Indirect calorimetry measurements of behavioral thermoregulation in a semiaquatic social rodent, Myocastor coypus

1992 ◽  
Vol 70 (5) ◽  
pp. 907-911 ◽  
Author(s):  
Christine Moinard ◽  
C. Patrick Doncaster ◽  
Hervé Barré
Keyword(s):  
Metabolic Rate ◽  
Indirect Calorimetry ◽  
Cold Water ◽  
Resting Metabolic Rate ◽  
Behavioral Thermoregulation ◽  
Myocastor Coypus ◽  
Thermoneutral Zone ◽  
Different Temperatures ◽  
Males And Females

The role of behavior is quantified in the maintenance of thermic homeostasis by a semiaquatic rodent, the coypu (Myocastor coypus). Indirect calorimetry measurements showed that huddling behavior is responsible for a 20% drop in resting metabolic rate at temperatures below the thermoneutral zone. Different associations of males and females in groups of two and three resulted in significant differences in decreases of resting metabolic rate. In a cold environment (Ta = −5 °C), males used a ball-like posture significantly more often than females. When presented with a choice of substrates (air versus water) at different temperatures, females tended to reduce their time spent in cold water but males did not, despite a greater loss of energy in water compared with air. These results appear to express a conflict between social and thermoregulatory behaviors, contributing to male-biased mortality in winter.

Percent relative cumulative frequency analysis in indirect calorimetry: application to studies of transgenic mice

2004 ◽  
Vol 82 (12) ◽  
pp. 1075-1083 ◽  
Author(s):  
Marc Riachi ◽  
Jean Himms-Hagen ◽  
Mary-Ellen Harper
Keyword(s):  
Oxygen Consumption ◽  
Energy Expenditure ◽  
Metabolic Rate ◽  
Indirect Calorimetry ◽  
Uncoupling Protein ◽  
Resting Metabolic Rate ◽  
Large Data ◽  
Cumulative Frequency ◽  
And Control ◽  
Deficient Mice

Indirect calorimetry is commonly used in research and clinical settings to assess characteristics of energy expenditure. Respiration chambers in indirect calorimetry allow measurements over long periods of time (e.g., hours to days) and thus the collection of large sets of data. Current methods of data analysis usually involve the extraction of only a selected small proportion of data, most commonly the data that reflects resting metabolic rate. Here, we describe a simple quantitative approach for the analysis of large data sets that is capable of detecting small differences in energy metabolism. We refer to it as the percent relative cumulative frequency (PRCF) approach and have applied it to the study of uncoupling protein-1 (UCP1) deficient and control mice. The approach involves sorting data in ascending order, calculating their cumulative frequency, and expressing the frequencies in the form of percentile curves. Results demonstrate the sensitivity of the PRCF approach for analyses of oxygen consumption ([Formula: see text]02) as well as respiratory exchange ratio data. Statistical comparisons of PRCF curves are based on the 50th percentile values and curve slopes (H values). The application of the PRCF approach revealed that energy expenditure in UCP1-deficient mice housed and studied at room temperature (24 °C) is on average 10% lower (p < 0.0001) than in littermate controls. The gradual acclimation of mice to 12 °C caused a near-doubling of [Formula: see text] in both UCP1-deficient and control mice. At this lower environmental temperature, there were no differences in [Formula: see text] between groups. The latter is likely due to augmented shivering thermogenesis in UCP1-deficient mice compared with controls. With the increased availability of murine models of metabolic disease, indirect calorimetry is increasingly used, and the PRCF approach provides a novel and powerful means for data analysis.Key words: thermogenesis, oxygen consumption, metabolic rate, uncoupling protein, UCP.

scholarly journals Indirect Calorimetry Protocol Development for Measuring Resting Metabolic Rate as a Component of Total Energy Expenditure in Free-Living Postmenopausal Women

2001 ◽  
Vol 131 (8) ◽  
pp. 2215-2218 ◽  
Author(s):  
Neilann K. Horner ◽  
Johanna W. Lampe ◽  
Ruth E. Patterson ◽  
Marian L. Neuhouser ◽  
Shirley A. Beresford ◽  
...  
Keyword(s):  
Energy Expenditure ◽  
Metabolic Rate ◽  
Total Energy ◽  
Postmenopausal Women ◽  
Indirect Calorimetry ◽  
Resting Metabolic Rate ◽  
Total Energy Expenditure ◽  
Free Living ◽  
Protocol Development

scholarly journals Temperature Is Sufficient to Condition a Flavor Preference for a Cold-Paired Solution in Rats

2020 ◽  
Vol 45 (7) ◽  
pp. 563-572
Author(s):  
Kristen E Kay ◽  
Laura E Martin ◽  
Kimberly F James ◽  
Sashel M Haygood ◽  
Ann-Marie Torregrossa
Keyword(s):  
Home Cage ◽  
Cold Water ◽  
Cold Temperature ◽  
Warm Water ◽  
Flavor Preference ◽  
Different Temperatures ◽  
Stimulus Temperature ◽  
Preference Tests

