Cells ◽  
Cultural Medium ◽  
Urease Production ◽  
Clostridium Bifermentans ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

Amines produced by 31 strains of the Clostridium bifermentans and C. sordellii groups were compared by examining trifluoroaceticanhydride derivatives of basic chloroform extracts from spent cultural medium by gas–liquid chromatography (g.l.c.). All of the urease-positive strains (16) exhibited an amine profile consistent with that of C. sordellii. On the other hand, 12 of 15 urease-negative strains produced amine g.l.c. patterns like that of C. bifermentans, and three strains produced amine patterns identical with that of C. sordellii. The carbohydrate composition of some of the strains was determined by g.l.c. of trimethylsilyl derivatives of acid-digested formamide extracts of whole cells. Two of the three urease-negative strains with amine profiles like C. sordellii had a carbohydrate composition similar to that of C. sordellii, and the other strain had a carbohydrate profile more like that of C. bifermentans. One known strain of C. bifermentans had a carbohydrate profile with characteristics of both C. bifermentans and C. sordellii. The results of this study point out the variability of urease production by C. sordellii and the value of gas chromatography in differentiating this organism from C. bifermentans.

scholarly journals Genetic variation between mice in their metabolism of coumarin and its derivatives

1978 ◽  
Vol 31 (2) ◽  
pp. 177-186 ◽  
Author(s):  
I. E. Lush ◽  
Kathryn M. Andrews
Keyword(s):  
The Other ◽  
Chromosome 7 ◽  
High Group ◽  
Substrate Specificities ◽  
Adult Females ◽  
Methyl Derivatives ◽  
The Difference ◽  
Cytochrome P 450 ◽  
Derivatives Of ◽  
Medium Group

SUMMARYAdult females from 19 strains of mice were injected with either coumarin or 7-ethoxycoumarin and the urinary excretion of the umbelliferone produced by the metabolism of these substances was measured. With the exception of C57L the strains fell into three classes as follows: high metabolizers (DBA/1 and DBA/2), medium metabolizers (CBA, 129/Rr, NZB and NZW) and low metabolizers (the other 12 strains). The difference in metabolizing ability between the medium group and the low group of strains was also evident when the 4-methyl derivatives of the same two substances were used. However with the 4-methyl derivatives there was no difference in metabolizing ability between the medium group and the high group. The results are interpreted as evidence that the gene Coh on chromosome 7 comprises two closely linked genes which determine cytochrome P-450 isozymes with different substrate specificities.

Gas chromatography of methyl derivatives of naturally occurring capsaicinoids

1987 ◽  
Vol 409 ◽  
pp. 223-233 ◽  
Author(s):  
Anna M. Krajewska ◽  
John J. Powers
Keyword(s):  
Gas Chromatography ◽  
Naturally Occurring ◽  
Methyl Derivatives ◽  
Derivatives Of

Gas chromatography of methyl derivatives of some barbiturates

1961 ◽  
Vol 6 ◽  
pp. 182-185 ◽  
Author(s):  
J.G.H. Coox ◽  
C. Riley ◽  
R.F. Nunn ◽  
D.E. Budgen
Keyword(s):  
Gas Chromatography ◽  
Methyl Derivatives ◽  
Derivatives Of

scholarly journals Electrostatic effects on disproportionation equilibria: dihalogeno and methyl derivatives of benzene

1984 ◽  
Vol 62 (11) ◽  
pp. 2337-2339 ◽  
Author(s):  
Zdeněk Friedl
Keyword(s):  
Gas Phase ◽  
The Other ◽  
Transfer Reactions ◽  
Electrostatic Energy ◽  
Benzene Derivatives ◽  
Strong Resonance ◽  
Conformational Equilibria ◽  
Proton Transfer Reactions ◽  
Methyl Derivatives ◽  
Derivatives Of

The disproportionation equilibria of benzene derivatives X—Ph—Y (X, Y = F, Cl, CH3) in the gas phase, were calculated from their enthalpies and entropies of formation at 298 K. Calculations of the electrostatic energy based on the point charges approximation method predict reasonably well the reaction enthalpies of all meta-benzene derivatives and predict nearly as well the other fluoro- and methyl-derivatives. The theory fails if strong resonance and/or steric interactions are present. The generally better ability of the electrostatic theory to treat disproportionation or conformational equilibria than the proton transfer reactions is also discussed.

