Compétition entre souches de Rhizobiaceae: mise en évidence de différents modes d'adhésion sur les racines de soja

1993 ◽  
Vol 39 (10) ◽  
pp. 982-986 ◽  
Author(s):  
C. Dolhem-Biremon ◽  
P. Mary ◽  
R. Tailliez
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Reference Strain ◽  
Competitive Behavior ◽  
Dna Homology ◽  
Bacterial Concentration ◽  
Homology Groups ◽  
Specific Recognition ◽  
Soybean Roots ◽  
Species Specific ◽  
Root Surfaces

Adhesion of 11 Rhizobiaceae strains on soybean roots was investigated. Whether these strains were able or not to nodulate soybean, they were found to adhere to these roots. Bacterial concentration above 1 × 1010 cells∙mL−1 was necessary to saturate root surfaces. Homologues and heterologous strains were used in mixed inoculation with Bradyrhizobium japonicum G49 StrR, used as reference strain in low number. Competition for adhesion was obtained at saturating concentrations of competitive strains. Only partial inhibition (< 55%) of the G49 StrR strain adhesion in the presence of heterologous strains was obtained, suggesting the occurrence of two modes of G49 StrR strain adhesion on the host roots: (i) a nonspecific adhesion of the B. japonicum and (ii) a specific adhesion of the B. japonicum or of strain G49 StrR. With homologous strains, inhibition of G49 StrR strain adhesion indicated a different competitive behavior between the four strains used belonging to the two DNA homology groups. Thus, the existence of species-specific recognition for all strains of B. japonicum, on soybean roots, remains to be confirmed.Key words: Rhizobiaceae, soybean, competition, adhesion, root.

Genetic variability of the common nod gene in soybean bradyrhizobia isolated in Thailand and Japan

1996 ◽  
Vol 42 (12) ◽  
pp. 1209-1218 ◽  
Author(s):  
Tadashi Yokoyama ◽  
Shotaro Ando ◽  
Toshifumi Murakami ◽  
Hideo Imai
Keyword(s):  
Genetic Diversity ◽  
Bradyrhizobium Japonicum ◽  
Sequence Divergence ◽  
Gene Probe ◽  
Polymorphism Analysis ◽  
Dna Homology ◽  
Homology Groups ◽  
Phylogenetic Grouping ◽  
The Common ◽  
Bradyrhizobium Elkanii

To determine the taxonomic relationship between Thai soybean bradyrhizobia and soybean bradyrhizobia from other regions, a total of 62 Bradyrhizobium strains were isolated in Thailand. The genetic diversity of the strains was examined with reference to 46 Japanese and 15 USDA strains. The degree of sequence divergence in and around common nod gene regions of the 123 strains was estimated by restriction fragment length polymorphism analysis using the Bradyrhizobium japonicum USDA 110 common nodDYABC gene probe. The phylogenetic grouping of the strains resulted in four major clusters. Cluster 1 comprised the Japanese and USDA strains, which originated in temperate regions, whereas clusters 3 and 4 comprised the tropical Thai strains. Cluster 1 strains comprised the DNA homology groups I and Ia, and hence, were classified as B. japonicum. Cluster 2 strains were in the DNA homology group II, and hence, were classified as Bradyrhizobium elkanii. Clusters 3 and 4 strains, however, did not correspond to any known DNA homology groups. These results indicate that Thai soybean bradyrhizobia are distantly related to B. japonicum and B. elkanii.Key words: Bradyrhizobium japonicum, Bradyrhizobium elkanii, common nod gene, RFLP, genetic diversity.

scholarly journals Specific Recognition and Cleavage of Galectin-3 byLeishmania majorthrough Species-specific Polygalactose Epitope

2002 ◽  
Vol 277 (20) ◽  
pp. 17663-17670 ◽  
Author(s):  
Isabelle Pelletier ◽  
Sachiko Sato
Keyword(s):  
Specific Recognition ◽  
Galectin 3 ◽  
Species Specific

Characterisation of a tetrasaccharide released on mild acid hydrolysis of LPS from two rough strains of Shewanella species representing different DNA homology groups

2004 ◽  
Vol 339 (6) ◽  
pp. 1185-1188 ◽  
Author(s):  
Anne L Moule ◽  
Lesley Galbraith ◽  
Andrew D Cox ◽  
Stephen G Wilkinson
Keyword(s):  
Acid Hydrolysis ◽  
Dna Homology ◽  
Mild Acid Hydrolysis ◽  
Homology Groups ◽  
Shewanella Species ◽  
Hydrolysis Of ◽  
Mild Acid