Abstract Increasing evidence suggests that stimulus temperature modifies taste signaling. However, understanding how temperature modifies taste-driven behavior is difficult to separate as we must first understand how temperature alone modifies behavior. Previous work has suggested that cold water is more rewarding and “satiating” than warm water, and water above orolingual temperature is avoided in brief-access testing. We explored the strength of cold water preference and warm water avoidance by asking: (1) if cold temperature alone was sufficient to condition a flavor preference and (2) if avoidance of warm stimuli is driven by novelty. We addressed these questions using custom-designed equipment that allows us to monitor and maintain solution temperatures. We conducted two-bottle preference tests, after pairing Kool-Aid flavors with 10 or 40 °C. Rats preferred the flavor paired with cold temperature, both while it was cold and for 1 day while solutions were presented at 22 °C. We then examined the role of novelty in avoidance of 40 °C. Rats were maintained on 10, 22, or 40 °C water in their home cage to increase familiarity with the temperatures. Rats were then subject to a series of brief-access taste tests to water or sucrose at 10 to 40 °C. Rats that had 40 °C experience licked more to 40 °C water, but not sucrose, during brief-access testing. In a series of two-bottle preference tests, rats maintained on 40 °C water had a decreased preference for 10 °C water when paired opposite 40 °C water. Together, these data contribute to our understanding of orosensory-driven behavior with water at different temperatures.

scholarly journals Resting Metabolic Rate in Elite Rowers and Canoeists: Difference between Indirect Calorimetry and Prediction

2011 ◽  
Vol 58 (3) ◽  
pp. 239-244 ◽  
Author(s):  
Anja Carlsohn ◽  
Friederike Scharhag-Rosenberger ◽  
Michael Cassel ◽  
Frank Mayer
Keyword(s):  
Metabolic Rate ◽  
Indirect Calorimetry ◽  
Resting Metabolic Rate ◽  
Elite Rowers

scholarly journals The Energy Expenditure of Free-Living Physical Activities in Primary Schoolchildren

2016 ◽  
Vol 13 (s1) ◽  
pp. S57-S61 ◽  
Author(s):  
Alison L. Innerd ◽  
Liane B. Azevedo
Keyword(s):  
Physical Activity ◽  
Energy Expenditure ◽  
Metabolic Rate ◽  
Indirect Calorimetry ◽  
Resting Metabolic Rate ◽  
Physical Activities ◽  
Free Living ◽  
Metabolic Equivalent ◽  
Primary Schoolchildren ◽  
Vigorous Intensity

Background:The aim of this study is to establish the energy expenditure (EE) of a range of child-relevant activities and to compare different methods of estimating activity MET.Methods:27 children (17 boys) aged 9 to 11 years participated. Participants were randomly assigned to 1 of 2 routines of 6 activities ranging from sedentary to vigorous intensity. Indirect calorimetry was used to estimate resting and physical activity EE. Activity metabolic equivalent (MET) was determined using individual resting metabolic rate (RMR), the Harrell-MET and the Schofield equation.Results:Activity EE ranges from 123.7± 35.7 J/min/Kg (playing cards) to 823.1 ± 177.8 J/min/kg (basketball). Individual RMR, the Harrell-MET and the Schofield equation MET prediction were relatively similar at light and moderate but not at vigorous intensity. Schofield equation provided a better comparison with the Compendium of Energy Expenditure for Youth.Conclusion:This information might be advantageous to support the development of a new Compendium of Energy Expenditure for Youth.

scholarly journals Melanin-concentrating hormone in peripheral circulation in the human

2017 ◽  
Vol 232 (3) ◽  
pp. 513-523 ◽  
Author(s):  
J Naufahu ◽  
F Alzaid ◽  
M Fiuza Brito ◽  
B Doslikova ◽  
T Valencia ◽  
...  
Keyword(s):  
Energy Homeostasis ◽  
Reference Range ◽  
Resting Metabolic Rate ◽  
Peripheral Circulation ◽  
Reference Ranges ◽  
Older Individuals ◽  
Rp Hplc ◽  
Melanin Concentrating Hormone ◽  
Males And Females

Melanin-concentrating hormone (MCH) is a hypothalamic neuropeptide with a well-characterised role in energy homeostasis and emergent roles in diverse physiologic functions such as arousal, mood and reproduction. Work to date has predominantly focused on its hypothalamic functions using animal models; however, little attention has been paid to its role in circulation in humans. The aims of this study were to (a) develop a radioimmunoassay for the detection of MCH in human plasma; (b) establish reference ranges for circulating MCH and (c) characterise the pattern of expression of circulating MCH in humans. A sensitive and specific RIA was developed and cross-validated by RP-HPLC and MS. The effective range was 19.5–1248 pg MCH/mL. Blood samples from 231 subjects were taken to establish a reference range of 19.5–55.4 pg/mL for fasting MCH concentrations. There were no significant differences between male and female fasting MCH concentrations; however, there were correlations between MCH concentrations and BMI in males and females with excess fat (P < 0.001 and P = 0.020) and between MCH concentrations and fat mass in females with excess fat (P = 0.038). Plasma MCH concentrations rose significantly after feeding in a group of older individuals (n = 50, males P = 0.006, females P = 0.023). There were no robust significant correlations between fasting or post-prandial MCH and resting metabolic rate, plasma glucose, insulin or leptin concentrations although there were correlations between circulating MCH and leptin concentrations in older individuals (P = 0.029). These results indicate that the role of circulating MCH may not be reflective of its regulatory hypothalamic role.

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