Structural characterization of the capsular polysaccharide and O-chain of the lipopolysaccharide of Actinobacilluspleuropneumoniae serotype 8 (strain 405)

1990 ◽  
Vol 68 (2) ◽  
pp. 329-333 ◽  
Author(s):  
Eleonora Altman ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
Teichoic Acid ◽  
Linear Polymer ◽  
Magnetic Resonance Studies ◽  
Acid Type ◽  
Structure Formula

The capsular polysaccharide of Actinobacilluspleuropneumoniae serotype 8 (strain 405) was found to be a teichoic acid type polysaccharide composed of 2-acetamido-2-deoxy-D-galactose (two parts), glycerol (one part), and phosphate (one part). From hydrolysis, dephosphorylation, methylation, and 1H and 13C nuclear magnetic resonance studies, the polysaccharide was found to be a high molecular weight linear polymer having the structure:[Formula: see text]The lipopolysaccharide O-antigen of A. pleuropneumoniae serotype 8 was shown to be a high molecular weight unbranched linear polymer of a repeating pentasaccharide unit having the structure identical to that produced by A. pleuropneumoniae serotype 3, as follows:[Formula: see text]Keywords: Actinobacilluspleuropneumoniae polysaccharide, lipopolysaccharide, NMR.

The specific capsular polysaccharide of Streptococcus pneumoniae type 15A (American type 30)

1984 ◽  
Vol 62 (2-3) ◽  
pp. 151-161 ◽  
Author(s):  
Martine Caroff ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The specific capsular polysaccharide of Streptococcus pneumoniae type 15A (American type 30) is composed of D-galactose (three parts), D-glucose (one part), 2-acetamido-2-deoxy-D-glucose (one part), phosphate (one part), and glycerol (one part). Hydrolysis, periodate oxidation, methylation, optical rotation, and nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight linear polymer of a pentasaccharide repeating unit having the structure:[Formula: see text]

Structure of the capsular polysaccharide of Haemophilus pleuropneumoniae serotype 3

1987 ◽  
Vol 65 (11) ◽  
pp. 960-967 ◽  
Author(s):  
Eleonora Altman ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer ◽  
Magnetic Resonance Studies ◽  
Structure Formula ◽  
Nuclear Magnetic

The capsular polysaccharide of Haemophilus pleuropneumoniae serotype 3 (ATCC 27090) is composed of D-galactose (one part), 2-acetamido-2-deoxy-D-glucose (one part), glycerol (one part), and phosphate (one part). From hydrolysis, dephosphorylation, methylation, and 1H and 13C nuclear magnetic resonance studies, the polysaccharide was found to be a high molecular weight polymer of a repeating trisaccharide unit, joined through monophosphate diester linkages and having the following structure:[Formula: see text]

Structural studies of the capsular polysaccharide from Actinobacillus (Haemophilus) pleuropneumoniae serotype 4

1988 ◽  
Vol 66 (9) ◽  
pp. 998-1004 ◽  
Author(s):  
Eleonora Altman ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
Structural Studies ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The capsular polysaccharide of Actinobacillus (Haemophilus pleuropneumoniae serotype 4 (ATCC 33378) is composed of D-glucose (one part), 2-acetamido-2-deoxy-D-galactose (one part), and phosphate (one part). From hydrolysis, dephosphorylation, methylation, and 1H and 13C nuclear magnetic resonance studies, the polysaccharide was found to be a high molecular weight polymer of a repeating disaccharide unit, joined through monophosphate diester linkages and having the following structure:[Formula: see text]

Structural studies of the capsular polysaccharide from Haemophilus pleuropneumoniae serotype 2

1987 ◽  
Vol 65 (5) ◽  
pp. 414-422 ◽  
Author(s):  
Eleonora Altman ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
Structural Studies ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer ◽  
Structure Formula ◽  
Nuclear Magnetic

The capsular polysaccharide of Haemophilus pleuropneumoniae serotype 2 (ATCC 27089) is composed of D-glucose (two parts), D-galactose (one part), glycerol (one part), and phosphate (one part). Hydrolysis, dephosphorylation, methylation, enzymic studies, and 1H and 13C nuclear magnetic resonance experiments showed that the polysaccharide is a high molecular weight polymer of a tetrasaccharide repeating units, linked by monophosphate diester and having the following structure:[Formula: see text]

The specific capsular polysaccharide of Streptococcus pneumoniae type 33F

1984 ◽  
Vol 62 (8) ◽  
pp. 666-677 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry ◽  
Peter J. Kniskern
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The specific capsular polysaccharide of Streptococcus pneumoniae type 33F (American type 70) is composed of D-galactose (5 parts), D-glucose (1 part), and O-acetyl (ca. 0.4 parts). Periodate oxidation, partial hydrolysis, and 1H and 13C nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight polymer of a repeating hexasaccharide unit having the structure:[Formula: see text]

