FORMATION OF RIBOMONONUCLEOTIDES FROM PURINE AND PYRIMIDINE BASES AND 5′-PHOSPHORIBOSYLPYROPHOSPHATE BY A SALMON MILT EXTRACT
Keyword(s):
Soluble extracts of immature sockeye salmon milts were prepared by blending them with 0.66 M lithium chloride solution to effect plasmolysis, followed by dilution to 0.10 M concentration with water and removal of the precipitated nucleoprotamine by centrifuging. By means of radioactive tracer technique it was shown that these extracts phosphorylated adenine, guanine, hypoxanthine, thymine, uracil, cytosine, and orotic acid in the presence of α-phosphoribosylpyrophosphate to yield ribomononucleotides.
Keyword(s):
2019 ◽
Vol 92
(8)
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pp. 1113-1121
1965 ◽
Vol 16
(4)
◽
pp. 261-264
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1972 ◽
Vol 36
(1)
◽
pp. 31-40
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