Oil field and freshwater isolates of Shewanella putrefaciens have lipopolysaccharide polyacrylamide gel profiles characteristic of marine bacteria

1993 ◽  
Vol 39 (7) ◽  
pp. 715-717 ◽  
Author(s):  
Christopher Pickard ◽  
Julia M. Foght ◽  
Michael A. Pickard ◽  
Donald W. S. Westlake
Keyword(s):  
Marine Bacteria ◽  
Silver Staining ◽  
Gel Electrophoresis ◽  
Ferric Iron ◽  
Polyacrylamide Gel Electrophoresis ◽  
Shewanella Putrefaciens ◽  
Polyacrylamide Gel ◽  
Oil Field ◽  
Staining Procedure ◽  
Gram Negative

The lipopolysaccharide structure of oil field and freshwater isolates of bacteria that reduce ferric iron, recently classified as strains of Shewanella putrefaciens, was analyzed using polyacrylamide gel electrophoresis and a lipopolysaccharide-specific silver-staining procedure. The results demonstrate that all the oil field and freshwater isolates examined exhibited the more hydrophobic R-type lipopolysaccharide, which has been found to be characteristic of Gram-negative marine bacteria. This hydrophobic lipopolysaccharide would confer an advantage on bacteria involved in hydrocarbon degradation by assisting their association with the surface of oil droplets.Key words: lipopolysaccharide, Shewanella, aquatic bacteria, classification.

Silver staining for proteins in SDS-polyacrylamide gel electrophoresis.

1986 ◽  
Vol 30 (3) ◽  
pp. 229-238 ◽  
Author(s):  
Tsutomu Inoue ◽  
Hiroaki Asaga ◽  
Mayumi Tamura
Keyword(s):  
Silver Staining ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel

The influence of proteins on silver staining of nucleic acids following polyacrylamide gel electrophoresis

1995 ◽  
Vol 16 (1) ◽  
pp. 903-904 ◽  
Author(s):  
Friederike Hilbert ◽  
Burkhard Mayr ◽  
Fritz Lackner ◽  
Friedrich Bauer
Keyword(s):  
Nucleic Acids ◽  
Silver Staining ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel

scholarly journals Accounting for adjuvant-induced artifacts in the characterization of vaccine formulations by polyacrylamide gel electrophoresis

2017 ◽  
Vol 5 (2) ◽  
pp. 31-38 ◽  
Author(s):  
Virginie Jakob ◽  
Livia Brunner ◽  
Christophe Barnier-Quer ◽  
Molly Blust ◽  
Nicolas Collin ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Protein Characterization ◽  
Polyacrylamide Gel ◽  
Blue Staining ◽  
Staining Procedure ◽  
Water Emulsion ◽  
Oil In Water ◽  
Sds Page

Objectives: Several vaccine adjuvants comprise complex nano- or micro-particle formulations, such as oil-in-water emulsions. In order to characterize interactions and compatibility of oil-in-water emulsion adjuvants with protein antigens in vaccines, effective protein characterization methods that can accommodate potential interference from high concentrations of lipid-based particles are needed. Methods: Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a standard protein characterization technique which is affected by the presence of adjuvants such as oil-in-water emulsions. In this article, we investigate variations in SDS-PAGE methods that result in a reduction of adjuvant-induced staining artifacts. We have investigated whether the SDS method or the adjuvant composition were the reason for these artifacts and succeeded in reducing the artifacts with a modified sample preparation and different staining procedures. Results: The best results were obtained by using gold staining or silver staining instead of a Coomassie Blue staining procedure. Moreover, the replacement of the dilution buffer (20% SDS to disrupt emulsion) by alternative detergents such as Tween® 80 and Triton® X-100 removed adjuvant-induced streaking artifacts at the top of the gel. Conclusions: These methods may be useful for improving characterization approaches of antigen–adjuvant mixtures by SDS-PAGE.

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

10.3791/62319 ◽  
2021 ◽  
Author(s):  
Wells B. LaRiviere ◽  
Xiaorui Han ◽  
Kaori Oshima ◽  
Sarah A. McMurtry ◽  
Robert J. Linhardt ◽  
...  
Keyword(s):  
Silver Staining ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel

scholarly journals ROTAVIRUS INFECTION IN HUMAN AND BIRDS

1970 ◽  
Vol 7 (2) ◽  
pp. 314-319 ◽  
Author(s):  
MS Islam ◽  
MM Alam ◽  
MM Rahman ◽  
MU Ahmed
Keyword(s):  
Epidemiological Study ◽  
Silver Staining ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Zoonotic Potential ◽  
Rotavirus Infection ◽  
Stool Specimens ◽  
Rotavirus Diarrhea

An epidemiological study on rotavirus infection using 315 stool specimens from hospitalized diarrhoeic human patients and 251 fecal specimens from diarrheic birds (broiler) wear examined by RNA polyacrylamide-gel electrophoresis and silver staining (PAGE-SS) technique. Rotavirus was detected in 35.28% (111/315) stool specimens. The highest rate of rotavirus infection in human was detected in the winter (45.94%), particularly in the month of December to January (44%-51%). The rotavirus diarrhea was found slightly higher in male (35.97%) than female (34.12%). Rotavirus was detected in 13.15% (33/251) birds' fecal specimens. The birds were affected by the rotavirus, showing only the long RNA electropherotype. The antigenic studies with reference to subgroups and serotypes specificity and its zoonotic potential needs to be studied further.

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