The structures of the two lipopolysaccharide O-chains produced by Salmonella boecker

1988 ◽  
Vol 66 (10) ◽  
pp. 1066-1077 ◽  
Author(s):  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Nitrous Acid ◽  
Optical Rotation ◽  
Partial Hydrolysis ◽  
Magnetic Resonance Studies ◽  
High Molecular Weight Polymers ◽  
Nuclear Magnetic

Salmonella boecker, which belongs to group 0:6, 14(H) and shows the antigenic factors 6, 14, [1], and [25], defined by the Kauffmann–White system, produces two lipopolysaccharides differing from each other in the structures of their 0-polysaccharide moieties. By glycose composition, partial hydrolysis, nitrous acid deamination, methylation, optical rotation, and 1H and 13C nuclear magnetic resonance studies, the O-polysaccharides were demonstrated to be high-molecular-weight polymers (I and II) composed of either structurally related repeating tetrasaccharide or repeating pentasaccharide units having the structuresand[Formula: see text][Formula: see text]

Structure of the acidic capsular polysaccharide of Cryptococcus laurentii (NRRL Y-1401)

1982 ◽  
Vol 60 (2) ◽  
pp. 124-130 ◽  
Author(s):  
Malcolm B. Perry ◽  
Ann C. Webb
Keyword(s):  
Molecular Weight ◽  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Regular Structure ◽  
Cryptococcus Laurentii ◽  
Partial Acid Hydrolysis ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The capsular polysaccharide produced by Cryptococcus laurentii (NRRL Y-1401) is composed of D-mannose (3 mol), D-glucuronic acid (1 mol), D-xylose (1 mol), and O-acetyl (~1 mol). Methylation, periodate oxidation, partial acid hydrolysis, optical rotation, and nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight branched polymer of regular structure having a repeating pentasaccharide unit with the structure:[Formula: see text]

Structure of the lipopolysaccharide antigenic O-chain produced by Salmonella livingstone (O:6,7)

1989 ◽  
Vol 67 (6) ◽  
pp. 278-280 ◽  
Author(s):  
Jose Luis Di Fabio ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Key Words ◽  
Methylation Analysis ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The lipopolysaccharide produced by Salmonella livingstone (O:6,7) was composed of an antigenic O-polysaccharide which was shown by composition, methylation analysis and high resolution nuclear magnetic resonance studies to be a high molecular weight polymer containing D-glucose, 2-acetamido-2-deoxy-D-glucose and D-mannose residues (1:1:4) composed in a repeating hexasaccharide unit having the structure:[Formula: see text]Key words: Salmonella livingstone, lipopolysaccharide, O-polysaccharide.

The specific capsular polysaccharide of Streptococcus pneumoniae type 20

1983 ◽  
Vol 61 (4) ◽  
pp. 178-190 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry ◽  
Dennis J. Carlo
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer

The specific capsular polysaccharide produced by Streptococcus pneumoniae type 20 is composed of D-glucose (three parts), D-galactose (two parts), 2-acetarnido-2-deoxy-D-glucose (one part), phosphate (one part), and O-acetyl (approximately two parts). Methylation, periodate oxidation, optical rotation, nuclear magnetic resonance, partial and enzymic hydrolyses, and chromatographic studies showed that the polysaccharide is a high molecular weight polymer of partially O-acetylated hexasaccharide repeating units linked by monophosphate, having the structure indicated below:[Formula: see text]

Structural studies of the capsular polysaccharide from Haemophilus pleuropneumoniae serotype l

1986 ◽  
Vol 64 (8) ◽  
pp. 707-716 ◽  
Author(s):  
Eleonora Altman ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Capsular Polysaccharide ◽  
Teichoic Acid ◽  
Proton Nuclear Magnetic Resonance ◽  
Partial Hydrolysis ◽  
Composition Analysis ◽  
Acid Type ◽  
Serotype 1 ◽  
Nuclear Magnetic

The capsular polysaccharide of Haemophilus pleuropneumoniae serotype 1 (ATCC 27088) was found to be a teichoic acid type polysaccharide of a repeating disaccharide unit composed of 2-acetamido-2-deoxy-D-glucose and D-galactose units. By composition analysis, methylation, partial hydrolysis, dephosphorylation, and one- and two-dimensional 500-MHz proton nuclear magnetic resonance experiments, together with 13C nuclear magnetic resonance studies, it was concluded that the capsular polysaccharide is a high molecular weight linear polymer having the structure:[Formula: see text]

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