The rat parietal cell: canaliculi and tubulovesicles

1974 ◽  
Vol 52 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Thomas S. Leeson
Keyword(s):  
Parietal Cell ◽  
Light And Electron Microscopy ◽  
Rapid Freezing ◽  
Chemical Fixation ◽  
Membrane Systems ◽  
Membrane Flow ◽  
Secretory Cycle ◽  
Gastric Parietal Cell ◽  
Membrane Breakdown ◽  
Cytological Features

The cytological features of the gastric parietal cell of the rat have been described using light and electron microscopy of material fixed either directly or after previous rapid freezing. Appearances have been correlated with the secretory cycle and particular emphasis placed on the conformation and interrelation of cytoplasmic tubulovesicles and intracellular canaliculi. The possible continuity of these membrane systems and their importance in acid secretion have been discussed in relation to the effectiveness of rapid freezing in preservation of vital relations. It is concluded that rapid freezing before chemical fixation does produce material which more accurately reflects vital appearances than does routine chemical fixation; and further evidence is provided for the theory of membrane flow during the secretory cycle of the parietal cell. Further, the possibility of membrane breakdown and reformation during the cycle is discussed.

scholarly journals Cytochemical properties of mitochondria in the gastric parietal cell.

1979 ◽  
Vol 27 (4) ◽  
pp. 873-877 ◽  
Author(s):  
P L Sannes ◽  
T Katsuyama ◽  
S S Spicer
Keyword(s):  
Cytochrome Oxidase ◽  
Parietal Cell ◽  
Light And Electron Microscopy ◽  
Intermembrane Space ◽  
Gastric Parietal Cell ◽  
High Iron ◽  
Acidic Complex ◽  
The Matrix ◽  
Complex Carbohydrates ◽  
Gastric Parietal Cells

The matrix of some mitochondria in gastric parietal cells of rat and guinea pig evidenced affinity for the high iron diamine method which localizes sulfated complex carbohydrates selectively by light and electron microscopy. Such staining has not been observed elsewhere in the stomach. The high iron diamine reactive mitochondria about equaled in number those which were unreactive, and the two groups were indistinguishable morphologically. The distinction was not apparent either when mitochondria were stained by other cytochemical procedures including dialyzed iron for acidic complex carbohydrates, 3-3' diaminobenzidine-H2O2 at pH 6.0 for cytochrome oxidase, and Kominick's pyroantimonate osmium tetroxide for antimonate precipitable cations. The dialyzed iron method stained acid glycoconjugates in the outer intermembrane space in parietal cell mitochondria. These mitochondria stained more strongly with dialyzed iron than have any others examined heretofore with this method and comprised the only reactive mitochondria in the stomach. Parietal cell mitochondria also stained strongly for cytochrome oxidase but those of other gastric cells failed to evidence this reactivity.

Fatty Acids in a High-Fat Diet Potentially Induce Gastric Parietal-Cell Damage and Metaplasia in Mice

2017 ◽  
Vol 152 (5) ◽  
pp. S418
Author(s):  
Yuki Hirata ◽  
Shinji Fukuda ◽  
Kazuhiko Yamada ◽  
Kazuhide Higuchi ◽  
Yuki I. Kawamura ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Parietal Cell ◽  
High Fat Diet ◽  
Cell Damage ◽  
High Fat ◽  
Gastric Parietal Cell

scholarly journals The Lamellar Systems of Cytoplasmic Membranes in Dividing Spermatogenic Cells of Drosophila virilis

1960 ◽  
Vol 7 (3) ◽  
pp. 433-441 ◽  
Author(s):  
Susumu Ito
Keyword(s):  
Endoplasmic Reticulum ◽  
Golgi Complex ◽  
Light And Electron Microscopy ◽  
Primary Spermatocyte ◽  
Drosophila Virilis ◽  
Spermatogenic Cells ◽  
Membrane Systems ◽  
Cytoplasmic Membranes ◽  
Pas Reaction ◽  
Staining Methods

