The effect of reinnervation on the distribution of muscle fibre types in the tibialis anterior muscle of the mouse

The distribution of fibre types in the tibialis anterior (TA) muscle of adult mice was examined by means of an immuno-histochemical approach, using monoclonal antibodies that recognize different myosin heavy chain isoforms. As has been reported previously, the superficial portion of TA contains almost exclusively type IIB fibres and is almost entirely glycolytic in nature. Following section of the lateral popliteal nerve and rotation of the proximal stomp to prevent rematching, it was found that the original pattern was virtually restored within 2 months. One possible explanation for this observation is that the activity pattern of peripheral and deep muscle fibres differs and that this aids in specification of muscle fibre type. Alternatively, the muscle fibres of the superficial portion of TA may be inherently resistant to an alteration of their phenotype with regard to expression of myosin heavy chain.Key words: myosin heavy chain, immunohistochemistry, plasticity, self-reinnervation, fibre types.

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Four novel myosin heavy chain transcripts define a molecular basis for muscle fibre types inRanapipiens

The Journal of Physiology ◽

10.1111/j.1469-7793.1998.667bp.x ◽

1998 ◽

Vol 508 (3) ◽

pp. 667-680 ◽

Cited By ~ 32

Author(s):

Gordon J. Lutz ◽

Denise B. Cuizon ◽

Allen F. Ryan ◽

Richard L. Lieber

Keyword(s):

Myosin Heavy Chain ◽

Muscle Fibre ◽

Heavy Chain ◽

Molecular Basis ◽

Fibre Types ◽

Muscle Fibre Types

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Three myosin heavy chain isoforms in type 2 skeletal muscle fibres

Journal of Muscle Research and Cell Motility ◽

10.1007/bf01739810 ◽

1989 ◽

Vol 10 (3) ◽

pp. 197-205 ◽

Cited By ~ 612

Author(s):

Stefano Schiaffino ◽

Luisa Gorza ◽

Saverio Sartore ◽

Leopoldo Saggin ◽

Simonetta Ausoni ◽

...

Keyword(s):

Skeletal Muscle ◽

Myosin Heavy Chain ◽

Heavy Chain ◽

Myosin Heavy Chain Isoforms ◽

Muscle Fibres ◽

Skeletal Muscle Fibres

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Pattern of Myosin Heavy Chain Isoforms in Different Fibre Types of Canine Trunk and Limb Skeletal Muscles

Cells Tissues Organs ◽

10.1159/000077034 ◽

2004 ◽

Vol 176 (4) ◽

pp. 178-186 ◽

Cited By ~ 13

Author(s):

M. Štrbenc ◽

V. Smerdu ◽

M. Županc ◽

N. Tozon ◽

G. Fazarinc

Keyword(s):

Myosin Heavy Chain ◽

Heavy Chain ◽

Skeletal Muscles ◽

Myosin Heavy Chain Isoforms ◽

Fibre Types

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Two functionally distinct myosin heavy chain isoforms in slow skeletal muscle fibres

FEBS Letters ◽

10.1016/s0014-5793(97)00556-5 ◽

1997 ◽

Vol 410 (2-3) ◽

pp. 150-152 ◽

Cited By ~ 37

Author(s):

Stefan Galler ◽

Karlheinz Hilber ◽

Bärbel Gohlsch ◽

Dirk Pette

Keyword(s):

Skeletal Muscle ◽

Myosin Heavy Chain ◽

Heavy Chain ◽

Myosin Heavy Chain Isoforms ◽

Muscle Fibres ◽

Slow Skeletal Muscle ◽

Skeletal Muscle Fibres

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Fibre types in the skeletal muscle of blackspot seabream (Pagellus bagaraveo, Brunnich) juveniles: a histochemical, immunohistochemical and ultrastructural study

Microscopy and Microanalysis ◽

10.1017/s1431927609990687 ◽

2009 ◽

Vol 15 (S3) ◽

pp. 43-44

Author(s):

P. Silva ◽

L. M. P. Valente ◽

F. Malhão ◽

M. H. Galante ◽

R. A. F. Monteiro ◽

...

Keyword(s):

Muscle Fibre ◽

Main Part ◽

Histochemical Staining ◽

Fibre Types ◽

Muscle Fibre Types ◽

Muscle Fibres ◽

Potential Candidate ◽

Mitochondrial Content ◽

Narrow Strip ◽

Specific Antibodies

AbstractThe aim of this work was to gain insights into the muscle fibre phenotype and ultrastructure in blackspot seabream juveniles, a potential candidate for Portuguese aquaculture. Firstly, we used a panel of isoform-specific antibodies as well as histochemical staining for myosin ATPase and for SDH enzyme activity (a marker for mitochondrial content and oxidative metabolism) to identify myosin expression. Secondly, muscle fibre types were also identified by their fine structure where powerful approaches contributed to understand the muscle fibre phenotype of blackspot seabream juveniles. We observed that the main part of the musculature consisted of fast-white fibres, with a narrow strip of slow-red fibres situated superficially adjacent to the lateral line. A zone of intermediate-pink muscle fibres was inserted between those muscle domains (Fig.1).

