Isolation from soil of Rhizobium leguminosarum lacking symbiotic information

1989 ◽  
Vol 35 (4) ◽  
pp. 464-468 ◽  
Author(s):  
Gloria Soberón-Chávez ◽  
Rebeca Nájera
Keyword(s):  
Nitrogen Fixation ◽  
Rhizobium Leguminosarum ◽  
Plasmid Transfer ◽  
First Report ◽  
Symbiotic Plasmid

Bacteria resembling Rhizobium leguminosarum, but lacking symbiotic information, were isolated from soil of two different geographical origins. One of these bacteria belongs to a previously described Rhizobium leguminosarum bv. phaseoli somatic serogroup, is fully complemented for nodulation and nitrogen fixation by an R. leguminosarum bv. phaseoli symbiotic plasmid, and is able to compete for bean nodulation with indigenous R. leguminosarum bv. phaseoli strains. This is the first report giving evidence for persistence in soil of Rhizobium lacking symbiotic information.Key words: Rhizobium ecology, symbiotic plasmid, nodulation, plasmid transfer.

Contribution of the Symbiotic Plasmid to the Competitiveness of Rhizobium leguminosarum

Microbiology ◽  
1983 ◽  
Vol 129 (10) ◽  
pp. 2973-2977 ◽  
Author(s):  
N. J. Brewin ◽  
E. A. Wood ◽  
J. P. W. Young
Keyword(s):  
Rhizobium Leguminosarum ◽  
Symbiotic Plasmid

scholarly journals Regulation and characterization of mutants of fixABCX in Rhizobium leguminosarum.

2021 ◽  
Author(s):  
Isabel Webb ◽  
Jiabao Xu ◽  
Carmen Sanchez-Cañizares ◽  
Ramakrishnan Karunakaran ◽  
Vinoy Ramachandran ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Rhizobium Leguminosarum ◽  
Redox Balance ◽  
Rna Seq ◽  
Wild Type ◽  
Transcription Start Sites ◽  
Sym Plasmid ◽  
Microaerobic Conditions ◽  
Type Infection

Symbiosis between Rhizobium leguminosarum and Pisum sativum requires tight control of redox balance in order to maintain respiration under the microaerobic conditions required for nitrogenase, whilst still producing the eight electrons and sixteen molecules of ATP needed for nitrogen fixation. FixABCX, electron transfer flavoproteins essential for nitrogen fixation, are encoded on the Sym plasmid (pRL10), immediately upstream of nifA, which encodes the general transcriptional regulator of nitrogen fixation. There is a symbiotically-regulated NifA-dependent promoter upstream of fixA (PnifA1), as well as an additional basal constitutive promoter driving background expression of nifA (PnifA2). These were confirmed by 5’-end mapping of transcription start sites using differential (d) RNA-seq. Complementation of polar fixAB and fixX mutants (Fix- strains) confirmed expression of nifA from PnifA1 in symbiosis. Electron microscopy combined with single-cell Raman microspectroscopy characterization of fixAB mutants revealed previously unknown heterogeneity in bacteroid morphology within a single nodule. Two morphotypes of mutant fixAB bacteroids were observed. One was larger than wild-type bacteroids and contained high levels of polyhydroxy-3-butyrate, a complex energy/reductant storage product. A second bacteroid phenotype was morphologically and compositionally different and resembled wild-type infection thread cells. From these two characteristic fixAB mutant bacteroid morphotypes, inferences can be drawn on the metabolism of wild-type nitrogen-fixing bacteroids.