Differential characteristics of catalase-positive Campylobacters correlated with DNA homology groups

1984 ◽  
Vol 30 (7) ◽  
pp. 938-951 ◽  
Author(s):  
R. Martin Roop II ◽  
Robert M. Smibert ◽  
John L. Johnson ◽  
Noel R. Krieg
Keyword(s):  
Minimal Medium ◽  
Nitrite Reduction ◽  
Anaerobic Growth ◽  
Dna Homology ◽  
Acid Growth ◽  
Homology Groups ◽  
Air Atmosphere ◽  
Subspecies Level ◽  
Hydrogen Sulfide Production ◽  
Reliable Differentiation

Eighty-four strains of catalase-positive campylobacters could be placed into seven distinct DNA homology groups (species), corresponding to Campylobacter fetus, "C. hyointestinalis," C. jejuni, C. coli, "C. laridis," "C. fecalis," and aerotolerant Campylobacters. The biochemical and physiological characteristics of the strains were examined for their correlation with the homology groups. The characterization tests that provided the most reliable differentiation at the species and subspecies level were growth at 25 and 42 °C, sensitivity to cephalothin and nalidixic acid, growth in semisolid media containing 1% glycine and 3.5% NaCl, growth on plates containing 1.5% NaCl, growth in a semisolid minimal medium, anaerobic growth in the presence of 0.1% trimethylamine-N-oxide, hydrogen sulfide production in SIM medium and triple-sugar iron agar, hippurate hydrolysis, nitrite reduction, and growth on plates under an air atmosphere.

scholarly journals Nitrate-Dependent N2O Emission from Intact Soybean Nodules via Denitrification by Bradyrhizobium japonicum Bacteroids

2011 ◽  
Vol 77 (24) ◽  
pp. 8787-8790 ◽  
Author(s):  
Junta Hirayama ◽  
Shima Eda ◽  
Hisayuki Mitsui ◽  
Kiwamu Minamisawa
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Double Mutant ◽  
N2o Emission ◽  
Content Type ◽  
Nitrate N ◽  
Soybean Roots

ABSTRACTIn the presence of nitrate, N2O emission increased markedly from soybean roots inoculated withnosZmutant ofBradyrhizobium japonicum, but not from soybean roots inoculated with anapA nosZdouble mutant, indicating thatB. japonicumbacteroids in soybean nodules are able to convert the exogenously supplied nitrate into N2O via a denitrification pathway.

Genetic diversity in Bradyrhizobium japonicum Jordan 1982 and a proposal for Bradyrhizobium elkanii sp.nov.

1992 ◽  
Vol 38 (6) ◽  
pp. 501-505 ◽  
Author(s):  
L. D. Kuykendall ◽  
B. Saxena ◽  
T. E. Devine ◽  
S. E. Udell
Keyword(s):  
Type Strain ◽  
Homology Group ◽  
Dna Homology ◽  
Homology Groups ◽  
Hybridization Probes ◽  
Bradyrhizobium Elkanii ◽  
Group Ii ◽  
A New Species ◽  
Strain Usda ◽  
Soybean Nodulation

Fourteen randomly selected clones from cosmid libraries of Bradyrhizobium were used as hybridization probes in Southern blot analysis. Seven of the probes used were from strain USDA 83, a group II strain, and the other seven were from strain I-110, a group Ia strain. The 30 strains examined included 9 strains of Rj4-incompatible soybean bradyrhizobia. Considerable polynucleotide sequence dissimilarity between DNA homology groups was evidenced by striking differences in the number of hybridizing bands, except where the probe carried repetitive DNA. Predictable, simple restriction fragment length polymorphism differences were observed only within DNA homology groups. The previous description that 8 of 9 Rj4-incompatible strains belonged to DNA homology group II was verified. The new data, together with many previously documented differences, make it clear that the DNA homology group II organisms should be classified as a new species, for which the name Bradyrhizobium elkanii is proposed, and strain USDA 76 is designated the type strain. The ATCC number for the type strain is 49852. Key words: DNA:DNA hybridization, soybean, nodulation, bacteria, symbiosis, nitrogen fixation, host compatibility.

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