Spermatogenic cells of Drosophila virilis were studied by light and electron microscopy. The persistence of a "nuclear wall" during the meiotic divisions has been reported by a number of early cytologists, but this interpretation has been a subject of debate. Electron micrographs of dividing spermatocytes reveal the presence of multiple layers of paired membranes surrounding the nuclear region. These lamellar membrane systems are not typical of the nuclear envelope, but were interpreted as such by light microscopists. The membranes constituting a pair are separated by an interspace of ∼ 100 A and successive pairs are 200 to 400 A apart. These spacings are similar but not identical to those found in the lamellar systems of the Golgi complex. The cisternae of the endoplasmic reticulum in this material are devoid of attached ribonucleoprotein particles, are more precisely ordered than in vertebrate cells, and show a uniform, narrow intracisternal space of ∼ 100 A. The conspicuous asters appear to be made up of similar paired membranes radiating from the centriolar region. The primary spermatocyte has numerous dictyosomes and a well developed endoplasmic reticulum in cisternal form, but no typical Golgi complex or endoplasmic reticulum is found during the meiotic division stages of metaphase to telophase. Evidence is presented that these cytoplasmic organelles contribute to the formation of the extensive lamellar systems that appear during meiosis. The results of the Golgi silver staining methods and staining tests for phospholipids, basophilia, and the PAS reaction, indicate that the lamellar arrays of membranes present during meiosis are indistinguishable from the Golgi complex in their tinctorial properties.

scholarly journals Anemia and hematinic deficiencies in gastric parietal cell antibody-positive and -negative oral mucosal disease patients with microcytosis

2017 ◽  
Vol 116 (8) ◽  
pp. 613-619 ◽  
Author(s):  
Hung-Pin Lin ◽  
Yu-Hsueh Wu ◽  
Yi-Ping Wang ◽  
Yang-Che Wu ◽  
Julia Yu-Fong Chang ◽  
...  
Keyword(s):  
Parietal Cell ◽  
Gastric Parietal Cell ◽  
Mucosal Disease ◽  
Cell Antibody ◽  
Parietal Cell Antibody

3-Dimensional Modeling of the Gastric Parietal Cell

2002 ◽  
pp. 295-304
Author(s):  
John G. Forte ◽  
Joseph G. Duman ◽  
Nimesh J. Pathak ◽  
Kent L. Mcdonald
Keyword(s):  
Parietal Cell ◽  
Gastric Parietal Cell ◽  
3 Dimensional ◽  
Dimensional Modeling

Gastric parietal cell development: Expression of the H+/K+ATPase subunits coincides with the biogenesis of the secretory membranes

1993 ◽  
Vol 71 (3) ◽  
pp. 191-200 ◽  
Author(s):  
JOHN M. PETTITT ◽  
BAN-HOCK TOH ◽  
JUDY M. CALLAGHAN ◽  
PAUL A. GLEESON ◽  
IAN R. VAN DRIEL
Keyword(s):  
Parietal Cell ◽  
Cell Development ◽  
Gastric Parietal Cell ◽  
Atpase Subunits

Self-pressurised rapid freezing, a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes

Nematology ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 103-110
Author(s):  
Myriam Claeys ◽  
Nurul Dwi Handayani ◽  
Vladimir V. Yushin ◽  
Prabowo Lestari ◽  
Antarjo Dikin ◽  
...  
Keyword(s):  
High Pressure ◽  
Fast Rate ◽  
Low Cost ◽  
Heterodera Schachtii ◽  
Fixation Method ◽  
Rapid Freezing ◽  
Chemical Fixation ◽  
High Pressure Freezing ◽  
Cyst Nematodes ◽  
Second Stage

Summary Ultrastructural analysis of the early development of nematodes is hampered by the impermeability of the eggshell to most commonly used fixatives. High-pressure freezing (HPF), a physical cryo-fixation method, facilitates a fast rate of fixation, and by using this method the issue of the uneven delivery of fixative is circumvented. Although HPF results in a superior preservation of the fine structure, the equipment costs impede a wider application of this method. Self-pressurised rapid freezing (SPRF) is an alternative low-cost cryo-fixation method, and its usefulness was evaluated in an ultrastructural study of the eggshell and the cuticle of unhatched second-stage juveniles (J2) of Globodera rostochiensis and Heterodera schachtii. A comparison with conventional (chemical) fixation demonstrates that SPRF fixation results in a remarkably well-preserved ultrastructure of the entire egg including both the eggshell and the cellular details of the unhatched J2. Therefore, SPRF fixation is proposed as an affordable, relatively easy-to-use and time-efficient technique to study the ultrastructure of unhatched J2 and eggs of nematodes.

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