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Organization of skeletal muscle in the urodele Triturus cristatus : muscle fibre types and motor units

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1985.0015 ◽

1985 ◽

Vol 223 (1233) ◽

pp. 495-510 ◽

Cited By ~ 4

Keyword(s):

Succinate Dehydrogenase ◽

Motor Unit ◽

Muscle Fibre ◽

Lucifer Yellow ◽

Motor Units ◽

Fibre Types ◽

Differential Staining ◽

Muscle Fibre Types ◽

Muscle Fibres ◽

Dehydrogenase Reaction

Four muscle fibre types are described in the biceps and extensor digitorum communis muscles of the newt forelimb. The histological criteria forming the basis for the distinctions include differential staining with p -phenylenediamine and succinate dehydrogenase histochemistry and electron microscopy. In addition, three distinctive motor unit types are described for the biceps muscle. These are fast units, slow units and intermediate units. The structure of muscle fibre and the physiological characteristics of muscle fibres belonging to each motor unit, have been correlated by using iontophoretic passage of Lucifer yellow into muscle fibres belonging to physiologically characterized motor units and their subsequent histological identification by the succinate dehydrogenase reaction. The three motor unit types correspond to slow muscle fibres, intermediate muscle fibres and two classes of fast muscle fibres.

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The turnover of cytochrome c in different skeletal-muscle fibre types of the rat

Biochemical Journal ◽

10.1042/bj1780569 ◽

1979 ◽

Vol 178 (3) ◽

pp. 569-574 ◽

Cited By ~ 31

Author(s):

R L Terjung

Keyword(s):

Skeletal Muscle ◽

Cytochrome C ◽

Muscle Fibre ◽

Skeletal Muscle Fibre ◽

Fibre Types ◽

Male Rats ◽

Muscle Fibre Types ◽

Muscle Fibres ◽

Double Labelling ◽

Fast Twitch

The turnover of cytochrome c was determined in the three skeletal-muscle fibre types of adult male rats by a kinetic analysis that followed the time course of cytochrome c content change. Confirming evidence was obtained with double-labelling studies using delta-aminolaevulinate. Cytochrome c turnover was most rapid in the low-oxidative fast-twitch white fibre [t1/2 (half-life) about 4 days], slowest in the high-oxidative fast-twitch red fibre (t1/2 9-10 days) and relatively rapid in the high-oxidative slow-twitch red fibre (t1/2 5-6 days). Thus cytochrome c turnover does not strictly conform to either the appearance (i.e. red or white) or the contractile characteristics (i.e. fast or slow) of the muscle fibres. The synthesis rates needed to maintain the corresponding cytochrome c concentrations, however, were similarly high in the two mitochondria-rich red fibre types. These data illustrate that both the synthesis and degradation processes are important in establishing the cytochrome c concentrations that distinguish the different skeletal-muscle fibre types.

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Preferred metabolic pathway of bovine muscle fibre revealed by synchrotron–deep ultraviolet fluorescence imaging

Journal of Spectral Imaging ◽

10.1255/jsi.2019.a14 ◽

2019 ◽

Author(s):

Thierry Astruc ◽

Olivier Loison ◽

Frédéric Jamme ◽

Matthieu Réfrégiers ◽

Annie Vénien

Keyword(s):

Fluorescence Imaging ◽

Muscle Fibre ◽

Cross Sections ◽

Metabolic Pathway ◽

Fibre Types ◽

Muscle Fibre Types ◽

Muscle Fibres ◽

Label Free ◽

Bovine Muscle ◽

Deep Ultraviolet

The different bovine muscle fibre types I, IIA and IIX are characterised by their preferred metabolic pathway, either oxidative (I, IIA) or glycolytic (IIX), and their contraction speed, either slow-twitch (I) or fast-twitch (IIA, IIX). These physiological specificities are associated with variations in intracellular composition and their fluorescence spectra signatures. We hypothesised that these slight differences in autofluorescence responses could be used to discriminate the muscle fibre types by fluorescence imaging. Serial histological cross-sections of beef longissimus dorsi were performed: the start set was used to identify the metabolic and contractile type of muscle fibres by both immunohistoenzymology and immunohistofluorescence, and the following set was used to acquire synchrotron–deep ultraviolet (UV) autofluorescence images after excitation in the UV range (275 nm and 315 nm). This strategy made it possible to explore the label-free autofluorescence of muscle cells previously subtyped by histochemistry. Glycolytic cells (IIX) showed more intense fluorescence than oxidative cells (I and IIA) with near-90 % accuracy. This discrimination is more specifically assigned to the fluorescence of nicotinamide adenine dinucleotide. UV autofluorescence was unable to discriminate contractile type.

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