Quorum-sensing-regulated transcriptional initiation of plasmid transfer and replication genes in Rhizobium leguminosarum biovar viciae

Microbiology ◽  
2007 ◽  
Vol 153 (7) ◽  
pp. 2074-2082 ◽  
Author(s):  
Craig McAnulla ◽  
Anne. Edwards ◽  
Maria Sanchez-Contreras ◽  
R. Gary Sawers ◽  
J. Allan. Downie
Keyword(s):  
Quorum Sensing ◽  
Rhizobium Leguminosarum ◽  
Plasmid Transfer ◽  
Transcriptional Initiation

scholarly journals The effect of inoculation of pea plants with mycorrhizal fungi and Rhizobium on nitrogen and phosphorus assimilation

2011 ◽  
Vol 52 (No. 10) ◽  
pp. 435-440 ◽  
Author(s):  
M. Geneva ◽  
G. Zehirov ◽  
E. Djonova ◽  
N. Kaloyanova ◽  
G. Georgiev ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Mycorrhizal Fungi ◽  
Glomus Mosseae ◽  
Rhizobium Leguminosarum ◽  
Glomus Intraradices ◽  
Plant Biomass ◽  
Phosphorus Level ◽  
Nitrogen And Phosphorus ◽  
Nitrogen Fixation Activity ◽  
Fixation Activity

The study evaluated the response of pea (Pisum sativum cv. Avola) to arbuscular mycorrhizal fungi (AM) species Glomus mosseae and Glomus intraradices and Rhizobium leguminosarum bv. viceae, strain D 293, regarding the growth, photosynthesis, nodulation and nitrogen fixation activity. Pea plants were grown in a glasshouse until the flowering stage (35 days), in 4 kg plastic pots using leached cinnamonic forest soil (Chromic Luvisols – FAO) at P levels 13.2 (P1) and 39.8 (P2) mg P/kg soil. The obtained results demonstrated that the dual inoculation of pea plants significantly increased the plant biomass, photosynthetic rate, nodulation, and nitrogen fixation activity in comparison with single inoculation with Rhizobium leguminosarum bv. viceae strain D 293. On the other hand, coinoculation significantly increased the total phosphorus content in plant tissue, acid phosphatase activity and percentage of root colonization. The effectiveness of coinoculation with Rhizobium leguminosarum and Glomus mosseae was higher at the low phosphorus level while the coinoculation with Glomus intraradices appeared to be the most effective at higher phosphorus level.

scholarly journals Didymella pinodes Affects N and P Uptakes and Their Efficiencies in a Tripartite Mutualism of Pea

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 52
Author(s):  
Getinet Desalegn ◽  
Reinhard Turetschek ◽  
Stefanie Wienkoop ◽  
Hans-Peter Kaul
Keyword(s):  
Nitrogen Fixation ◽  
Grain Yield ◽  
Mycorrhizal Fungi ◽  
Rhizobium Leguminosarum ◽  
Farming System ◽  
P Uptake ◽  
Plant Health ◽  
Health Conditions ◽  
Didymella Pinodes ◽  
Microbial Symbionts

In pea (Pisum sativum L.) production, Didymella pinodes (Berk. & A. Bloxam) Petr. is the most damaging aerial pathogen globally. In two completely randomized pot experiments with four replicates, we studied the effects of D. pinodes infection interaction with three symbiotic treatments (Rhizobium leguminosarum biovar viciae, arbuscular mycorrhizal fungi (AMF) and co-inoculation of both) and a non-symbiotic control on one or two pea cultivars. Grain yield and yield components of pea, uptakes and physiological efficiencies of N and P and nitrogen fixation were recorded. The results show that there were significant interaction effects among treatments. Therefore, productivity of crops and their uptakes and efficiencies of N and P are dependent on plant health conditions, effectiveness of microbial symbionts and response of pea genotypes. For cv. Protecta inoculated with both symbionts, pathogen infection compared to healthy plants significantly enhanced P acquisition. Overall, plants inoculated with rhizobia alone had higher grain yield by 20–30% and nitrogen fixation by 20–25% than in dual symbiosis independent of plant health conditions. In conclusion, aerial pathogen, pea genotypes and microbial symbionts interactions modified N and P uptake and their efficiencies, which can lead to improving final grain yield quantity and quality in a sustainable farming system.

Sign in / Sign up

Export Citation Format

Share Document