Phenylacetic acid production by Bacteroides gingivalis from phenylalanine and phenylalanine-containing peptides

1983 ◽  
Vol 29 (9) ◽  
pp. 1184-1189 ◽  
Author(s):  
G. Bourgeau ◽  
D. Mayrand
Keyword(s):  
Amino Acids ◽  
Acid Production ◽  
Phenylacetic Acid ◽  
Cell Suspensions ◽  
Phenylpyruvic Acid ◽  
Phenyllactic Acid ◽  
Maximum Production ◽  
Resting Cell ◽  
Hydrocinnamic Acid ◽  
Bacteroides Gingivalis

Phenylacetic acid production and growth of Bacteroides gingivalis were directly proportional to the trypticase content of the medium. L-Phenylalanine enhanced phenylacetic acid production; 5 mg L-phenylalanine per millilitre stimulated maximum production of phenylacetic acid. Peptides (2–4 amino acids) containing L-phenylalanine also stimulated phenylacetic acid production as did phenylpyruvic acid. Resting cell suspensions of B. gingivalis also produced phenylacetic acid when incubated aerobically in the presence of L-phenylalanine and phenylpyruvic acid. Hydrocinnamic acid (3-phenylpropionic acid) and phenyllactic acid were also produced by resting cell suspensions. Our results suggest that L-phenylalanine and phenylpyruvic acid are both precursors to phenylacetic acid.

scholarly journals Synthesis and anticonvulsant activity of some 1-cyclohexylidine/ cycloheptylidine-4-substitued semicarbazide derivatives

2017 ◽  
pp. 51-56
Author(s):  
Alok Jain ◽  
Shailendra Patil ◽  
Asmita Gajbhiye
Keyword(s):  
Molecular Weight ◽  
Acetic Acid ◽  
Anticonvulsant Activity ◽  
Sodium Acetate ◽  
Hind Limb ◽  
Glacial Acetic Acid ◽  
The Other ◽  
Methyl Derivatives ◽  
Derivatives Of ◽  
Sodium Cyanate

Aryl semicarbazide derivatives are reported to possess anticonvulsant activity. On the other hand unsubstituted and small substituents (less than 3 carbon atom) containing cyclohexanones prevented both pentylenetetrazole and MES induced seizures. Similarly cycloheptanone fused with benzodiazepine and furan produced anticonvulsant compounds. Therefore looking into the above facts in the present study, we have synthesized 12 derivatives of 1-cyclohexylidine/cycloheptylidine-4-substitued semicarbazide derivatives and screened them for anticonvulsant activity. The synthesis of compounds was achieved as follows: The various para substituted (H, CH3, F,Cl,Br, I) anilines were converted to aryl ureas by reacting with sodium cyanate in the presence of glacial acetic acid. These aryl ureas and aryl semicarbazides were synthesized by allowing them to react with hydrazine hydrate. Finally the aryl semicarbazides were condensed with cyclohexanone/ cycloheptanone in the presence of sodium acetate to give title compounds. All the synthesized compounds were evaluated for anticonvulsant activity by MES method using carbamazepine as standard and it was observed that all the compounds possess anticonvulsant activity comparable to carbamazepine. Carbamazepine had shown the abolition in the hind limb extensor tonic convulsion after 2 sec. whereas few compounds i.e. 1-cyclohexylidine-4-(4-fluorophenyl) semicarbazide, 1-cycloheptylidine-4-(4- fluorophenyl) semi-carbazide and 1-cycloheptylidine-4-phenylsemicarbazide were more active than standard. Overall it was found that cycloheptyl containing compounds were more active than cyclohexyl containing compounds. The unsubstitued compounds were more active than halo derivatives i.e. electron withdrawing groups, and halo compounds were more active than methyl derivatives i.e. electron donating group (CH3). The order of activity for cyclohexanone and cycloheptanone derivatives is as follows: H >F >Cl >Br >I >CH3. Among the halo derivatives, the activity decreased with increasing molecular weight of halo substituents.

343. 1,4-Dimethoxyphthalazine and the other O- and N-methyl derivatives of phthalhydrazide

1960 ◽  
pp. 1710 ◽  
Author(s):  
J. A. Elvidge ◽  
A. P. Redman
Keyword(s):  
The Other ◽  
Methyl Derivatives ◽  
Derivatives Of

Physical Properties of TCNQ Salts with N-Methyl Derivatives of Pyridine

1982 ◽  
Vol 85 (1) ◽  
pp. 257-263 ◽  
Author(s):  
A. Graja ◽  
M. Przybylski ◽  
B. Butka ◽  
R. Swietlik
Keyword(s):  
Physical Properties ◽  
Methyl Derivatives ◽  
Derivatives Of
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